Redox and Epigenetics in Human Pluripotent Stem Cells Differentiation
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32567336
DOI
10.1089/ars.2019.7983
Knihovny.cz E-resources
- Keywords
- DNA damage, histone variant macroH2A1, induced pluripotent stem cells (iPSCs), oxidative stress,
- MeSH
- Cell Differentiation * genetics MeSH
- Cell Self Renewal MeSH
- Epigenesis, Genetic * MeSH
- Induced Pluripotent Stem Cells cytology metabolism MeSH
- Humans MeSH
- DNA Methylation MeSH
- Mitochondria genetics metabolism MeSH
- Cell Transformation, Neoplastic genetics metabolism MeSH
- Genomic Instability MeSH
- Oxidation-Reduction * MeSH
- Oxidative Stress MeSH
- Oxidative Phosphorylation MeSH
- Pluripotent Stem Cells cytology metabolism MeSH
- Cellular Reprogramming genetics MeSH
- Regenerative Medicine MeSH
- Stem Cell Transplantation MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Significance: Since their discovery, induced pluripotent stem cells (iPSCs) had generated considerable interest in the scientific community for their great potential in regenerative medicine, disease modeling, and cell-based therapeutic approach, due to their unique characteristics of self-renewal and pluripotency. Recent Advances: Technological advances in iPSC genome-wide epigenetic profiling led to the elucidation of the epigenetic control of cellular identity during nuclear reprogramming. Moreover, iPSC physiology and metabolism are tightly regulated by oxidation-reduction events that mainly occur during the respiratory chain. In theory, iPSC-derived differentiated cells would be ideal for stem cell transplantation as autologous cells from donors, as the risks of rejection are minimal. Critical Issues: However, iPSCs experience high oxidative stress that, in turn, confers a high risk of increased genomic instability, which is most often linked to DNA repair deficiencies. Genomic instability has to be assessed before iPSCs can be used in therapeutic designs. Future Directions: This review will particularly focus on the links between redox balance and epigenetic modifications-in particular based on the histone variant macroH2A1-that determine DNA damage response in iPSCs and derived differentiated cells, and that might be exploited to decrease the teratogenic potential on iPSC transplantation. Antioxid. Redox Signal. 34, 335-349.
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic
Faculty of Informatics Centre for Biomedical Image Analysis Masaryk University Brno Czech Republic
International Clinical Research Center St' Anne's University Hospital Brno Czech Republic
References provided by Crossref.org
Gene Editing and Human iPSCs in Cardiovascular and Metabolic Diseases
Deficiency of histone variant macroH2A1.1 is associated with sexually dimorphic obesity in mice
