Analysis of hyaluronan and its derivatives using chromatographic and mass spectrometric techniques
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Review
PubMed
33049874
DOI
10.1016/j.carbpol.2020.117014
PII: S0144-8617(20)31187-5
Knihovny.cz E-resources
- Keywords
- Glycosaminoglycans, Hyaluronic acid, Liquid chromatography analysis, Mass spectrometric analysis, Oligosaccharides, Polysaccharide derivative,
- MeSH
- Amination MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Hyaluronic Acid analysis chemistry MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Names of Substances
- Hyaluronic Acid MeSH
The aim of this paper is to review chromatographic and mass-spectrometric methods and underline the best analytical approaches for successful analysis of various hyaluronic acid species in different types of samples. Hyaluronan-degrading enzymes and chemical depolymerization produce di- or oligosaccharides suitable for hyaluronan quantification or structural characterization of hyaluronan derivatives. Efficient purification and pre-column derivatization of hyaluronan disaccharides by reductive amination allow subnanogram quantification in biological samples. The chromatographic separation is capable to distinguish all glycosaminoglycans disaccharides and to resolve hyaluronan fragments with 2-40 monomers. Using electrospray ionization or matrix assisted laser desorption ionization, hyaluronan fragments up to 8 kDa or 41 kDa, respectively, can be observed. One- or two-dimensional chromatographic separation with higly sensitive mass-spectrometric detection is an indispensable tool for revealing substituent position, extent of modification and substitution patterns of chemically modified hyaluronan derivatives. It is essential for studying structure-biological function relationships of hyaluronan and its derivatives.
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