In this work, interactions of carboxylated core shell magnetic nanoparticles with polymyxin B sulfate were studied by connecting capillary electrophoresis with inductively coupled plasma mass spectrometry. The interaction was probed by affinity mode of capillary electrophoresis with 25 mM phosphate buffer at physiological pH. 54Fe, 56Fe, 57Fe, 34S, and 12C isotopes were used to monitor the migration of an electroosmotic flow marker and the interaction of the nanoparticles with polymyxin B. The analysis of interaction data showed two distinct interaction regions, one with low polymyxin B concentration, the second with high polymyxin B concentration. These regions differed in the strength of the interaction, 1.49 × 107 M-1 and 1.60 × 104 M-1, and in the stoichiometry of 0.7 and 3.5, respectively. These differences can be explained by the decrease of electrostatic repulsion between nanoparticles caused by polymyxin B. This is also in agreement with the nanoparticles peak shapes: sharp for low polymyxin B concentrations and broad for high polymyxin B concentrations.

Department of Analytical Chemistry Regional Centre of Advanced Technologies and Materials Faculty of Science Palacký University in Olomouc 17 listopadu 12 77146 Olomouc Czech Republic

Sorbonne Université CNRS Physico chimie des Électrolytes et Nanosystèmes Interfaciaux PHENIX F 75005 Paris France

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Characterization of Nanoparticles in Mixtures by Taylor Dispersion Analysis Hyphenated to Inductively Coupled Plasma Mass Spectrometry