Photodynamic inactivation (PDI) is a promising approach for the efficient killing of pathogenic microbes. In this study, the photodynamic effect of sulfonated polystyrene nanoparticles with encapsulated hydrophobic 5,10,15,20-tetraphenylporphyrin (TPP-NP) photosensitizers on Gram-positive (including multi-resistant) and Gram-negative bacterial strains was investigated. The cell viability was determined by the colony forming unit method. The results showed no dark cytotoxicity but high phototoxicity within the tested conditions. Gram-positive bacteria were more sensitive to TPP-NPs than Gram-negative bacteria. Atomic force microscopy was used to detect changes in the morphological properties of bacteria before and after the PDI treatment.

Department of Inorganic Chemistry Faculty of Science Charles University Prague Czech Republic

Department of Medical Biophysics Faculty of Medicine and Dentistry Palacky University in Olomouc 775 15 Olomouc Czech Republic

Department of Medical Biophysics Institute of Molecular and Translational Medicine Faculty of Medicine and Dentistry Palacky University in Olomouc Olomouc Czech Republic

Department of Microbiology Faculty of Medicine and Dentistry Palacky University in Olomouc Olomouc Czech Republic

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Biological Evaluation of Photodynamic Effect Mediated by Nanoparticles with Embedded Porphyrin Photosensitizer