In vitro evaluation of a novel nanostructured Ti-36Nb-6Ta alloy for orthopedic applications
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32752935
DOI
10.2217/nnm-2020-0123
Knihovny.cz E-resources
- Keywords
- adhesion, anodization, beta titanium alloy, biocompatibility, hMSC, mechanical properties, nanotubes, osteogenic differentiation, proliferation,
- MeSH
- Cell Adhesion MeSH
- Humans MeSH
- Microscopy, Electron, Scanning MeSH
- Nanotubes * MeSH
- Osteoblasts MeSH
- Osteogenesis MeSH
- Surface Properties MeSH
- Cell Proliferation MeSH
- Alloys MeSH
- Titanium * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alloys MeSH
- Titanium * MeSH
Aim: To evaluate the impact of a nanostructured surface created on β-titanium alloy, Ti-36Nb-6Ta, on the growth and differentiation of human mesenchymal stem cells. Materials & methods: The nanotubes, with average diameters 18, 36 and 46 nm, were prepared by anodic oxidation. Morphology, hydrophilicity and mechanical properties of the nanotube layers were characterized. The biocompatibility and osteogenic potential of the nanostructured surfaces were established using various in vitro assays, scanning electron microscopy and confocal microscopy. Results: The nanotubes lowered elastic modulus close to that of bone, positively influenced cell adhesion, improved ALP activity, synthesis of type I collagen and osteocalcin expression, but diminished early cell proliferation. Conclusion: Nanostructured Ti-36Nb-6Ta with nanotube diameters 36 nm was the most promising material for bone implantation.
References provided by Crossref.org
Human cells with osteogenic potential in bone tissue research
