Pectinolytic enzymes that catalyze the breakdown of substrates containing pectin are widespread. Pectinases have potential applications in various industries, including food, animal feed, textile, paper, and fuel. In this study, one hundred bacterial isolates were collected from Marand city farmlands (Azarbaijan-E-Sharqi, Iran) and screened by MP medium on the base of pectinase activity considering the significance of pectinases. The results depicted that three isolates showed the most pectinase activity (more massive halo). The biochemical and molecular test results showed that the three screened bacteria were Enterobacter and named Enterobacter sp. MF41, Enterobacter sp. MF84, and Enterobacter sp. MF90. Enterobacter sp. MF84 had the largest halo, so this strain was selected for the study of its produced pectinase. The results exhibited that the produced enzyme has optimum temperature and pH for activity at 30 °C and in 9, respectively. Finally, the enzyme production by Enterobacter sp. MF84 is optimized using response surface methodology (RSM) considering four factors (NH4Cl, K2HPO4, pectin, and incubation time) as variables. The results showed that the optimization procedure increased the enzyme production up to 12 times (from 1.16 to 14.16 U/mg). The Pareto analysis revealed that ammonium chloride has a significant role in decreasing the enzyme production, probably by inducing the nitrification pathway enzymes in the presence of organic nitrogen in Enterobacter sp. MF84.

• MeSH
• Bacteria klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• bakteriální proteiny metabolismus   MeSH
• Enterobacter klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• farmy MeSH
• fermentace MeSH
• fylogeneze MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média chemie   MeSH
• pektiny analýza   metabolismus   MeSH
• polygalakturonasa metabolismus   MeSH
• půdní mikrobiologie MeSH
• RNA ribozomální 16S genetika   MeSH
• statistické modely MeSH
• teplota MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Írán MeSH