Pectinolytic enzymes that catalyze the breakdown of substrates containing pectin are widespread. Pectinases have potential applications in various industries, including food, animal feed, textile, paper, and fuel. In this study, one hundred bacterial isolates were collected from Marand city farmlands (Azarbaijan-E-Sharqi, Iran) and screened by MP medium on the base of pectinase activity considering the significance of pectinases. The results depicted that three isolates showed the most pectinase activity (more massive halo). The biochemical and molecular test results showed that the three screened bacteria were Enterobacter and named Enterobacter sp. MF41, Enterobacter sp. MF84, and Enterobacter sp. MF90. Enterobacter sp. MF84 had the largest halo, so this strain was selected for the study of its produced pectinase. The results exhibited that the produced enzyme has optimum temperature and pH for activity at 30 °C and in 9, respectively. Finally, the enzyme production by Enterobacter sp. MF84 is optimized using response surface methodology (RSM) considering four factors (NH4Cl, K2HPO4, pectin, and incubation time) as variables. The results showed that the optimization procedure increased the enzyme production up to 12 times (from 1.16 to 14.16 U/mg). The Pareto analysis revealed that ammonium chloride has a significant role in decreasing the enzyme production, probably by inducing the nitrification pathway enzymes in the presence of organic nitrogen in Enterobacter sp. MF84.
- MeSH
- Bacteria klasifikace genetika izolace a purifikace metabolismus MeSH
- bakteriální proteiny metabolismus MeSH
- Enterobacter klasifikace genetika izolace a purifikace metabolismus MeSH
- farmy MeSH
- fermentace MeSH
- fylogeneze MeSH
- koncentrace vodíkových iontů MeSH
- kultivační média chemie MeSH
- pektiny analýza metabolismus MeSH
- polygalakturonasa metabolismus MeSH
- půdní mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- statistické modely MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Írán MeSH
Histochemical methods allow for identification and localization of various components within the tissue. Such information on the spatial heterogeneity is not available with biochemical methods. However, there is limitation of the specificity of such detection in context of complex tissue, which is important to consider, and interpretations of the results should regard suitable control treatments if possible. Such methods are valuable extension to specific optical and spectroscopic analytical methods. Here we present a set of selected simple methods of staining and histochemical tests with comments based on our laboratory experience.
- MeSH
- barvení a značení metody MeSH
- barvicí látky analýza MeSH
- buněčná stěna chemie ultrastruktura MeSH
- celulosa analýza MeSH
- histocytochemie metody MeSH
- lignin analýza MeSH
- lipidy analýza MeSH
- mikroskopie metody MeSH
- pektiny analýza MeSH
- rostliny chemie ultrastruktura MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- cholesterol krev MeSH
- hypolipidemika MeSH
- morčata MeSH
- pektiny analýza MeSH
- triglyceridy krev MeSH
- Check Tag
- morčata MeSH
