Shoot branching is an important aspect of plant architecture because it substantially affects plant biology and agricultural performance. Sugars play an important role in the induction of shoot branching in several species, including potato (Solanum tuberosum L.). However, the mechanism by which sugars affect shoot branching remains mostly unknown. In the present study, we addressed this question using sugar-mediated induction of bud outgrowth in potato stems under etiolated conditions. Our results indicate that sucrose feeding to detached stems promotes the accumulation of cytokinin (CK), as well as the expression of vacuolar invertase (VInv), an enzyme that contributes to sugar sink strength. These effects of sucrose were suppressed by CK synthesis and perception inhibitors, while CK supplied to detached stems induced bud outgrowth and VInv activity in the absence of sucrose. CK-induced bud outgrowth was suppressed in vinv mutants, which we generated by genome editing. Altogether, our results identify a branching-promoting module, and suggest that sugar-induced lateral bud outgrowth is in part promoted by the induction of CK-mediated VInv activity.

Centre of the Region Haná for Biotechnological and Agricultural Research Palacký University in Olomouc Czech Republic

Department of Fruit Tree Sciences The Volcani Center ARO Rishon LeZion Israel

Department of Horticulture Michigan State University East Lansing MI 48824 USA

Department of Plant Pathology and Weed Research The Volcani Center ARO Rishon LeZion Israel

Department of Postharvest Science The Volcani Center ARO Rishon LeZion Israel

The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture The Hebrew University of Jerusalem Robert H Smith Faculty of Agriculture Food and Environment Rehovot Israel

The University of Queensland School of Biological Sciences St Lucia QLD 4072 Australia

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Plant Physiol. 2021 Apr 23;185(4):1479-1480. doi: 10.1093/plphys/kiab041. PubMed

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