Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
33278999
DOI
10.1016/j.carbpol.2020.117244
PII: S0144-8617(20)31417-X
Knihovny.cz E-resources
- Keywords
- Conducting composites, Human induced pluripotent stem cells, Polyaniline, Polysacharides,
- MeSH
- Aniline Compounds chemistry MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- Biocompatible Materials chemistry pharmacology MeSH
- Cell Adhesion drug effects MeSH
- Cell Line MeSH
- Chitosan chemistry MeSH
- Electric Conductivity MeSH
- Escherichia coli drug effects MeSH
- Induced Pluripotent Stem Cells drug effects metabolism MeSH
- Hyaluronic Acid chemistry MeSH
- Humans MeSH
- Nanocomposites chemistry MeSH
- Polymerization MeSH
- Surface Properties MeSH
- Cell Proliferation drug effects MeSH
- Staphylococcus aureus drug effects MeSH
- Tissue Engineering methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- aniline MeSH Browser
- Aniline Compounds MeSH
- Anti-Bacterial Agents MeSH
- Biocompatible Materials MeSH
- Chitosan MeSH
- Hyaluronic Acid MeSH
- polyaniline MeSH Browser
Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH). The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC). Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films. The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues. The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2. Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH). The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.
Masaryk University Faculty of Science 625 00 Brno Czech Republic
University of Chemistry and Technology Prague Central Laboratories 166 28 Prague 6 Czech Republic
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