Three neurosteroids as well as GABAergic drugs do not convert immediate postictal potentiation to depression in immature rats
Language English Country Switzerland Media print-electronic
Document type Comparative Study, Journal Article
Grant support
CZ_02.1.01/0.0/0.0/16_025/0007444
Ministerstvo Průmyslu a Obchodu (CZ)
18-09296S
Grantová Agentura České republiky (CZ)
PubMed
32515003
DOI
10.1007/s43440-020-00113-2
PII: 10.1007/s43440-020-00113-2
Knihovny.cz E-resources
- Keywords
- Cortical afterdischarges, Gabaergic drugs, Immature rats, Neurosteroids, Postictal potentiation,
- MeSH
- Anticonvulsants pharmacology MeSH
- Time Factors MeSH
- Electrodes, Implanted MeSH
- Rats MeSH
- Midazolam pharmacology MeSH
- Disease Models, Animal MeSH
- Muscimol pharmacology MeSH
- Neurosteroids pharmacology MeSH
- Rats, Wistar MeSH
- Receptors, GABA-A drug effects metabolism MeSH
- Seizures drug therapy MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Anticonvulsants MeSH
- Midazolam MeSH
- Muscimol MeSH
- Neurosteroids MeSH
- Receptors, GABA-A MeSH
BACKGROUND: Postictal potentiation presented immediately after cortical seizures in immature rats might be due to imbalance between excitation and inhibition. The aim of the present study was to determine whether augmentation of inhibition mediated by GABAA receptors could also suppress the postictal potentiation. METHODS: Twelve-day old rats with implanted electrodes were used in our study. Five drugs were tested: the agonist muscimol, the positive modulator midazolam and three neurosteroids affecting GABAA receptors-allopregnanolone, pregnanolone sulphate and pregnanolone glutamate. RESULTS: None of the five drugs was able to suppress potentiation appearing immediately after cortical epileptic afterdischarges, but all of them exhibited delayed anticonvulsant action 10 (in the case of midazolam and muscimol) or 20 min (all three steroids) after cortical seizures. CONCLUSION: Our results support a role of GABA in augmentation of cortical after discharges after longer intervals, whereas immediate postictal potentiation is not affected by GABAergic drugs. Due to similar effect with GABAergic drugs, the main mechanism of action of the three steroids tested is potentiation of GABAergic inhibition.
Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Prague Czech Republic
Institute of Physiology Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
Epilepsy Research in the Institute of Physiology of the Czech Academy of Sciences in Prague
