We explored photoprotective strategies in a cryptophyte alga Rhodomonas salina. This cryptophytic alga represents phototrophs where chlorophyll a/c antennas in thylakoids are combined with additional light-harvesting system formed by phycobiliproteins in the chloroplast lumen. The fastest response to excessive irradiation is induction of non-photochemical quenching (NPQ). The maximal NPQ appears already after 20 s of excessive irradiation. This initial phase of NPQ is sensitive to Ca2+ channel inhibitor (diltiazem) and disappears, also, in the presence of non-actin, an ionophore for monovalent cations. The prolonged exposure to high light of R. salina cells causes photoinhibition of photosystem II (PSII) that can be further enhanced when Ca2+ fluxes are inhibited by diltiazem. The light-induced reduction in PSII photochemical activity is smaller when compared with immotile diatom Phaeodactylum tricornutum. We explain this as a result of their different photoprotective strategies. Besides the protective role of NPQ, the motile R. salina also minimizes high light exposure by increased cell velocity by almost 25% percent (25% from 82 to 104 μm/s). We suggest that motility of algal cells might have a photoprotective role at high light because algal cell rotation around longitudinal axes changes continual irradiation to periodically fluctuating light.

Institute of Microbiology Centre ALGATECH Czech Academy of Sciences Třeboň Czech Republic

Student of Faculty of Science University of South Bohemia Branišovská 31 370 05 Ceske Budejovice Czech Republic

See more in PubMed

PLoS One. 2010 Jun 08;5(6):e11000 PubMed

Plant Cell. 2011 Aug;23(8):2950-63 PubMed

Sci Rep. 2015 Sep 01;5:13679 PubMed

Biochim Biophys Acta. 2011 Jul;1807(7):841-6 PubMed

FEBS Lett. 2002 Jul 17;523(1-3):163-6 PubMed

Philos Trans R Soc Lond B Biol Sci. 2014 Mar 03;369(1640):20130221 PubMed

J Exp Bot. 2005 Jan;56(411):347-56 PubMed

Plant Physiol. 2006 Jun;141(2):391-6 PubMed

Plant Physiol. 1978 May;61(5):816-8 PubMed

Plant J. 2010 Jan;61(2):283-9 PubMed

Planta. 1997;203(3):265-74 PubMed

Nature. 2009 Nov 26;462(7272):518-21 PubMed

Sci Rep. 2016 Feb 18;6:21339 PubMed

Plant J. 2013 Nov;76(4):568-79 PubMed

Plant Physiol. 1998 Dec;118(4):1447-54 PubMed

J Am Chem Soc. 2013 Dec 11;135(49):18339-42 PubMed

Science. 2015 Aug 7;349(6248):647-50 PubMed

Photosynth Res. 2008 Oct-Dec;98(1-3):565-74 PubMed

Biochim Biophys Acta. 1956 Aug;21(2):234-44 PubMed

Plant Physiol. 2010 Dec;154(4):1905-20 PubMed

Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5 PubMed

Nat Chem Biol. 2015 Apr;11(4):287-91 PubMed

Science. 2008 May 9;320(5877):794-7 PubMed

Biochim Biophys Acta. 2015 Sep;1847(9):993-1003 PubMed

Physiol Plant. 2019 May;166(1):309-319 PubMed

Mol Plant. 2016 Aug 1;9(8):1183-1196 PubMed

Proc Natl Acad Sci U S A. 2014 May 20;111(20):7480-5 PubMed

PLoS One. 2012;7(1):e29700 PubMed

Photosynth Res. 2004;80(1-3):245-63 PubMed

Photosynth Res. 2011 Jan;107(1):103-15 PubMed

Photosynth Res. 2002 Oct;74(1):51-59 PubMed

Photosynth Res. 2000;65(2):141-54 PubMed

J Biol Chem. 2009 May 29;284(22):15255-66 PubMed

Photosynth Res. 2018 Mar;135(1-3):263-274 PubMed

Phys Chem Chem Phys. 2013 Aug 7;15(29):12253-61 PubMed

J Biol Chem. 2018 Apr 6;293(14):5018-5025 PubMed

Photosynth Res. 2008 Oct-Dec;98(1-3):589-608 PubMed

J Proteome Res. 2011 Dec 2;10(12):5338-53 PubMed

Trends Plant Sci. 2009 Apr;14(4):200-5 PubMed

Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12311-6 PubMed

Biophys J. 2012 Jun 20;102(12):2761-71 PubMed

Biochim Biophys Acta. 2012 Jan;1817(1):258-65 PubMed

Plant Physiol. 1999 Apr;119(4):1379-86 PubMed

Plant Physiol. 1994 Oct;106(2):763-770 PubMed

Biochemistry. 2006 Feb 7;45(5):1490-8 PubMed

Nature. 2012 Dec 6;492(7427):59-65 PubMed

J Exp Bot. 2005 Jan;56(411):365-73 PubMed

Biochim Biophys Acta. 2013 Mar;1827(3):294-302 PubMed

FEBS Lett. 2009 Feb 18;583(4):670-4 PubMed

Biochim Biophys Acta. 2012 Jan;1817(1):232-8 PubMed

Plant Cell Physiol. 2017 Dec 1;58(12):2217-2225 PubMed

Biochim Biophys Acta. 2015 Apr-May;1847(4-5):468-485 PubMed

Plant Cell Physiol. 2006 Jul;47(7):1010-6 PubMed

Physiol Plant. 2011 May;142(1):6-16 PubMed

Plant Physiol. 1987 May;84(1):178-81 PubMed

Biochim Biophys Acta. 2016 Jun;1857(6):840-7 PubMed

Front Plant Sci. 2016 Dec 16;7:1849 PubMed

Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):2213-8 PubMed

Plant Physiol. 2002 Jul;129(3):1398-406 PubMed

J Plant Physiol. 2015 Jan 1;172:13-32 PubMed

FEBS Lett. 2016 Apr;590(8):1076-85 PubMed

Biochim Biophys Acta. 2012 Jan;1817(1):167-81 PubMed

Nat Chem Biol. 2014 Jul;10(7):492-501 PubMed

New Phytol. 2010 Jul;187(1):23-43 PubMed

Biochim Biophys Acta. 2009 Jan;1787(1):3-14 PubMed

Mol Biol Cell. 2002 Sep;13(9):3303-13 PubMed

J Bioenerg Biomembr. 2002 Dec;34(6):455-64 PubMed

PLoS One. 2012;7(10):e47036 PubMed

J Exp Bot. 2018 Aug 14;69(18):4483-4493 PubMed

Biochim Biophys Acta. 1975 Jun 11;394(1):76-92 PubMed

Nat Plants. 2015 Nov 09;1:15168 PubMed

Photosynth Res. 1993 Aug;37(2):117-30 PubMed

Curr Opin Plant Biol. 2017 Jun;37:102-108 PubMed

Plant Cell. 1998 Jul;10(7):1121-34 PubMed

Plant J. 2010 Jun 1;62(6):948-59 PubMed

J Biol Chem. 2009 Sep 11;284(37):25343-52 PubMed

Nat Commun. 2016 May 24;7:11654 PubMed

J Biol Chem. 2016 Apr 1;291(14):7334-46 PubMed

Biochemistry. 2003 May 20;42(19):5802-8 PubMed

Biochim Biophys Acta. 2012 Jan;1817(1):209-17 PubMed

Biochim Biophys Acta. 2012 Jan;1817(1):247-57 PubMed

Nature. 2000 Jan 27;403(6768):391-5 PubMed

J Biol Chem. 2002 Jun 21;277(25):22750-8 PubMed

Plant Physiol. 2001 Apr;125(4):1558-66 PubMed

J Biol Chem. 2006 Jan 13;281(2):1145-51 PubMed

Nature. 2007 Nov 22;450(7169):575-8 PubMed

FEBS Lett. 2011 Jun 23;585(12):1941-5 PubMed

Biophys J. 2005 Dec;89(6):4310-9 PubMed

Biophys J. 2004 Apr;86(4):2342-9 PubMed

Front Plant Sci. 2016 Feb 09;7:115 PubMed

J Biol Chem. 2013 Aug 9;288(32):23529-42 PubMed

FEBS J. 2008 Mar;275(6):1069-79 PubMed

J Exp Bot. 2015 Oct;66(20):6461-70 PubMed

Science. 2013 Oct 4;342(6154):114-8 PubMed

Plant Physiol. 2007 Feb;143(2):629-38 PubMed

Plant Cell Environ. 2015 Oct;38(10):2035-47 PubMed

Plant Physiol. 1995 Jun;108(2):721-726 PubMed

Biochim Biophys Acta. 2000 Jun 1;1466(1-2):187-204 PubMed

Photosynth Res. 2007 Jun;92(3):275-87 PubMed

Front Plant Sci. 2016 Feb 29;7:218 PubMed

Photosynth Res. 1994 Sep;41(3):371-9 PubMed

Plant Physiol. 2000 Feb;122(2):583-96 PubMed

Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13516-21 PubMed

Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2988-2993 PubMed

J Exp Bot. 2005 Jan;56(411):395-406 PubMed

Photosynth Res. 2002;72(3):271-84 PubMed

Annu Rev Plant Biol. 2009;60:239-60 PubMed

Proc Jpn Acad Ser B Phys Biol Sci. 2016;92(9):387-411 PubMed

Biochim Biophys Acta. 2009 Jul;1787(7):929-38 PubMed

Science. 2009 Jul 24;325(5939):487-90 PubMed

Ann Bot. 2018 Dec 31;122(7):1263-1278 PubMed

J Photochem Photobiol B. 2011 Jul-Aug;104(1-2):377-85 PubMed

Science. 2002 Jun 28;296(5577):2395-8 PubMed

Biochem Soc Trans. 2018 Oct 19;46(5):1263-1277 PubMed

Nat Commun. 2014 Nov 13;5:5439 PubMed

Biol Chem. 2007 Sep;388(9):927-35 PubMed

New Phytol. 2009 Jan;181(2):275-294 PubMed

Trends Plant Sci. 2018 Aug;23(8):667-676 PubMed

J Biol Chem. 2000 Apr 28;275(17):12781-8 PubMed

Proc Natl Acad Sci U S A. 2010 May 11;107(19):8860-5 PubMed

Science. 2005 Jan 21;307(5708):433-6 PubMed

Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):14204-9 PubMed

Special issue dedicated to the memory of Ivan Šetlík