Molecular identification of Sarcocystis lutrae (Apicomplexa: Sarcocystidae) from the raccoon dog, Nyctereutes procyonoides, and the common raccoon, Procyon lotor, in the Czech Republic
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články
PubMed
32375886
PubMed Central
PMC7204025
DOI
10.1186/s13071-020-04108-z
PII: 10.1186/s13071-020-04108-z
Knihovny.cz E-zdroje
- Klíčová slova
- Common raccoon, Czech Republic, Molecular characterization, Raccoon dog, Sarcocystis lutrae, Wildlife,
- MeSH
- fylogeneze MeSH
- molekulární patologie MeSH
- mývalové parazitologie MeSH
- protozoální geny MeSH
- protozoální infekce MeSH
- psík mývalovitý parazitologie MeSH
- RNA ribozomální 18S genetika MeSH
- RNA ribozomální 28S genetika MeSH
- Sarcocystidae klasifikace MeSH
- Sarcocystis klasifikace genetika izolace a purifikace MeSH
- stadia vývoje MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- RNA ribozomální 18S MeSH
- RNA ribozomální 28S MeSH
BACKGROUND: Apicomplexan parasites of the genus Sarcocystis have an obligate two-host life-cycle and comprise about 200 species, which infect different cold- and warm-blooded hosts, including humans. Recently, morphological and molecular studies of sarcocysts in broadly spread carnivore hosts have been on the rise. The description of muscular tissues infection by Sarcocystis in the raccoon dog and the common raccoon from the Czech Republic is herein presented. METHODS: During January-August 2019, 15 raccoon dogs and 1 common raccoon were examined from 5 districts (Česká Lípa, Liberec, Mladá Boleslav, Trutnov and Ústí nad Labem) of the Czech Republic. Muscle parts (diaphragm, forearm, hind-limb, tongue and heart) were examined in wet preparations under compression by light microscopy. After finding Sarcocystis sp., morphological characteristics and molecular analyses of 18S rRNA, 28S rRNA, ITS1 and cox1 loci were used to identify the species. RESULTS: Sarcocysts were detected and identified in 1 out of 15 raccoon dogs and in the single common raccoon. Preferential infection sites were diaphragm and tongue, followed by forearm and hind limb. To our knowledge, this is the first identification of microscopic sarcocysts by multi-locus genetic analysis from both host species. Molecular analyses revealed 100% similarity at 18S rRNA, 28S rRNA, and cox1 genes with S. lutrae for both hosts and 98-100% identity at the ITS1 region of the isolate from the common raccoon. CONCLUSIONS: Both widely distributed non-indigenous wild carnivores represent new intermediate host records for S. lutrae and the first report of this parasite in a member of the family Procyonidae, but still with an unknown natural definitive host. Molecular data revealed that this parasite species appears more closely related to the Sarcocystis spp. using raptorial birds as definitive hosts. Therefore, further studies aimed at its identification, including the complete life-cycle, remain necessary.
Zobrazit více v PubMed
Dubey JP, Calero-Bernal R, Rosenthal BM, Speer CA, Fayer R. Sarcocystosis of animals and humans. Boca Raton: CRC Press; 2016.
Gjerde B, Josefsen TD. Molecular characterisation of Sarcocystis lutrae n. sp. and Toxoplasma gondii from the musculature of two Eurasian otters (Lutra lutra) in Norway. Parasitol Res. 2015;114:873–886. doi: 10.1007/s00436-014-4251-8. PubMed DOI
Lepore T, Bartley PM, Chianini F, Macrae AI, Innes EA, Katzer F. Molecular detection of Sarcocystis lutrae in the European badger (Meles meles) in Scotland. Parasitology. 2017;144:1426–1432. doi: 10.1017/S0031182017000762. PubMed DOI
Kirillova V, Prakas P, Calero-Bernal R, Gavarāne I, Fernández-García JL, Martínez-González M, et al. Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain. Parasit Vectors. 2018;11:173. doi: 10.1186/s13071-018-2694-y. PubMed DOI PMC
Máca O. Molecular identification of Sarcocystis lutrae in the European otter (Lutra lutra) and the European badger (Meles meles) from the Czech Republic. Parasitol Res. 2018;117:943–945. doi: 10.1007/s00436-018-5793-y. PubMed DOI
Prakas P, Strazdaitė-Žielienė Ž, Rudaitytė-Lukošienė E, Servienė E, Butkauskas D. Molecular identification of Sarcocystis lutrae (Apicomplexa: Sarcocystidae) in muscles of five species of the family Mustelidae. Parasitol Res. 2018;117:1989–1993. doi: 10.1007/s00436-018-5880-0. PubMed DOI
Beltrán-Beck B, García FJ, Gortázar C. Raccoons in Europe: disease hazards due to the establishment of an invasive species. Eur J Wildl Res. 2011;58:5–15. doi: 10.1007/s10344-011-0600-4. DOI
Oksanen A, Siles M, Karamon J, Possenti A, Conraths F, Romig T, et al. The geographical distribution and prevalence of Echinococcus multilocularis in animals in the European Union and adjacent countries: a systematic review and meta-analysis. Parasit Vectors. 2016;9:519. doi: 10.1186/s13071-016-1746-4. PubMed DOI PMC
Kärssin A, Häkkinen L, Niin E, Peik K, Vilem A, Jokelainen P, et al. Trichinella spp. biomass has increased in raccoon dogs (Nyctereutes procyonoides) and red foxes (Vulpes vulpes) in Estonia. Parasit Vectors. 2017;10:609. doi: 10.1186/s13071-017-2571-0. PubMed DOI PMC
Cybulska A, Skopek R, Kornacka A, Popiołek M, Piróg A, Laskowski Z, et al. First detection of Trichinella pseudospiralis infection in raccoon (Procyon lotor) in central Europe. Vet Parasitol. 2018;254:114–119. doi: 10.1016/j.vetpar.2018.03.007. PubMed DOI
Dubey JP, Howe DK, Furr M, Saville WJ, Marsh AE, Reed SM, et al. An update on Sarcocystis neurona infections in animals and equine protozoal myeloencephalitis (EPM) Vet Parasitol. 2015;209:1–42. doi: 10.1016/j.vetpar.2015.01.026. PubMed DOI PMC
Snyder DE, Sanderson GC, Toivio-Kinnucan M, Blagburn BL. Sarcocystis kirkpatricki n. sp. (Apicomplexa: Sarcocystidae) in muscles of raccoons (Procyon lotor) from Illinois. J Parasitol. 1990;76:495–500. doi: 10.2307/3282827. PubMed DOI
Stolte M, Odening K, Walter G, Bockhardt I. The raccoon as intermediate host of three Sarcocystis species in Europe. Comp Parasitol. 1996;63:145–149.
Kubo M, Okano T, Ito K, Tsubota T, Sakai H, Yanai T. Muscular sarcocystosis in wild carnivores in Honshu. Japan. Parasitol Res. 2009;106:213–219. doi: 10.1007/s00436-009-1653-0. PubMed DOI
Dubey JP, Sykes JE, Shelton GD, Sharp N, Verma SK, Calero-Bernal R, et al. Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) associated with severe myositis and hepatitis in the domestic dog (Canis familiaris) J Eukaryot Microbiol. 2015;62:307–317. doi: 10.1111/jeu.12182. PubMed DOI PMC
Mugridge NB, Morrison DA, Johnson AM, Luton K, Dubey JP, Votýpka J, et al. Phylogenetic relationships of the genus Frenkelia: a review of its history and new knowledge gained from comparison of large subunit ribosomal ribonucleic acid gene sequences. Int J Parasitol. 1999;29:957–972. doi: 10.1016/S0020-7519(99)00062-4. PubMed DOI
Gjerde B. Molecular characterisation of Sarcocystis rileyi from a common eider (Somateria mollissima) in Norway. Parasitol Res. 2014;113:3501–3509. doi: 10.1007/s00436-014-4062-y. PubMed DOI
Rosenthal BM, Dunams DB, Pritt B. Restricted genetic diversity in the ubiquitous cattle parasite, Sarcocystis cruzi . Infect Genet Evol. 2008;8:588–592. doi: 10.1016/j.meegid.2008.04.004. PubMed DOI
Gjerde B. Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol. 2013;43:579–591. doi: 10.1016/j.ijpara.2013.02.004. PubMed DOI
Gjerde B. Sarcocystis species in red deer revisited: with a re-description of two known species as Sarcocystis elongata n. sp. and Sarcocystis truncata n. sp. based on mitochondrial cox1 sequences. Parasitology. 2014;141:441–452. doi: 10.1017/S0031182013001819. PubMed DOI
Pavlásek I, Máca O. Morphological and molecular identification of Sarcocystis arctica sarcocysts in three red foxes (Vulpes vulpes) from the Czech Republic. Parasitol Int. 2017;66:603–605. doi: 10.1016/j.parint.2017.05.003. PubMed DOI
Gjerde B, Vikøren T, Hamnes IS. Molecular identification of Sarcocystis halieti n. sp., Sarcocystis lari and Sarcocystis truncata in the intestine of a white-tailed sea eagle (Haliaeetus albicilla) in Norway. Int J Parasitol Parasites Wildl. 2017;7:1–11. doi: 10.1016/j.ijppaw.2017.12.001. PubMed DOI PMC