Terrestrial carnivorous plants of genera Drosera, Dionaea and Nepenthes within the order Caryophyllales employ jasmonates for the induction of digestive processes in their traps. Here, we focused on two aquatic carnivorous plant genera with different trapping mechanism from distinct families and orders: Aldrovanda (Droseraceae, Caryophyllales) with snap-traps and Utricularia (Lentibulariaceae, Lamiales) with suction traps. Using phytohormone analyses and simple biotest, we asked whether the jasmonates are involved in the activation of carnivorous response similar to that known in traps of terrestrial genera of Droseraceae (Drosera, Dionaea). The results showed that Utricularia, in contrast with Aldrovanda, does not use jasmonates for activation of carnivorous response and is the second genus in Lamiales, which has not co-opted jasmonate signalling for botanical carnivory. On the other hand, the nLC-MS/MS analyses revealed that both genera secreted digestive fluid containing cysteine protease homologous to dionain although the mode of its regulation may differ. Whereas in Utricularia the cysteine protease is present constitutively in digestive fluid, it is induced by prey and exogenous application of jasmonic acid in Aldrovanda.

Department of Biochemistry Faculty of Science and Centre of the Region Haná for Biotechnological and Agricultural Research CATRIN Palacký University Šlechtitelů 27 CZ 783 71 Olomouc Czech Republic

Department of Biophysics Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Šlechtitelů 27 CZ 783 71 Olomouc Czech Republic

Institute of Botany of the Czech Academy of Sciences Department of Experimental and Functional Morphology Dukelská135 CZ 379 82 Třeboň Czech Republic

Laboratory of Growth Regulators Faculty of Science Palacký University and Institute of Experimental Botany of the Czech Academy of Sciences Šlechtitelů 27 CZ 783 71 Olomouc Czech Republic

Citace poskytuje Crossref.org

Is the co-option of jasmonate signalling for botanical carnivory a universal trait for all carnivorous plants?

Alternative or cytochrome? Respiratory pathways in traps of aquatic carnivorous bladderwort Utricularia reflexa

Cytokinins and auxins in organs of aquatic carnivorous plants: what do they reflect?

Recent ecophysiological, biochemical and evolutional insights into plant carnivory