Assessment of the effective impact of bisphenols on mitochondrial activity and steroidogenesis in a dose-dependency in mice TM3 Leydig cells
Language English Country Czech Republic Media print-electronic
Document type Journal Article
PubMed
31342755
DOI
10.33549/physiolres.934200
PII: 934200
Knihovny.cz E-resources
- MeSH
- Benzhydryl Compounds administration & dosage toxicity MeSH
- Cell Line MeSH
- Endocrine Disruptors administration & dosage toxicity MeSH
- Estrogens, Non-Steroidal administration & dosage toxicity MeSH
- Phenols administration & dosage toxicity MeSH
- Leydig Cells drug effects metabolism MeSH
- Mitochondria drug effects metabolism MeSH
- Mice MeSH
- Testosterone antagonists & inhibitors metabolism MeSH
- Cell Survival drug effects physiology MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Benzhydryl Compounds MeSH
- bisphenol A MeSH Browser
- bisphenol B MeSH Browser
- Endocrine Disruptors MeSH
- Estrogens, Non-Steroidal MeSH
- Phenols MeSH
- Testosterone MeSH
The increasing worldwide production of bisphenols has been associated to several human diseases, such as chronic respiratory and kidney diseases, diabetes, breast cancer, prostate cancer, behavioral troubles and reproductive disorders in both sexes. The aim of the present in vitro study was to evaluate the potential impact bisphenols A, B, S and F on the cell viability and testosterone release in TM3 Leydig cell line. Mice Leydig cells were cultured in the presence of different concentrations of bisphenols (0.04-50 µg.ml-1) during 24 h exposure. Quantification of the cell viability was assessed using the metabolic activity assay, while the level of testosterone in cell culture media was determined by enzyme-linked immunosorbent assay. Within the panel of substances under investigations, the higher experimental concentrations (10; 25 and 50 µg.ml-1) significantly (P<0.001) decreased Leydig cells viability, while the same doses of BPA and BPB also reduced testosterone production significantly (P<0.001). Taken together, the results of our study reported herein is a consistent whit the conclusion that higher experimental doses of bisphenols have a cytotoxic effect and could have a dose-dependent impact on testosterone production.
References provided by Crossref.org
Toxicity of bisphenol A and its replacements in the mice Leydig cells in vitro
Men´s reproductive alterations caused by bisphenol A and its analogues: a review
Introduction to DOK2 and its potential role in cancer
Endocrine disruptors and gut microbiome interactions
