Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
32747247
DOI
10.1016/j.carbpol.2020.116588
PII: S0144-8617(20)30762-1
Knihovny.cz E-resources
- Keywords
- Aggregation, Chlorella, Immunomodulatory activity, Rhamnans, Structure, Xylogalactofuranan,
- MeSH
- B-Lymphocytes drug effects immunology pathology MeSH
- Antigens, CD genetics immunology MeSH
- Chlorophyta chemistry MeSH
- Immunologic Factors chemistry isolation & purification pharmacology MeSH
- Interferon-gamma genetics immunology MeSH
- Interleukin-2 genetics immunology MeSH
- Interleukin-4 genetics immunology MeSH
- Lipopolysaccharides antagonists & inhibitors pharmacology MeSH
- Melanoma immunology pathology MeSH
- Methylation MeSH
- Molecular Weight MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Skin Neoplasms immunology pathology MeSH
- Polysaccharides chemistry isolation & purification pharmacology MeSH
- Primary Cell Culture MeSH
- Gene Expression Regulation drug effects MeSH
- Plant Extracts chemistry MeSH
- Solubility MeSH
- Carbohydrate Sequence MeSH
- T-Lymphocytes drug effects immunology pathology MeSH
- Tumor Necrosis Factor-alpha genetics immunology MeSH
- Water MeSH
- Xylose chemistry isolation & purification MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens, CD MeSH
- Il4 protein, mouse MeSH Browser
- Immunologic Factors MeSH
- Interferon-gamma MeSH
- Interleukin-2 MeSH
- Interleukin-4 MeSH
- Lipopolysaccharides MeSH
- Polysaccharides MeSH
- Plant Extracts MeSH
- Tumor Necrosis Factor-alpha MeSH
- Water MeSH
- Xylose MeSH
Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 × 104g mol-1) was defined mainly as branched O-2-β-xylo-(1→3)-β-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 × 105 and 9.84 × 104g mol-1) were complex polysaccharides containing α-l-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-α production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.
Czech Academy of Sciences Institute of Microbiology Vídeňská 1083 142 20 Prague 4 Krč Czech Republic
Institute of Organic Chemistry and Biochemistry AS CR Flemingovo sq 2 166 28 Prague 6 Czech Republic
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