Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and "ischemic" conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.

Department of Biomedical Technology Faculty of Biomedical Engineering Czech Technical University Prague Sitna sq 3105 27201 Kladno Czech Republic

Department of Health Care Disciplines and Population Protection Faculty of Biomedical Engineering Czech Technical University Prague Sportovcu st 2311 27201 Kladno Czech Republic

Department of Human and Animal Physiology Lomonosov Moscow State University Leninskiye gory 1 12 119234 Moscow Russia

Department of Normal Physiology Pavlov 1st State Medical University of Saint Petersburg Lev Tolstoy st 6 8 197022 St Petersburg Russia

Department of Physiology Institute of Medicine of Pitirim Sorokin Syktyvkar State University Starovskii st 55 167001 Syktyvkar Russia

Institute of Experimental Medicine Almazov National Medical Research Centre Akkuratov st 2 197341 St Petersburg Russia

Institute of Physiology Federal Research Centre Komi Science Centre Ural Branch of Russian Academy of Sciences Pervomayskaya st 50 167982 Syktyvkar Russia

Instituto de Fisiología Facultad de Ciencias Médicas Universidad Nacional de Cuyo Av Libertador 80 Centro Universitario CP 5500 Mendoza Argentina

Instituto de Medicina y Biología Experimental de Cuyo CP 5500 Mendoza Argentina

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