This study was undertaken to investigate the starter and probiotic potential of lactic acid bacteria isolated from dromedarian camel's milk using both culture-dependent and -independent approaches and metataxonomic analysis. Strains of lactic acid bacteria recovered were examined in vitro for tolerance to gastric acidity, bile, and lysozyme. Bile salt hydrolysis, serum cholesterol-lowering, oxalate degradation, proteolytic activity, exopolysaccharide production, and cell surface characteristics necessary for colonizing intestinal mucosa were also evaluated. A single strain of the species, Lactobacillus fermentum named NPL280, was selected through multivariate analysis as it harbored potential probiotic advantages and fulfilled safety criteria. The strain assimilated cholesterol, degraded oxalate, produced exopolysaccharides, and proved to be a proficient alternate yogurt starter with good viability in stored bio-yogurt. A sensorial analysis of the prepared bio-yogurt was also found to be exemplary. We conclude that the indigenous L. fermentum strain NPL280 has the desired traits of a starter and adjunct probiotic culture for dairy products.

National Probiotic Lab NIBGE Jhang Road Faisalabad 38000 Punjab Pakistan

Pakistan Institute of Engineering and Applied Sciences Nilore 45650 Islamabad Pakistan

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Abdulla AA, Abed TA, Saeed A (2014) Adhesion, autoaggregation and hydrophobicity of six Lactobacillus strains. Br Microbiol Res J 4:381–391. https://doi.org/10.9734/BMRJ/2014/6462 DOI

Abouloifa H et al (2019) Characterization of probiotic properties of antifungal Lactobacillus strains isolated from traditional fermenting green olives. Probiotics Antimicrob Proteins 12:683–696. https://doi.org/10.1007/s12602-019-09543-8 DOI

Abushelaibi A, Al-Mahadin S, El-Tarabily K, Shah NP, Ayyash M (2017) Characterization of potential probiotic lactic acid bacteria isolated from camel milk. LWT Food Sci Technol 79:316–325. https://doi.org/10.1016/j.lwt.2017.01.041 DOI

Akabanda F, Owusu-Kwarteng J, Tano-Debrah K, Parkouda C, Jespersen L (2014) The use of lactic acid bacteria starter culture in the production of Nunu, a spontaneously fermented milk product in Ghana. Intl J Food Sci 2014:11. https://doi.org/10.1155/2014/721067 DOI

Alaoui Ismaili M, Saidi B, Zahar M, Hamama A, Ezzaier R (2019) Composition and microbial quality of raw camel milk produced in Morocco. J Saudi Soc Agric Sci 18:17–21. https://doi.org/10.1016/j.jssas.2016.12.001 DOI

Amrouche T, Mounier J, Pawtowski A, Thomas F, Picot A (2020) Microbiota associated with dromedary camel milk from Algerian Sahara. Curr Microbiol 77:24–31. https://doi.org/10.1007/s00284-019-01788-4 PubMed DOI

Anandharaj M, Sivasankari B, Santhanakaruppu R, Manimaran M, Rani RP, Sivakumar S (2015) Determining the probiotic potential of cholesterol-reducing Lactobacillus and Weissella strains isolated from gherkins (fermented cucumber) and south Indian fermented koozh. Res Microbiol 166:428–439. https://doi.org/10.1016/j.resmic.2015.03.002 PubMed DOI

Angmo K, Kumari A, Bhalla TC (2016) Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT - Food Sci Technol 66:428–435. https://doi.org/10.1016/j.lwt.2015.10.057 DOI

Anisimova E, Yarullina D (2018) Characterization of erythromycin and tetracycline resistance in Lactobacillus fermentum strains. Int J Med Microbiol 2018:9. https://doi.org/10.1155/2018/3912326 DOI

Arena M et al (2015) Functional starters for functional yogurt Foods 4:15–33. https://doi.org/10.3390/foods4010015 PubMed DOI

Arslan S, Bayrakci S (2016) Physicochemical, functional, and sensory properties of yogurts containing persimmon. Turk J Agric For 40:68–74. https://doi.org/10.3906/tar-1406-150 DOI

Asan-Ozusaglam M, Gunyakti A (2019) Lactobacillus fermentum strains from human breast milk with probiotic properties and cholesterol-lowering effects. Food Sci Biotechnol 28:501–509. https://doi.org/10.1007/s10068-018-0494-y PubMed DOI

Ayyash M, Al-Nuaimi AK, Al-Mahadin S, Liu SQ (2018) In vitro investigation of anticancer and ACE-inhibiting activity, alpha-amylase and alpha-glucosidase inhibition, and antioxidant activity of camel milk fermented with camel milk probiotic: a comparative study with fermented bovine milk. Food Chem 239:588–597. https://doi.org/10.1016/j.foodchem.2017.06.149 PubMed DOI

Barbieri F, Montanari C, Gardini F, Tabanelli G (2019) Biogenic amine production by lactic acid bacteria: a review. Foods 8:17. https://doi.org/10.3390/foods8010017 DOI PMC

Batista A et al (2017) Developing a synbiotic fermented milk using probiotic bacteria and organic green banana flour. J Funct Foods 38:242–250. https://doi.org/10.1016/j.jff.2017.09.037 DOI

Belicová A, Mikulášová M, Dušinský R (2013) Probiotic potential and safety properties of Lactobacillus plantarum from Slovak Bryndza cheese. Biomed Res Int 2013:8. https://doi.org/10.1155/2013/760298 DOI

Benkerroum N (2016) Biogenic amines in dairy products: origin, incidence, and control means. Compr Rev Food Sci Food Saf 15:801–826. https://doi.org/10.1111/1541-4337.12212 PubMed DOI

Benmechernene Z, Chentouf HF, Yahia B, Fatima G, Quintela-Baluja M, Calo-Mata P, Barros-Velázquez J (2013) Technological aptitude and applications of Leuconostoc mesenteroides bioactive strains isolated from Algerian raw camel milk. Biomed Res Int 2013:14. https://doi.org/10.1155/2013/418132 DOI

Bubnov RV, Babenko LP, Lazarenko LM, Mokrozub VV, Spivak MY (2018) Specific properties of probiotic strains: relevance and benefits for the host. EPMA J 9:205–223. https://doi.org/10.1007/s13167-018-0132-z PubMed DOI PMC

Câmara S, Dapkevicius A, Riquelme C, Elias R, Silva C, Malcata F, Dapkevicius M (2019) Potential of lactic acid bacteria from Pico cheese for starter culture development. Food Sci Technol Int 25:303–317. https://doi.org/10.1177/1082013218823129 PubMed DOI

Campedelli I et al (2019) Genus-wide assessment of antibiotic resistance in Lactobacillus spp. Appl Env Microbiol 85:e01738-e11718. https://doi.org/10.1128/AEM.01738-18 DOI

Caporaso JG et al (2011) Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci USA 108:4516–4522. https://doi.org/10.1073/pnas.1000080107 PubMed DOI

Caro I, Mateo J, Sandoval MH, Soto S, García-Armesto MR, Castro JM (2013) Characterization of Oaxaca raw milk cheese microbiota with particular interest in Lactobacillus strains. J Dairy Sci 96:3461–3470. https://doi.org/10.3168/jds.2012-6103 PubMed DOI

Casarotti SN, Carneiro BM, Todorov SD, Nero LA, Rahal P, Penna ALB (2017) In vitro assessment of safety and probiotic potential characteristics of Lactobacillus strains isolated from water buffalo mozzarella cheese. Ann Microbiol 67:289–301. https://doi.org/10.1007/s13213-017-1258-2 DOI

Chandan RC, Gandhi A, Shah NP (2017) Yogurt: historical background, health benefits, and global trade. In: Shah NP (ed) Yogurt in Health and Disease Prevention. Academic Press, pp 3–29. https://doi.org/10.1016/B978-0-12-805134-4.00001-8

Chatterjee M, Pushkaran AC, Vasudevan AK, Menon KKN, Biswas R, Mohan CG (2018) Understanding the adhesion mechanism of a mucin binding domain from Lactobacillus fermentum and its role in enteropathogen exclusion. Int J Biol Macromol 110:598–607. https://doi.org/10.1016/j.ijbiomac.2017.10.107 PubMed DOI

Chen KH, Ki M, Sugihara H, Araki Y, Yamamoto G, Hattori T (2007) High animal-fat intake changes the bile-acid composition of bile juice and enhances the development of Barrett’s esophagus and esophageal adenocarcinoma in a rat duodenal-contents reflux model. Cancer Sci 98:1683–1688. https://doi.org/10.1111/j.1349-7006.2007.00605.x PubMed DOI

Chervinets Y, Chervinets V, Shenderov B, Belyaeva E, Troshin A, Lebedev S, Danilenko V (2018) Adaptation and probiotic potential of lactobacilli, isolated from the oral cavity and intestines of healthy people. Probiotics Antimicrob Proteins 10:22–33. https://doi.org/10.1007/s12602-017-9348-9 PubMed DOI

Cho WY, Hong GE, Lee HJ, Yeon SJ, Paik HD, Hosaka YZ, Lee CH (2020) Effect of yogurt fermented by Lactobacillus Fermentum TSI and L. Fermentum S2 derived from a Mongolian traditional dairy product on rats with high-fat-diet-induced obesity. Foods 9:12.  https://doi.org/10.3390/foods9050594

Cliff M, Fan L, Sanford K, Stanich K, Doucette C, Raymond N (2013) Descriptive analysis and early-stage consumer acceptance of yogurts fermented with carrot juice. J Dairy Sci 96:4160–4172. https://doi.org/10.3168/jds.2012-6287 PubMed DOI

Cornick S, Tawiah A, Chadee K (2015) Roles and regulation of the mucus barrier in the gut. Tissue barriers 3:e982426. https://doi.org/10.4161/21688370.2014.982426 PubMed DOI PMC

Dąbrowska A, Babij K, Szołtysik M, Chrzanowska J (2017) Viability and growth promotion of starter and probiotic bacteria in yogurt supplemented with whey protein hydrolysate during refrigerated storage. Adv Hygiene Exp Med 71:952–959. https://doi.org/10.5604/01.3001.0010.5866 DOI

Das K, Choudhary R, Thompson-Witrick KA (2019) Effects of new technology on the current manufacturing process of yogurt-to increase the overall marketability of yogurt. LWT Food Sci Technol 108:69–80. https://doi.org/10.1016/j.lwt.2019.03.058 DOI

de Melo Pereira GV, de Oliveira CB, Júnior AIM, Thomaz-Soccol V, Soccol CR (2018) How to select a probiotic? A review and update of methods and criteria. Biotechnol Adv 36:2060–2076. https://doi.org/10.1016/j.biotechadv.2018.09.003 PubMed DOI

de Moraes GMD et al (2017) Functional properties of Lactobacillus mucosae strains isolated from Brazilian goat milk. Probiotics Antimicrob Proteins 9:235–245. https://doi.org/10.1007/s12602-016-9244-8 PubMed DOI

de Souza BMS, Borgonovi TF, Casarotti SN, Todorov SD, Penna ALB (2019) Lactobacillus casei and Lactobacillus fermentum strains isolated from mozzarella cheese: probiotic potential, safety, acidifying kinetic parameters and viability under gastrointestinal tract conditions. Probiotics Antimicrob Proteins 11:382–396. https://doi.org/10.1007/s12602-018-9406-y PubMed DOI

Delgado S, Leite AM, Ruas-Madiedo P, Mayo B (2015) Probiotic and technological properties of Lactobacillus spp. strains from the human stomach in the search for potential candidates against gastric microbial dysbiosis. Front Microbiol 5:8. https://doi.org/10.3389/fmicb.2014.00766

Dinu V et al (2020) The antibiotic vancomycin induces complexation and aggregation of gastrointestinal and submaxillary mucins. Sci Rep 10:12. https://doi.org/10.1038/s41598-020-57776-3 DOI

dos Santos KMO et al (2015) Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains. Dairy Sci Technol 95:209–230. https://doi.org/10.1007/s13594-014-0201-6 DOI

Elbanna K, El Hadad S, Assaeedi A, Aldahlawi A, Khider M, Alhebshi A (2018) In vitro and in vivo evidences for innate immune stimulators lactic acid bacterial starters isolated from fermented camel dairy products. Sci Rep 8:12553. https://doi.org/10.1038/s41598-018-31006-3 PubMed DOI PMC

Endo A et al (2018) In vitro and in silico characterisation of Lactobacillus paraplantarum D2–1, a starter culture for soymilk fermentation. Int J Food Sci Nutr 69:857–869. https://doi.org/10.1080/09637486.2017.1422701 PubMed DOI

FAO-WHO, (2006) Probiotics in food: health and nutritional properties and guidelines for evaluation, vol 85. UN, Rome, Italy

FAO-WHO (2011) Milk & milk products. In: Codex Alimentarius. 2nd edn. United Nations, Rome, p 248

Faraz A, Mustafa MI, Lateef M, Yaqoob M, Younas M (2013) Production potential of camel and its prospects in Pakistan. Punjab Univ J Zool 28:89–95. 0079–8045/13/0089–0095

FEEDAP (2012) Guidance on studies concerning the safety of use of the additive for users/workers. EFSA J 10:1–5. https://doi.org/10.2903/j.efsa.2012.2539 DOI

Ferreira AB et al (2013) Increased expression of clp genes in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress and bile salts. Benef Microbes 4:367–374. https://doi.org/10.3920/BM2013.0022 PubMed DOI

Fguiri I, Ziadi M, Rekaya K, Samira A, Khorchani T (2017) Isolation and characterization of lactic acid bacteria strains from raw camel milk for potential use in the production of yogurt. J Food Sci Nutr 3:8. https://doi.org/10.24966/FSN-1076/100026

Folkenberg DM, Dejmek P, Skriver A, Ipsen R (2005) Relation between sensory texture properties and exopolysaccharide distribution in set and in stirred yoghurts produced with different starter cultures. J Texture Stud 36:174–189. https://doi.org/10.1111/j.1745-4603.2005.00010.x DOI

Ganda EK et al (2016) Longitudinal metagenomic profiling of bovine milk to assess the impact of intramammary treatment using a third-generation cephalosporin. Sci Rep 6:13. https://doi.org/10.1038/srep37565 DOI

Gayer CP, Basson MD (2009) The effects of mechanical forces on intestinal physiology and pathology. Cell Signal 21:1237–1244. https://doi.org/10.1016/j.cellsig.2009.02.011 PubMed DOI PMC

Georgieva R et al (2015) Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Equip 29:84–91. https://doi.org/10.1080/13102818.2014.987450 DOI

Gomathi S, Sasikumar P, Anbazhagan K, Sasikumar S, Kavitha M, Selvi M, Selvam GS (2014) Screening of indigenous oxalate degrading lactic acid bacteria from human faeces and South Indian fermented foods: assessment of probiotic potential. Sci World J 2014:11. https://doi.org/10.1155/2014/648059 DOI

Gomes A et al (2011) Effect of the inoculation level of Lactobacillus acidophilus in probiotic cheese on the physicochemical features and sensory performance compared with commercial cheeses. J Dairy Sci 94:4777–4786. https://doi.org/10.3168/jds.2011-4175 PubMed DOI

Granato D, Branco GF, Cruz AG, Faria JdAF, Shah NP (2010) Probiotic dairy products as functional foods. Compr Rev Food Sci Food Saf 9:455–470. https://doi.org/10.1111/j.1541-4337.2010.00120.x PubMed DOI

Gueimonde M, Sánchez B, de los Reyes-Gavilán CG, Margolles A, (2013) Antibiotic resistance in probiotic bacteria. Front Microbiol 4:202. https://doi.org/10.3389/fmicb.2013.00202 PubMed DOI PMC

Guglielmotti D, Marcó MB, Vinderola C, de los Reyes Gavilán C, Reinheimer J, Quiberoni A, (2007) Spontaneous Lactobacillus delbrueckii phage-resistant mutants with acquired bile tolerance. Int J Food Microbiol 119:236–242. https://doi.org/10.1016/j.ijfoodmicro.2007.08.010 PubMed DOI

Hamed E, Elattar A (2013) Identification and some probiotic potential of lactic acid bacteria isolated from Egyptian camels milk. Life Sci J 10:1952–1961

Heo S, Lee JH, Jeong DW (2020) Food-derived coagulase-negative Staphylococcus as starter cultures for fermented foods. Food Sci Biotechnol 29:1023–1035. https://doi.org/10.1007/s10068-020-00789-5 PubMed DOI PMC

Ibrahem SA, El Zubeir IE (2016) Processing, composition and sensory characteristic of yoghurt made from camel milk and camel–sheep milk mixtures. Small Rumin Res 136:109–112. https://doi.org/10.1016/j.smallrumres.2016.01.014 DOI

Illeghems K, De Vuyst L, Weckx S (2015) Comparative genome analysis of the candidate functional starter culture strains Lactobacillus fermentum 222 and Lactobacillus plantarum 80 for controlled cocoa bean fermentation processes. BMC Genomics 16:13. https://doi.org/10.1186/s12864-015-1927-0 DOI

Jans C, Bugnard J, Njage PMK, Lacroix C, Meile L (2012) Lactic acid bacteria diversity of African raw and fermented camel milk products reveals a highly competitive, potentially health-threatening predominant microflora. LWT Food Sci Technol 47:371–379. https://doi.org/10.1016/j.lwt.2012.01.034 DOI

Jose N, Bunt C, Hussain M (2015) Comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents. Microorganisms 3:198–212. https://doi.org/10.3390/microorganisms3020198 PubMed DOI PMC

Kakde S, Bhopal RS, Bhardwaj S, Misra A (2017) Urbanized South Asians’ susceptibility to coronary heart disease: the high-heat food preparation hypothesis. Nutrition 33:216–224. https://doi.org/10.1016/j.nut.2016.07.006 PubMed DOI

Kotzamanidis C, Kourelis A, Litopoulou-Tzanetaki E, Tzanetakis N, Yiangou M (2010) Evaluation of adhesion capacity, cell surface traits and immunomodulatory activity of presumptive probiotic Lactobacillus strains. Int J Food Microbiol 140:154–163. https://doi.org/10.1016/j.ijfoodmicro.2010.04.004 PubMed DOI

Kumar D (2016) Camel milk: alternative milk for human consumption and its health benefits. Nutr Food Sci 46:217–227. https://doi.org/10.1108/NFS-07-2015-0085 DOI

Kumar M et al (2012) Cholesterol-lowering probiotics as potential biotherapeutics for metabolic diseases. J Diabetes Res 2012:15. https://doi.org/10.1155/2012/902917 DOI

Lee KK, Yii KC (1996) A comparison of three methods for assaying hydrophobicity of pathogenic vibrios. Lett Appl Microbiol 23:343–346. https://doi.org/10.1111/j.1472-765X.1996.tb00204.x DOI

Leite AM, Miguel M, Peixoto R, Ruas-Madiedo P, Paschoalin V, Mayo B, Delgado S (2015) Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains. J Dairy Sci 98:3622–3632. https://doi.org/10.3168/jds.2014-9265 PubMed DOI

Luckow T, Sheehan V, Fitzgerald G, Delahunty C (2006) Exposure, health information and flavour-masking strategies for improving the sensory quality of probiotic juice. Appetite 47:315–323. https://doi.org/10.1016/j.appet.2006.04.006 PubMed DOI

Mabood F et al (2017) Development of new NIR-spectroscopy method combined with multivariate analysis for detection of adulteration in camel milk with goat milk. Food Chem 221:746–750. https://doi.org/10.1016/j.foodchem.2016.11.109 PubMed DOI

Mani-López E, Palou E, López-Malo A (2014) Probiotic viability and storage stability of yogurts and fermented milks prepared with several mixtures of lactic acid bacteria. J Dairy Sci 97:2578–2590. https://doi.org/10.3168/jds.2013-7551 PubMed DOI

Masiello S, Martin N, Watters R, Galton D, Schukken Y, Wiedmann M, Boor K (2014) Identification of dairy farm management practices associated with the presence of psychrotolerant sporeformers in bulk tank milk. J Dairy Sci 97:4083–4096. https://doi.org/10.3168/jds.2014-7938 PubMed DOI

Meli F, Lazzi C, Neviani E, Gatti M (2014) Effect of protein hydrolysates on growth kinetics and aminopeptidase activities of Lactobacillus. Curr Microbiol 68:82–87. https://doi.org/10.1007/s00284-013-0445-z PubMed DOI

Miremadi F, Ayyash M, Sherkat F, Stojanovska L (2014) Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic Lactobacilli and Bifidobacteria. J Funct Foods 9:295–305. https://doi.org/10.1016/j.jff.2014.05.002 DOI

Moeller R, Horneck G, Rettberg P, Mollenkopf H-J, Stackebrandt E, Nicholson W (2006) A method for extracting RNA from dormant and germinating Bacillus subtilis strain 168 endospores. Curr Microbiol 53:227–231. https://doi.org/10.1007/s00284-006-0099-1 PubMed DOI

Mu Q, Tavella VJ, Luo XM (2018) Role of Lactobacillus reuteri in human health and diseases. Front Microbiol 9:17. https://doi.org/10.3389/fmicb.2018.00757 DOI

Murphy C et al (2009) Metabolic activity of probiotics—oxalate degradation. Vet Microbiol 136:100–107. https://doi.org/10.1016/j.vetmic.2008.10.005 PubMed DOI

Naghmouchi K, Belguesmia Y, Bendali F, Spano G, Seal BS, Drider D (2019) Lactobacillus fermentum: a bacterial species with potential for food preservation and biomedical applications. Crit Rev Food Sci Nutr 60:3387–3399. https://doi.org/10.1080/10408398.2019.1688250 PubMed DOI

Nagy P, Fábri ZN, Varga L, Reiczigel J, Juhász J (2017) Effect of genetic and nongenetic factors on chemical composition of individual milk samples from dromedary camels (Camelus dromedarius) under intensive management. J Dairy Sci 100:8680–8693. https://doi.org/10.3168/jds.2017-12814 PubMed DOI

Nielsen CLM, Hornbaek T, Rasmussen P, Poulsen L (2018) Lactobacillus fermentum bacteria reducing the concentration of acetaldehyde

Owusu-Kwarteng J, Tano-Debrah K, Akabanda F, Jespersen L (2015) Technological properties and probiotic potential of Lactobacillus fermentum strains isolated from West African fermented millet dough. BMC Microbiol 15:10. https://doi.org/10.1186/s12866-015-0602-6 DOI

Panicker AS, Ali SA, Anand S, Panjagari NR, Kumar S, Mohanty A, Behare PV (2018) Evaluation of some in vitro probiotic properties of Lactobacillus fermentum strains. J Food Sci Technol 55:2801–2807. https://doi.org/10.1007/s13197-018-3197-8 PubMed DOI PMC

Pessione E, Cirrincione S (2016) Bioactive molecules released in food by lactic acid bacteria: Encrypted peptides and biogenic amines. Front Microbiol 7:19. https://doi.org/10.3389/fmicb.2016.00876 DOI

Pisano MB, Viale S, Conti S, Fadda ME, Deplano M, Melis MP, Deiana M, Cosentino S (2014) Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian dairy products. BioMed Res Int 2014. https://doi.org/10.1155/2014/286390

Pradhan D, Mallappa RH, Grover S (2020) Comprehensive approaches for assessing the safety of probiotic bacteria. Food Control 108:106872. https://doi.org/10.1016/j.foodcont.2019.106872 DOI

Rajab S, Tabandeh F, Shahraky MK, Alahyaribeik S (2020) The effect of Lactobacillus cell size on its probiotic characteristics. Anaerobe 62:102103. https://doi.org/10.1016/j.anaerobe.2019.102103 PubMed DOI

Razmgah N, Mojgani N, Torshizi M (2016) Probiotic potential and virulence traits of Bacillus and Lactobacillus species isolated from local honey sample in Iran. IOSR J Pharm Biol Sci 11:87–95. https://doi.org/10.9790/3008-1105048795 DOI

Ren D et al (2014) In vitro evaluation of the probiotic and functional potential of Lactobacillus strains isolated from fermented food and human intestine. Anaerobe 30:1–10. https://doi.org/10.1016/j.anaerobe.2014.07.004 PubMed DOI

Ru X, Zhang CC, Yuan YH, Yue TL, Guo CF (2019) Bile salt hydrolase activity is present in nonintestinal lactic acid bacteria at an intermediate level. Appl Microbiol Biotechnol 103:893–902. https://doi.org/10.1007/s00253-018-9492-5 PubMed DOI

Rutella GS, Tagliazucchi D, Solieri L (2016) Survival and bioactivities of selected probiotic lactobacilli in yogurt fermentation and cold storage: new insights for developing a bi-functional dairy food. Food Microbiol 60:54–61. https://doi.org/10.1016/j.fm.2016.06.017 PubMed DOI

Sah B, Vasiljevic T, McKechnie S, Donkor O (2014) Effect of probiotics on antioxidant and antimutagenic activities of crude peptide extract from yogurt. Food Chem 156:264–270. https://doi.org/10.1016/j.foodchem.2014.01.105 PubMed DOI

Saikia D et al (2018) Hypocholesterolemic activity of indigenous probiotic isolate Saccharomyces cerevisiae ARDMC1 in a rat model. J Food Drug Anal 26:154–162. https://doi.org/10.1016/j.jfda.2016.12.017 PubMed DOI

Salas-Jara MJ, Ilabaca A, Vega M, García A (2016) Biofilm forming Lactobacillus: new challenges for the development of probiotics. Microorganisms 4. https://doi.org/10.3390/microorganisms4030035

Sanz T, Salvador A, Jimenez A, Fiszman S (2008) Yogurt enrichment with functional asparagus fibre. Effect of fibre extraction method on rheological properties, colour, and sensory acceptance. Eur Food Res Technol 227:1515–1521. https://doi.org/10.1007/s00217-008-0874-2 DOI

Senok AC, Ismaeel AY, Botta GA (2005) Probiotics: facts and myths. Clin Microbiol Inf 11:958–966. https://doi.org/10.1111/j.1469-0691.2005.01228.x DOI

Shah NP (2007) Functional cultures and health benefits. Int Dairy J 17:1262–1277. https://doi.org/10.1016/j.idairyj.2007.01.014 DOI

Shakerian M, Razavi SH, Ziai SA, Khodaiyan F, Yarmand MS, Moayedi A (2015) Proteolytic and ACE-inhibitory activities of probiotic yogurt containing non-viable bacteria as affected by different levels of fat, inulin and starter culture. J Food Sci Technol 52:2428–2433. https://doi.org/10.1007/s13197-013-1202-9 PubMed DOI

Sharma P, Tomar SK, Sangwan V, Goswami P, Singh R (2016) Antibiotic resistance of Lactobacillus sp. isolated from commercial probiotic preparations. J Food Saf 36:38–51. https://doi.org/10.1111/jfs.12211 DOI

Shokryazdan P, Jahromi M, Liang J, Sieo C, Kalavathy R, Idrus Z, Ho Y (2017) In vitro assessment of bioactivities of Lactobacillus strains as potential probiotics for humans and chickens. J Food Sci 82:2734–2745. https://doi.org/10.1111/1750-3841.13921 PubMed DOI

Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB, Faseleh Jahromi M, Ho YW (2014) Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. Biomed Res Int 2014:17. https://doi.org/10.1155/2014/927268 DOI

Shori AB (2017) Camel milk and its fermented products as a source of potential probiotic strains and novel food cultures: a mini review. PharmaNutrition 5:84–88. https://doi.org/10.1016/j.phanu.2017.06.003 DOI

Silva FA et al (2017) The effect of Isabel grape addition on the physicochemical, microbiological and sensory characteristics of probiotic goat milk yogurt. Food Funct 8:2121–2132. https://doi.org/10.1039/C6FO01795A PubMed DOI

Singh V, Arora V, Alam MJ, Garey KW (2012) Inhibition of biofilm formation by esomeprazole in Pseudomonas aeruginosa and Staphylococcus aureus. Antimicrob Agents Chemother 56:4360–4364. https://doi.org/10.1128/AAC.00544-12 PubMed DOI PMC

Sönmez Ş, Önal Darilmaz D, Beyatli Y (2018) Determination of the relationship between oxalate degradation and exopolysaccharide production by different Lactobacillus probiotic strains. Int J Dairy Technol 71:741–752. https://doi.org/10.1111/1471-0307.12513 DOI

Srinivasan R, Kesavelu D, Veligandla K, Muni S, Mehta S (2018) Lactobacillus reuteri DSM 17938: review of evidence in functional gastrointestinal disorders. Pediatr Ther 8:2161–2665 DOI

Sybesma W, Kort R, Lee YK (2015) Locally sourced probiotics, the next opportunity for developing countries? Trends Biotechnol 33:197–200. https://doi.org/10.1016/j.tibtech.2015.01.002 PubMed DOI

Takahashi S, Tomita J, Nishioka K, Hisada T, Nishijima M (2014) Development of a prokaryotic universal primer for simultaneous analysis of Bacteria and Archaea using next-generation sequencing. PLoS ONE 9.  https://doi.org/10.1371/journal.pone.0105592

Terpou A, Papadaki A, Lappa IK, Kachrimanidou V, Bosnea LA, Kopsahelis N (2019) Probiotics in food systems: significance and emerging strategies towards improved viability and delivery of enhanced beneficial value. Nutrients 11:32. https://doi.org/10.3390/nu11071591 DOI

Turgut T, Cakmakci S (2018) Probiotic strawberry yogurts: microbiological, chemical and sensory properties. Probiotics Antimicrob Proteins 10:64–70. https://doi.org/10.1007/s12602-017-9278-6 PubMed DOI

Turpin W, Humblot C, Noordine ML, Thomas M, Guyot JP (2012) Lactobacillaceae and cell adhesion: Genomic and functional screening. PLoS ONE 7:e38034. https://doi.org/10.1371/journal.pone.0038034 PubMed DOI PMC

Urdaneta V, Casadesús J (2017) Interactions between bacteria and bile salts in the gastrointestinal and hepatobiliary tracts. Front Med 4. https://doi.org/10.3389/fmed.2017.00163

Uriot O, Denis S, Junjua M, Roussel Y, Dary-Mourot A, Blanquet-Diot S (2017) Streptococcus thermophilus: from yogurt starter to a new promising probiotic candidate? J Funct Foods 37:74–89. https://doi.org/10.1016/j.jff.2017.07.038 DOI

Yadav AN, Yadav N, Sachan SG, Saxena AK (2019) Biodiversity of psychrotrophic microbes and their biotechnological applications. J Appl Biol Biotechnol 7:99–108. https://doi.org/10.7324/JABB.2019.70415 DOI

Yu J, Sun Z, Liu W, Bao Q, Zhang J, Zhang H (2012) Phylogenetic study of Lactobacillus acidophilus group, L. casei group and L. plantarum group based on partial hsp60, pheS and tuf gene sequences. Eur Food Res Technol 234:927–934. https://doi.org/10.1007/s00217-012-1712-0 DOI

Zhao J, Fan H, Kwok LY, Guo F, Ji R, Ya M, Chen Y (2019) Analyses of physicochemical properties, bacterial microbiota, and lactic acid bacteria of fresh camel milk collected in Inner Mongolia. J Dairy Sci 103:106–116. https://doi.org/10.3168/jds.2019-17023 PubMed DOI

Ziane M, Couvert O, Le Chevalier P, Moussa-Boudjemaa B, Leguerinel I (2016) Identification and characterization of aerobic spore forming bacteria isolated from commercial camel’s milk in south of Algeria. Small Ruminant Res 137:59–64. https://doi.org/10.1016/j.smallrumres.2016.03.004 DOI

Zielińska D, Kolożyn-Krajewska D (2018) Food-origin lactic acid bacteria may exhibit probiotic properties. Biomed Res Int 2018:16. https://doi.org/10.1155/2018/5063185 DOI

Zielińska D, Rzepkowska A, Radawska A, Zieliński K (2015) In vitro screening of selected probiotic properties of Lactobacillus strains isolated from traditional fermented cabbage and cucumber. Curr Microbiol 70:183–194. https://doi.org/10.1007/s00284-014-0699-0 PubMed DOI

Zuo F et al (2016) Characterization and in vitro properties of potential probiotic Bifidobacterium strains isolated from breast-fed infant feces. Ann Microbiol 66:1027–1037. https://doi.org/10.1007/s13213-015-1187-x DOI