Telomere length in peripheral blood lymphocytes related to genetic variation in telomerase, prognosis and clinicopathological features in breast cancer patients
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
33367858
DOI
10.1093/mutage/geaa030
PII: 6050628
Knihovny.cz E-resources
- MeSH
- Alleles MeSH
- Genome-Wide Association Study MeSH
- Adult MeSH
- Genetic Predisposition to Disease genetics MeSH
- Genetic Variation genetics MeSH
- Genotype MeSH
- Telomere Homeostasis genetics MeSH
- Polymorphism, Single Nucleotide genetics MeSH
- Leukocytes, Mononuclear MeSH
- Leukocytes pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymphatic Metastasis genetics pathology MeSH
- Biomarkers, Tumor genetics MeSH
- Breast Neoplasms blood genetics pathology MeSH
- RNA genetics MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Neoplasm Staging MeSH
- Telomerase genetics MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers, Tumor MeSH
- RNA MeSH
- Telomerase MeSH
- telomerase RNA MeSH Browser
- TERT protein, human MeSH Browser
Disruption of telomere length (TL) homeostasis in peripheral blood lymphocytes has been previously assessed as a potential biomarker of breast cancer (BC) risk. The present study addressed the relationship between lymphocyte TL (LTL), prognosis and clinicopathological features in the BC patients since these associations are insufficiently explored at present. LTL was measured in 611 BC patients and 154 healthy controls using the monochrome multiplex quantitative Polymerase Chain Reaction assay. In addition, we genotyped nine TL-associated single-nucleotide polymorphisms that had been identified through genome-wide association studies. Our results showed that the patients had significantly (P = 0.001, Mann-Whitney U-test) longer LTL [median (interquartile range); 1.48 (1.22-1.78)] than the healthy controls [1.27 (0.97-1.82)]. Patients homozygous (CC) for the common allele of hTERT rs2736108 or the variant allele (CC) of hTERC rs16847897 had longer LTL. The latter association remained statistically significant in the recessive genetic model after the Bonferroni correction (P = 0.004, Wilcoxon two-sample test). We observed no association between LTL and overall survival or relapse-free survival of the patients. LTL did not correlate with cancer staging based on Union for International Cancer Control (UICC), The tumor node metastasis (TNM) staging system classification, tumour grade or molecular BC subtypes. Overall, we observed an association between long LTL and BC disease and an association of the hTERC rs16847897 CC genotype with increased LTL. However, no association between LTL, clinicopathological features and survival of the patients was found.
Division of Cancer Epidemiology German Cancer Research Center Im Neuenheimer Feld Heidelberg Germany
References provided by Crossref.org
The dynamics of telomere length in primary and metastatic colorectal cancer lesions
