Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-ε-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.

Central European Institute of Technology Brno University of Technology Purkynova 656 123 616 00 Brno Czech Republic

Department of Biophysics 2nd Faculty of Medicine Charles University 5 Uvalu 84 150 06 Prague Czech Republic

Institute of Experimental Medicine of the Czech Academy of Sciences Videnska 1083 142 20 Prague Czech Republic

Institute of Mathematics Faculty of Mechanical Engineering Brno University of Technology Technicka 2 616 69 Brno Czech Republic

University Centre for Energy Efficient Buildings Czech Technical University Prague Trinecka 1024 273 43 Bustehrad Czech Republic

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Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

Nanomaterials in Skin Regeneration and Rejuvenation