Characterization and in vitro assembly of tick-borne encephalitis virus C protein
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- C protein, DENV, TBEV, assembly, flavivirus, protein-nucleic acid interaction,
- MeSH
- Circular Dichroism MeSH
- Chromatography, Gel MeSH
- Hydrogen-Ion Concentration MeSH
- Magnetic Resonance Spectroscopy MeSH
- Nucleocapsid chemistry metabolism MeSH
- Recombinant Proteins chemistry genetics isolation & purification MeSH
- Viral Proteins chemistry genetics isolation & purification metabolism MeSH
- Dengue Virus chemistry MeSH
- Encephalitis Viruses, Tick-Borne chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Recombinant Proteins MeSH
- Viral Proteins MeSH
Tick-borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical-chemical approaches, such as size-exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid-like structure with that of dengue C protein.
Department of Biotechnology University of Chemistry and Technology Prague Prague Czech Republic
Faculty of Science University of South Bohemia České Budějovice Czech Republic
Faculty of Tropical AgriSciences Czech University of Life Sciences Prague Prague Czech Republic
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
NMR Laboratory University of Chemistry and Technology Prague Prague Czech Republic
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