assembly
Dotaz
Zobrazit nápovědu
Pediatric allergy and immunology. No. 9, ISSN 0906-5784 Supplementum Vol. 7
122 s. : il. ; 30 cm
Archives of physical medicine and rehabilitation, ISSN 0003-9993
svazky ; 28 cm
Allergy, ISSN 0108-1675 vol. 54, suppl. 51, 1999
48 s. : il., tab. ; 30 cm
svazky
- MeSH
- celosvětové zdraví MeSH
- kongresy jako téma MeSH
- Světová zdravotnická organizace MeSH
- Publikační typ
- kongresy MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- veřejné zdravotnictví
- veřejné zdravotnictví
Dysfunction of chromatin assembly factor 1 in FASCIATA mutants (fas) of Arabidopsis thaliana results in progressive loss of telomeric DNA. Although replicative telomere shortening is typically associated with incomplete resynthesis of their ends by telomerase, no change in telomerase activity could be detected in vitro in extracts from fas mutants. Besides a possible telomerase malfunction, the telomere shortening in fas mutants could presumably be due to problems with conventional replication of telomeres. To distinguish between the possible contribution of suboptimal function of telomerase in fas mutants under in vivo conditions and problems in conventional telomere replication, we crossed fas and tert (telomerase reverse transcriptase) knockout mutants and analyzed telomere shortening in segregated fas mutants, tert mutants, and double fas tert mutants in parallel. We demonstrate that fas tert knockouts show greater replicative telomere shortening than that observed even in the complete absence of telomerase (tert mutants). While the effect of tert and fas mutations on telomere lengths in double mutants is additive, manifestations of telomere dysfunction in double fas tert mutants (frequency of anaphase bridges, onset of chromosome end fusions, and common involvement of 45S rDNA in chromosome fusion sites) are similar to those in tert mutants. We conclude that in addition to possible impairment of telomerase action, a further mechanism contributes to telomere shortening in fas mutants.
- MeSH
- Arabidopsis enzymologie genetika metabolismus MeSH
- chromozomy rostlin genetika metabolismus MeSH
- faktor 1 pro uspořádání chromatinu genetika metabolismus MeSH
- mutace * MeSH
- proteiny huseníčku genetika metabolismus MeSH
- telomerasa genetika metabolismus MeSH
- telomery genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
