Enantioselective potential of teicoplanin- and vancomycin-based superficially porous particles-packed columns for supercritical fluid chromatography
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
31727354
DOI
10.1016/j.chroma.2019.460687
PII: S0021-9673(19)31116-1
Knihovny.cz E-resources
- Keywords
- Chiral stationary phase, Enantioseparation, Supercritical fluid chromatography, Superficially porous particles, Teicoplanin, Vancomycin,
- MeSH
- Alkaloids chemistry MeSH
- Phytoalexins MeSH
- Ketamine chemistry MeSH
- Trifluoroacetic Acid chemistry MeSH
- Porosity MeSH
- Solvents chemistry MeSH
- Sesquiterpenes chemistry MeSH
- Stereoisomerism MeSH
- Chromatography, Supercritical Fluid methods MeSH
- Teicoplanin chemistry MeSH
- Vancomycin chemistry MeSH
- Hydrogen Bonding MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Alkaloids MeSH
- cathinone MeSH Browser
- Phytoalexins MeSH
- Ketamine MeSH
- Trifluoroacetic Acid MeSH
- Solvents MeSH
- Sesquiterpenes MeSH
- Teicoplanin MeSH
- Vancomycin MeSH
Application of the superficially porous particles (SPPs) grafted with chiral selectors can substantially improve resolution in chromatographic techniques. In this work, we carried out a deeper study on supercritical fluid chromatography systems with 2.7 µm SPPs bonded with teicoplanin and vancomycin. Fast separations of the majority of enantiomers of phytoalexins, substituted tryptophans, and ketamine derivatives, as representatives of important biologically active and structurally diverse chiral compounds have been achieved. The chromatographic behavior of the structurally different analytes served to characterize these separation systems. The influence of separation conditions, namely mobile phase composition, i.e. type of co-solvent and additive on retention, enantioselective resolution and enantioselectivity was examined. The success rate of baseline and partial separations in individual groups of compounds differed with the chiral stationary phase and also with mobile phase composition. The best, baseline separations for the phytoalexins were achieved on the TeicoShell column using methanol as a co-solvent and trifluoroacetic acid as an additive if used. Mostly partial separations were achieved on the vancomycin-based column for all groups of analytes. Complementary separation behavior of these CSPs was confirmed for the majority of the chiral compounds examined in this work.
Department of Chemistry and Biochemistry University of Texas at Arlington Arlington TX United States
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