Long-term high intake of 9-PAHPA or 9-OAHPA increases basal metabolism and insulin sensitivity but disrupts liver homeostasis in healthy mice
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32179409
DOI
10.1016/j.jnutbio.2020.108361
PII: S0955-2863(19)30954-4
Knihovny.cz E-resources
- Keywords
- FAHFA, Inflammation, Insulin sensitivity, Liver fibrosis, Liver steatosis, Mice,
- MeSH
- Basal Metabolism drug effects MeSH
- Adipose Tissue, White drug effects metabolism MeSH
- Energy Metabolism MeSH
- Glucose Tolerance Test MeSH
- Homeostasis MeSH
- Insulin metabolism MeSH
- Insulin Resistance * MeSH
- Liver Cirrhosis metabolism MeSH
- Liver metabolism MeSH
- Blood Glucose analysis MeSH
- Fatty Acids administration & dosage adverse effects pharmacology MeSH
- Lipid Metabolism MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Body Weight drug effects MeSH
- Inflammation metabolism MeSH
- Fatty Liver metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Insulin MeSH
- Blood Glucose MeSH
- Fatty Acids MeSH
Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are a new family of endogenous lipids recently discovered. Several studies reported that some FAHFAs have antidiabetic and anti-inflammatory effects. The objective of this study was to explore the impact of two FAHFAs, 9-PAHPA or 9-OAHPA, on the metabolism of mice. C57Bl/6J male mice, 6 weeks old, were divided into 3 groups of 10 mice each. One group received a control diet and the two others groups received the control diet supplemented with 9-PAHPA or 9-OAHPA for 12 weeks. Mouse weight and body composition were monitored throughout the study. Some days before euthanasia, energy expenditure, glucose tolerance and insulin sensitivity were also determined. After sacrifice, blood and organs were collected for relevant molecular, biochemical and histological analyses. Although high intake of 9-PAHPA or 9-OAHPA increased basal metabolism, it had no direct effect on body weight. Interestingly, the 9-PAHPA or 9-OAHPA intake increased insulin sensitivity but without modifying glucose tolerance. Nevertheless, 9-PAHPA intake induced a loss of glucose-stimulated insulin secretion. Surprisingly, both studied FAHFAs induced hepatic steatosis and fibrosis in some mice, which were more marked with 9-PAHPA. Finally, a slight remodeling of white adipose tissue was also observed with 9-PAHPA intake. In conclusion, the long-term high intake of 9-PAHPA or 9-OAHPA increased basal metabolism and insulin sensitivity in healthy mice. However, this effect, highly likely beneficial in a diabetic state, was accompanied by manifest liver damage in certain mice that should deserve special attention in both healthy and pathological studies.
Aix Marseille Univ INSERM INRAE C2VN Marseille France
DMEM INRAE Univ Montpellier Montpellier France
IBMM Univ Montpellier CNRS ENSCM Montpellier France
INSERM U1194 Network of Experimental Histology BioCampus CNRS UMS3426 Montpellier France
LNC UMR1231 INSERM Univ Bourgogne Franche Comté Agrosup Dijon LipSTIC LabEx Dijon France
References provided by Crossref.org
