Evaluation of TD test for analysis of persistence or tolerance in clinical isolates of Staphylococcus aureus
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
31472196
DOI
10.1016/j.mimet.2019.105705
PII: S0167-7012(19)30561-5
Knihovny.cz E-resources
- Keywords
- Evaluation, Persistence, Staphylococcus aureus, TD test, Tolerance,
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Cystic Fibrosis microbiology MeSH
- Disk Diffusion Antimicrobial Tests methods MeSH
- Glucose metabolism MeSH
- Respiratory Tract Infections microbiology MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus aureus drug effects isolation & purification MeSH
- Check Tag
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Glucose MeSH
Besides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance. The main purpose of this study was to evaluate the suitability of newly developed Tolerance Disk Test (TDtest) (Gefen et al., 2017) to detect persistent or tolerant bacterial cells in clinical isolates of Staphylococcus aureus. The principle of the test is to resuscitate the subpopulation of persistent or tolerant bacterial cells following a disk diffusion test by glucose. Results of the TDtest were evaluated using time killing experiments for three pairs of consecutive S. aureus isolates from lower respiratory airway samples of three cystic fibrosis patients with chronic staphylococcal infections. TDtest enabled semi-quantitative detection of persistent or tolerant bacterial populations in all analyzed isolates for oxacillin, vancomycin, and ciprofloxacin to which isolates studied were susceptible. Therefore, TDtest is a promising method for rapidly determining persistence/tolerance in clinical isolates of S. aureus.
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