Three new species of Cytauxzoon in European wild felids
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
33465567
DOI
10.1016/j.vetpar.2021.109344
PII: S0304-4017(21)00004-2
Knihovny.cz E-zdroje
- Klíčová slova
- 18S rDNA, Cytauxzoon, European wild felids, Genotypes, Mitochondrial genes, Piroplasmids,
- MeSH
- divoká zvířata parazitologie MeSH
- druhová specificita MeSH
- Felidae parazitologie MeSH
- fylogeneze MeSH
- genom mitochondriální MeSH
- genom protozoální MeSH
- Piroplasmida klasifikace genetika izolace a purifikace MeSH
- protozoální DNA genetika MeSH
- protozoální infekce zvířat epidemiologie parazitologie MeSH
- RNA ribozomální 18S genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Evropa MeSH
- Názvy látek
- protozoální DNA MeSH
- RNA ribozomální 18S MeSH
Protists of the genus Cytauxzoon infect a wide variety of wild and domestic felids worldwide. While the American Cytauxzoon felis has been well described, data on the European isolates of Cytauxzoon are still scant. The aim of the current study was to determine the genetic diversity of European Cytauxzoon spp. in wild felids across Europe by analyzing one nuclear and two mitochondrial genes, along with representative complete mitochondrial genomes. Overall, 106 biological samples from wild felids (92 from Felis silvestris and 14 from Lynx lynx) from Germany, Romania, Czech Republic, and Luxembourg were collected and screened for the presence of Cytauxzoon spp. using nested PCR protocols, targeting the highly conserved 18S rDNA, mitochondrial cytochrome b (CytB) and cytochrome c oxidase subunit I (COI) genes. Furthermore, 18 previously confirmed wild felid biological samples from Europe, and comparative material from USA positive for C. felis, were included in the study. In 18S rDNA sequences analyses, Cytauxzoon spp. from felids formed two separate clades of New World and Old World isolates, with a low inner diversity of the European clade. In contrast to 18S rDNA, the phylogenetic analyses of CytB and COI genes affirmatively revealed three highly supported clades, resulting in three defined genotypes. Similar intra- and interspecific variability of CytB and COI genes was observed in the case of different Babesia spp. Considering geography, host species and analyses of three genes, we conclude that the three detected genotypes of Cytauxzoon in European wild felids represent three new species, which we herein describe.
Department of Biological Sciences Eastern Illinois University 61920 3099 Charleston IL United States
Department of Veterinary Medicine University of Perugia 06126 Perugia Italy
Museum of Natural History 25 Rue Munster 2160 Luxembourg City Luxembourg
Citace poskytuje Crossref.org
Babesia pisicii n. sp. and Babesia canis Infect European Wild Cats, Felis silvestris, in Romania
