The objective of this study was to determine and evaluate the impact of the age and housing system on blood indicators (triacylglycerides, total cholesterol, aspartate aminotransferase, total proteins, albumin, glucose) and physical egg quality parameters (egg weight, shape index and surface area, eggshell proportion, thickness, strength, and color, albumen proportion and index, Haugh units, yolk proportion, index and yolk-to-albumen ratio) in selected native breeds of the Czech Republic (the Czech Golden Spotted hens) and Slovakia (the Oravka hens). Furthermore, the concentration of cholesterol in the yolk was determined. A total of 132 animals were used. There were 60 eggs collected from each breed at each monitored period for the evaluation of egg quality. Blood samples were taken by puncture of a wing vein. The assessments were made when the hens were of 34, 42, and 50 weeks old. Enriched cages and floor pens with litter were used as housing systems. The effects of breed, housing system, and age were observed. Furthermore, interactions among these factors were calculated. The significant effect of housing system was found in total cholesterol (P = 0.098) and aspartate aminotransferase (P = 0.0343) and the significant effect of age in total protein (P = 0.0392). The significant effect of breed (P = 0.0199), housing system (P = 0.0001), and age (P = 0.0001) was found in concentration of cholesterol in the yolk. Regarding the egg quality, the significant effect of breed (P = 0.0001) was found in eggshell color, albumen index and Haugh units, whereas the significant effect of housing system was found in egg weight (P = 0.0002), egg surface area (P = 0.0003), eggshell proportion (P = 0.0460), thickness (P = 0.0216), strength (P = 0.0049), and color (P = 0.0009). The significant effect of age was determined in all parameters except for the eggshell proportion and strength. The results represent an interesting comparison of changes in biochemical blood and egg quality parameters. It is necessary to further evaluate these indicators, especially in other genetic resources of hens, where the data are often nonexisting.

Czech University of Life Sciences Prague Faculty of Agrobiology Food and Natural Resources Department of Animal Science 165 00 Prague Suchdol Czech Republic

Czech University of Life Sciences Prague Faculty of Agrobiology Food and Natural Resources Department of Veterinary Sciences 165 00 Prague Suchdol Czech Republic

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Anderle V., Lichovníková M., Przywarová A., Dračková E. Egg quality of gene reserve the Czech golden spotted hens. Acta Fytotechn. Zootechn. 2014;17:84–86.

Basmacioğlu H., Ergül M. Research on the factors affecting cholesterol content and some other characteristics of eggs in laying hens. The effects of genotype and rearing system. Turk. J. Vet. Anim. Sci. 2005;29:157–164.

Begli H.E., Zerehdaran S., Hassani S., Abbasi M.A., Ahmadi A.K. Heritability, genetic and phenotypic correlations of egg quality traits in Iranian native fowl. Br. Poult. Sci. 2010;51:740–744. PubMed

Belew A.K., Tesfaye K., Belay G., Assefa G. The state of conservation of animal genetic resources in developing countries: a review. Int. J. Pharm. 2016;5:58–66.

Burnham M.R., Peebles E.D., Branton S.L., Jones M.S., Gerard P.D. Effects of F-strain Mycoplasma gallisepticum inoculation at twelve weeks of age on the blood characteristics of commercial egg laying hens. Poult. Sci. 2003;82:1397–1402. PubMed

Cerolini S., Baldi A., Cavalchini L.G. Blood and plasma biochemical variables in laying hens of different strains and ages. Arch. Geflügelkd. 1990;54:190–194.

Cheng H.W., Dillworth G., Singleton P., Chen Y., Muirt W.M. Effects of group selection for productivity and longevity on blood concentrations of serotonin, catecholamines, and corticosterone of laying hens. Poult. Sci. 2001;80:1278–1285. PubMed

Everds N.E., Snyder P.W., Bailey K.L., Bolon B., Creasy D.M., Foley G.L., Rosol T.J., Sellers T. Interpreting stress responses during routine toxicity studies: a review of the biology, impact, and assessment. Toxicol. Pathol. 2013;41:560–614. PubMed

Gallenberger M., Castell W., Hense B.A., Kuttler C. Dynamics of glucose and insulin concentration connected to the β-cell cycle: model development and analysis. Theor. Biol. Med. Model. 2012;9:46. PubMed PMC

Galli G.M., Da Silva A.S., Biazus A.H., Reis J.H., Boiago M.M., Topazio J.P., Migliorini M.J., Guarda N.S., Moresco R.N., Ourique A.F., Santos C.G., Lopes L.S., Baldissera M.D., Stefani L.M. Feed addition of curcumin to laying hens showed anticoccidial effect, and improved egg quality and animal health. Res. Vet. Sci. 2018;118:101–106. PubMed

Goncalves F.M., Ribeiro E.D., Montagner P., Lopes M.S., Anciuti M.A., Gentilini F.P., del Pino F.A.B., Correa M.N. Serum levels of liver enzymes in laying hens before and during the peak of egg production. Acta Sci. Vet. 2010;38:311–314.

Greene S.J., Gheorghiade M., Vaduganathan M., Ambrosy A.P., Mentz R.J., Subacius H., Maggioni A.P., Nodari S., Konstam M.A., Butler J., Filippatos G. Haemoconcentration, renal function, and post-discharge outcomes among patients hospitalized for heart failure with reduced ejection fraction: insights from the EVEREST trial. Eur. J. Heart Fail. 2013;15:1401–1411. PubMed PMC

Griffin H.D. Manipulation of egg yolk cholesterol: a physiologist's view. Worlds Poult. Sci. J. 1992;48:101–112.

Gyenis J., Sütő Z., Romvári R., Horn P. Tracking the development of serum biochemical parameters in two laying hen strains – a comparative study. Arch. Tierz. 2006;49:593–606.

Hanusová E., Hrnčár C., Hanus A., Oravcová M. Effect of breed on some traits of egg quality in laying hens. Acta Fytotechn. Zootechn. 2015;18:20–24.

Hanusová E., Oravcová M., Hanus A., Hrnčár C. Factors affecting growth in native Oravka chicken breed. Slovak J. Anim. 2017;50:112–117.

Hernandez J.M., Beardsworth P., Weber G. Egg quality – meeting consumer expectations. Int. Poult. Prod. 2005;13:20–23.

Ketta M., Tůmová E. Differences in the eggshell quality and tibia strength in Lohmann White and Czech Hen housed in cages and on litter. Acta Fytotechn. Zootechn. 2014;17:75–78.

Koronowicz A.A., Banks P., Szymczyk B., Leszczyńska T., Master A., Piasna E., Szczepański W., Domagała D., Kopeć A., Piątkowska E., Laidler P. Dietary conjugated linoleic acid affects blood parameters, liver morphology and expression of selected hepatic genes in laying hens. Br. Poult. Sci. 2016;57:663–673. PubMed

Kraus A., Zita L., Krunt O. The effect of different housing system on quality parameters of eggs in relationship to the age in brown egg-laying hens. Bulg. J. Agric. Sci. 2019;25:1246–1253.

Krawczyk J., Sokołowicz Z., Szymczy B. Effect of housing system on cholesterol, vitamin and fatty acid content of yolk and physical characteristics of eggs from Polish native hens. Arch. Geflügelkd. 2011;75:151–157.

Ledvinka Z., Tůmová E., Englmaierová M., Podsedníček M. Egg quality of three laying hen genotypes kept in conventional cages and on litter. Arch. Geflügelk. 2012;76:38–43.

Lewko L., Gornowicz E. Effect of housing system on egg quality in laying hens. Ann. Anim. Sci. 2011;11:607–616.

Marono S., Loponte R., Lombardi P., Vassalotti G., Pero M.E., Russo F., Gasco L., Parisi G., Piccolo G., Nizza S., Di Meo C., Attia Y.A., Bovera F. Productive performance and blood profiles of laying hens fed Hermetia illucens larvae meal as total replacement of soybean meal from 24 to 45 weeks of age. Poult. Sci. 2017;96:1783–1790. PubMed

Matt D., Veromann E., Luik A. Effect of housing systems on biochemical composition of chicken eggs. Agron. Res. 2009;7:662–667.

Mollahosseini M., Daneshzad E., Rahimi M.H., Yekaninejad M.S., Maghbooli Z., Mirzaei K. The association between fruit and vegetable intake and liver enzymes (aspartate and alanine transaminases) in Tehran, Iran. Ethiop. J. Health Sci. 2017;27:401–410. PubMed PMC

Narushin V.G., Romanov M.N., Griffin D.K. A novel egg quality index as an alternative to Haugh unit score. J. Food Eng. 2020;289:110176.

Nasrel-din R.A., Yousef A.A., Mahmoud M.F., Ibrahim I.A. Thyroid-hormones, glucose, total lipids and total proteins in blood-serum before and during laying in hisex-brown chickens. J. Anim. Physiol. Anim. Nutr. 1988;59:167–170.

Onbasilar E.E., Aksoy F.T. Stress parameters and immune response of layers under different cage floor and density condition. Livest. Prod. Sci. 2005;95:255–263.

Pavlík A., Pokludová D., Zapletal P., Jelínek P. Effects of housing systems on biochemical indicators of blood plasma in laying hens. Acta Vet. Brno. 2007;76:339–347.

Pillutla P., Hwang Y.C., Augustus A., Yokoyama M., Yagyu H., Johnston T.P., Kaneko M., Ramasamy R., Goldberg I.J. Perfusion of hearts with triglyceride-rich particles reproduces the metabolic abnormalities in lipotoxic cardiomyopathy. Am. J. Physiol-endoc. M. 2005;288:1229–1235. PubMed

Puvadolpirod S., Thaxton J. Model of physiological stress in chickens 1. Response parameters. Poult. Sci. 2000;79:370–376. PubMed

Rahmani D., Kallas Z., Pappa M., Gil J.M. Are consumers’ egg Preferences influenced by animal-welfare conditions and environmental impacts? Sustainability. 2019;11:23.

Rizzi C., Chiericato G.M. Chemical composition of meat and egg yolk of hybrid and Italian breed hens reared using an organic production system. Poult. Sci. 2010;89:1239–1251. PubMed

Samiullah S., Omar A.S., Roberts J., Chousalkar K. Effect of production system and flock age on eggshell and egg internal quality measurements. Poult. Sci. 2017;96:246–258. PubMed

Samiullah S., Roberts J.R., Chousalkar K. Eggshell colour in brown-egg laying hens – a review. Poult. Sci. 2015;94:2566–2575. PubMed PMC

Sapkota S., Kolachhapati M.R., Devkota N.R., Gorkhali N.A., Bhattarai N. Evaluation of egg laying and egg quality parameters of local chicken Sakini (Gallus gallus domesticus) of Nepal. J. Agri. For. Uni. 2017;1:181–188.

Shivaprasad H.L., Jaap R.G. Egg and yolk production as influenced by liver weight, liver lipid and plasma lipid in three strains of small bodied chickens. Poult. Sci. 1977;56:1384–1390.

Simaraks S., Chinrasri O., Aengwanich W. Haematological, electrolyte and serum biochemical values of the Thai indigenous chickens (Gallus domesticus) in northern Thailand. Songhlanakarin J. Sci. Technol. 2004;26:425–430.

Sirri F., Zampiga M., Berardinelli A., Meluzzi A.A. Variability and interaction of some egg physical and eggshell quality attributes during the entire laying hen cycle. Poult. Sci. 2018;97:1818–1823. PubMed

Sokołowicz Z., Dykiel M., Krawczyk J., Augustyńska-Prejsnar A. Effect of layer genotype on physical characteristics and nutritive value of organic eggs. Cyta-j. Food. 2019;17:11–19.

Sokołowicz Z., Krawczyk J., Dykiel M. Effect of alternative housing system and hen genotype on egg quality characteristics. Emir. J. Food Agric. 2018;30:695–703.

Suchý P., Straková E., Hrubý A. Variation in cholesterol concentrations in the blood plasma of hens throughout the laying period. Czech J. Anim. Sci. 1999;44:109–111.

Suchý P., Straková E., Večerek V., Šterc P. Biochemical studies of blood in hens during the laying period. Czech J. Anim. Sci. 2001;46:383–387.

Svobodová J., Tůmová E., Englmaierová M. The effect of housing system on egg quality of Lohmann white and Czech hen. Acta Fytotechn. Zootechn. 2014;17:44–46.

Tolimir N., Maslovarić M., Škrbić Z., Lukić M., Rajković B., Radišić R. Consumer criteria for purchasing eggs and the quality of eggs in the markets of the city of Belgrade. Biotechnol. Anim. Husb. 2017;33:425–437.

van den Brand H., Parmentier H.K., Kemp A.B. Effects of housing system (outdoor vs cages) and age of laying hens on egg characteristics. Br. Poult. Sci. 2004;45:745–752. PubMed

Vlčková J., Tůmová E., Ketta M., Englmaierová M., Chodová D. Effect of housing system and age of laying hens on eggshell quality, microbial contamination, and penetration of microorganisms into eggs. Czech J. Anim. Sci. 2018;63:51–60.

Vogt H., Harnish S., Krieg R. Studies about the necessary level of the nicotinamide supplementation to broiler rations. Arch. Geflügelkd. 1990;54:45–49.

Yilmaz Dikmen B., Ipek A., Şahan Ü., Sözcü A., Baycan S.C. Impact of different housing systems and age of layers on egg quality characteristics. Turk. J. Vet. Anim. Sci. 2017;41:77–84.

Zaheer K. An Updated review on chicken eggs: production, Consumption, Management Aspects and nutritional Benefits to human health. Food Nutr. Sci. 2015;6:1208–1220.

Zemková Ľ., Simeonovová J., Lichovníková M., Somerlíková K. The effects of housing systems and age of hens on the weight and cholesterol concentration of the egg. Czech J. Anim. Sci. 2007;52:110–115.

Zhang J., Li Q., Wu Y., Wang D., Xu L., Zhang Y., Wang S., Wang T., Liu F., Zaky M.Y., Hou S., Liu S., Zou K., Lei H., Zou L., Zhang Y., Liu H. Cholesterol content in cell membrane maintains surface levels of ErbB2 and confers a therapeutic vulnerability in ErbB2-positive breast cancer. J. Cell Commun. Signal. 2019;17:15. PubMed PMC

Zita L., Tůmová E., Štolc L. Effects of genotype, age and their interaction on egg quality in brown-egg laying hens. Acta Vet. Brno. 2009;78:85–91.

Comparison of Commercial Poultry Semen Extenders Modified for Cryopreservation Procedure in the Genetic Resource Program of Czech Golden Spotted Hen

Changes in Fatty Acids Profile, Health Indices, and Physical Characteristics of Organic Eggs from Laying Hens at the Beginning of the First and Second Laying Cycles