CD Study of the G-Quadruplex Conformation
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Circular dichroism spectroscopy, DNA conformational transitions, Guanine quadruplex, Guanine-rich genomic sequences, Interpretation of CD spectra,
- MeSH
- Circular Dichroism MeSH
- X-Ray Diffraction MeSH
- DNA chemistry MeSH
- G-Quadruplexes * MeSH
- Nucleic Acid Conformation MeSH
- Magnetic Resonance Spectroscopy MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
Circular Dichroic (CD) spectroscopy is one of the most frequently used methods for guanine quadruplex studies and in general for studies of conformational properties of nucleic acids. The reason is its high sensitivity to even slight changes in mutual orientation of absorbing bases of DNA. CD can reveal formation of particular structural DNA arrangements and can be used to search for the conditions stabilizing the structures, to follow the transitions between various structural states, to explore kinetics of their appearance, to determine thermodynamic parameters, and also to detect formation of higher order structures. CD spectroscopy is an important complementary technique to NMR spectroscopy and X-ray diffraction in quadruplex studies due to its sensitivity, easy manipulation of studied samples, and relative inexpensiveness. In this part, we present the protocol for the use of CD spectroscopy in the study of guanine quadruplexes, together with practical advice and cautions about various, particularly interpretation, difficulties.
References provided by Crossref.org
DNA i-motif formation at neutral pH is driven by kinetic partitioning
Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip
Diversity of Parallel Guanine Quadruplexes Induced by Guanine Substitutions
