Fabrication of nanocomposite biofilms with enhanced mechanical and antibacterial properties was successfully achieved from hyaluronan (HA) and partially deacetylated chitin nanowhiskers (ChNWs) by a casting-evaporation method. The hydrolysis process of chitin showed an important role in the dimensions, stability, and the crystallinity of extracted ChNWs in a time-dependent manner. The volume fraction of ChNWs nanofiller varying from (0.001 to 0.5) exhibited a great influence on the mechanical properties of the biofilms (young modulus, strength) was enhanced by the high load-bearing capacity of NWs compared with net HA film. The antibacterial activity of the nanocomposite biofilms exhibited significant bactericidal activity against different types of bacteria (-/+ gram). HA/ChNWs Nanocomposite biofilms did not show any toxicity against normal human dermal fibroblasts (NHDF) and human primary osteogenic sarcoma (Saos-2) cell lines. The new biofilms with unique properties like edibleness, environmental friendliness, high mechanical properties, antibacterial performance, and non-cytotoxicity that could be used in skin tissue regenerations, and drug delivery applications.

CEITEC Central European Institute of Technology Brno University of Technology Purkynova 656 123 Brno 61200 Czechia

CEITEC Central European Institute of Technology Brno University of Technology Purkynova 656 123 Brno 61200 Czechia; SCITEG a s Brno Czechia; Department of Pretreatment and Finishing of Cellulosic based Textiles Textile Industries Research Division National Research Centre 33 EL Buhouth St Dokki Giza 12622 Egypt

CEITEC Central European Institute of Technology Brno University of Technology Purkynova 656 123 Brno 61200 Czechia; SCITEG a s Brno Czechia; Institute of Materials Chemistry Facility of Chemistry Brno University of Technology Purkyňova 464 118 Brno 61200 Czechia

Department of Medical Chemistry and Biochemistry Faculty of Medicine and Dentistry Palacky University Olomouc Czechia

Department of Physical Electronics Masaryk University Kotlářská 267 2 Brno 611 37 Czechia

Institute of Macromolecular Chemistry of the Czech Academy of Sciences Heyrovskeho Nam 2 162 06 Prague 6 Czechia

References provided by Crossref.org

Self-Assembled Hydrogel Membranes with Structurally Tunable Mechanical and Biological Properties

Marine Biomaterials: Hyaluronan

Magnetic Superporous Poly(2-hydroxyethyl methacrylate) Hydrogel Scaffolds for Bone Tissue Engineering