The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 hours following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 hours after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-hours following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.

Department of Animal Husbandry Sciences Faculty of Agriculture University of South Bohemia in Ceske Budejovice Studentska 1668 37005 Ceske Budejovice Czech Republic

Department of Animal Morphology Physiology and Genetics Faculty of AgriSciences Mendel University in Brno Zemedelska 1 613 00 Brno Czech Republic

Department of Animal Nutrition and Forage Production Faculty of AgriSciences Mendel University in Brno Zemedelska 1 613 00 Brno Czech Republic

Department of Animal Origin Food and Gastronomic Sciences Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Palackeho tr 1 612 42 Brno Czech Republic

Department of Immunology Veterinary Research Institute Hudcova 70 621 00 Brno Czech Republic

Department of Life Science and Bioinformatics Assam University Silchar 788 011 India

Department of Pharmacology School of Medicine Ajou University Suwon 16499 Korea

Department of Veterinary Sciences Faculty of Agrobiology and Food Resources Slovak University of Agriculture in Nitra Trieda A Hlinku 2 949 76 Nitra Slovakia

Hokkaido Research Station National Institute of Animal Health National Agriculture and Food Research Organization 4 Hitsujigaoka Toyohira Sapporo Hokkaido 062 0045 Japan

Laboratory of Biochemistry Faculty of Veterinary Medicine Hokkaido University Sapporo Hokkaido 060 0818 Japan

NPPC Research Institute for Animal Production Hlohovecka 2 951 41 Luzianky Slovakia

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Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland

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