Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
32747247
DOI
10.1016/j.carbpol.2020.116588
PII: S0144-8617(20)30762-1
Knihovny.cz E-zdroje
- Klíčová slova
- Aggregation, Chlorella, Immunomodulatory activity, Rhamnans, Structure, Xylogalactofuranan,
- MeSH
- B-lymfocyty účinky léků imunologie patologie MeSH
- CD antigeny genetika imunologie MeSH
- Chlorophyta chemie MeSH
- imunologické faktory chemie izolace a purifikace farmakologie MeSH
- interferon gama genetika imunologie MeSH
- interleukin-2 genetika imunologie MeSH
- interleukin-4 genetika imunologie MeSH
- lipopolysacharidy antagonisté a inhibitory farmakologie MeSH
- melanom imunologie patologie MeSH
- metylace MeSH
- molekulová hmotnost MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- nádory kůže imunologie patologie MeSH
- polysacharidy chemie izolace a purifikace farmakologie MeSH
- primární buněčná kultura MeSH
- regulace genové exprese účinky léků MeSH
- rostlinné extrakty chemie MeSH
- rozpustnost MeSH
- sacharidové sekvence MeSH
- T-lymfocyty účinky léků imunologie patologie MeSH
- TNF-alfa genetika imunologie MeSH
- voda MeSH
- xylosa chemie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- CD antigeny MeSH
- Il4 protein, mouse MeSH Prohlížeč
- imunologické faktory MeSH
- interferon gama MeSH
- interleukin-2 MeSH
- interleukin-4 MeSH
- lipopolysacharidy MeSH
- polysacharidy MeSH
- rostlinné extrakty MeSH
- TNF-alfa MeSH
- voda MeSH
- xylosa MeSH
Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 × 104g mol-1) was defined mainly as branched O-2-β-xylo-(1→3)-β-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 × 105 and 9.84 × 104g mol-1) were complex polysaccharides containing α-l-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-α production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.
Czech Academy of Sciences Institute of Microbiology Vídeňská 1083 142 20 Prague 4 Krč Czech Republic
Institute of Organic Chemistry and Biochemistry AS CR Flemingovo sq 2 166 28 Prague 6 Czech Republic
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