BACKGROUND AND AIMS: Aquatic carnivorous plants have typical rootless linear shoots bearing traps and exhibit steep physiological polarity with rapid apical growth. The aim was to analyse auxin and cytokinin metabolites in traps, leaves/shoots and shoot apices in several species of genera Aldrovanda and Utricularia to elucidate how the hormonal profiles reflect the specific organ functions and polarity. METHODS: The main auxin and cytokinin metabolites were analysed in miniature samples (>2 mg dry weight) of different organs of Aldrovanda vesiculosa and six Utricularia species using ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry. KEY RESULTS: Total contents of biologically active forms (free bases, ribosides) of all four main endogenously occurring cytokinin types were consistently higher in traps than in leaves in four Utricularia species with monomorphic shoots and/or higher than in shoots in two Utricularia species with dimorphic shoots. In Aldrovanda traps, the total content of different cytokinin forms was similar to or lower than that in shoots. In U. australis leaves, feeding on prey increased all cytokinin forms, while no consistent differences occurred in Aldrovanda. In four aquatic Utricularia species with monomorphic shoots, the content of four auxin forms was usually higher in traps than in leaves. Zero IAA content was determined in U. australis leaves from a meso-eutrophic site or when prey-fed. CONCLUSIONS: Different cytokinin and auxin profiles estimated in traps and leaves/shoots of aquatic carnivorous plants indicate an association with different dominant functions of these organs: nutrient uptake by traps versus photosynthetic function of traps. Interplay of cytokinins and auxins regulates apical dominance in these plants possessing strong polarity.

Department of Chemical Biology Faculty of Science Palacký University Šlechtitelů 27 78371 Olomouc Czech Republic

Institute of Botany of the Czech Academy of Sciences Dukelská 135 CZ 379 01 Třeboň Czech Republic

Laboratory of Growth Regulators Faculty of Science Palacký University and Institute of Experimental Botany AS CR Šlechtitelů 27 78371 Olomouc Czech Republic

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Adamec L. 2000. Rootless aquatic plant DOI

Adamec L. 2009. Photosynthetic CO

Adamec L. 2011. Shoot branching of the aquatic carnivorous plant

Adamec L. 2013. A comparison of photosynthetic and respiration rates in six aquatic carnivorous DOI

Adamec L. 2014. Different reutilization of mineral nutrients in senescent leaves of aquatic and terrestrial carnivorous DOI

Adamec L. 2016. Mineral nutrition in aquatic carnivorous plants: effect of carnivory, nutrient reutilization and K DOI

Adamec L. 2018a. Ecophysiology of aquatic carnivorous plants. In: Ellison AM, Adamec L, eds. Carnivorous plants: physiology, ecology, and evolution. Oxford: Oxford University Press, 256–269.

Adamec L. 2018b. Biological flora of Central Europe: DOI

Adamec L. 2020. Biological flora of Central Europe: DOI

Bieleski RL. 1964. The problem of halting enzyme action when extracting plant tissues. Analytical Biochemistry 9: 431–442. doi: 10.1016/0003-2697(64)90204-0. PubMed DOI

Conrad K, Kohn B.. 1975. Zunahme von Cytokinin und Auxin in verwundetem Speichergewebe von DOI

Crane KE, Ross CW.. 1986. Effects of wounding on cytokinin activity in cucumber cotyledons. Plant Physiology 82: 1151–1152. doi: 10.1104/pp.82.4.1151. PubMed DOI PMC

Cross A. 2012. Aldrovanda. The waterwheel plant. Poole: Redfern Natural History Productions.

Friday LE. 1989. Rapid turnover of traps in PubMed DOI

Gu JF, Li ZK, Mao YQ, et al. 2018. Roles of nitrogen and cytokinin signals in root and shoot communications in maximizing of plant productivity and their agronomic applications. Plant Science 274: 320–331. PubMed

Guisande C, Granado-Lorencio C, Andrade-Sossa C, Duque SR.. 2007. Bladderworts. Functional Plant Science and Biotechnology 1: 58–68.

Hayat Q, Hayat S, Ali B, Ahmad A.. 2009. Auxin analogues and nitrogen metabolism, photosynthesis, and yield of chickpea. Journal of Plant Nutrition 32: 1469–1485. doi: 10.1080/01904160903092671. DOI

Hou MM, Wu DX, Li Y, Tao WQ, Chao L, Zhang YL.. 2021. The role of auxin in nitrogen-modulated shoot branching. Plant Signaling & Behavior 16: e1885888. PubMed PMC

Hoyerová K, Gaudinová A, Malbeck J, et al. 2006. Efficiency of different methods of extraction and purification of cytokinins. Phytochemistry 67: 1151–1159. doi: 10.1016/j.phytochem.2006.03.010. PubMed DOI

Hussain S, Nanda S, Zhang JH, et al. 2021. Auxin and cytokinin interplay during leaf morphogenesis and phyllotaxy. Plants 10: e1732. PubMed PMC

Jakšová J, Novák O, Adamec L, Pavlovič A.. 2021. Contrasting effect of prey capture on jasmonate signalling in two genera of aquatic carnivorous plants ( PubMed

Juniper BE, Robins RJ, Joel DM.. 1989. The carnivorous plants. London: Academic Press.

Kiba T, Kudo T, Kojima M, Sakakibara H.. 2010. Hormonal control of nitrogen acquisition: roles of auxin, abscisic acid, and cytokinin. Journal of Experimental Botany 62: 1399–1409. doi: 10.1093/jxb/erq410. PubMed DOI

Koeslin-Findeklee F, Becker MA, van der Graaff E, Roitsch T, Horst WJ.. 2015. Differences between winter oilseed rape ( PubMed DOI PMC

Lacuesta M, Saiz-Fernandez I, Podlešáková K, et al. 2018. The DOI

Miranda VFO, Silva SR, Reut MS, et al. 2021. A historical perspective of bladderworts ( PubMed DOI PMC

Novák O, Hauserová E, Amakorová P, Doležal K, Strnad M.. 2008. Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry. Phytochemistry 69: 2214–2224. doi: 10.1016/j.phytochem.2008.04.022. PubMed DOI

Pěnčík A, Rolčík J, Novák O, et al. 2009. Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction. Talanta 80: 651–655. doi: 10.1016/j.talanta.2009.07.043. PubMed DOI

Poppinga S, Bauer U, Speck T, Volkov AG.. 2018. Motile traps. In: Ellison AM, Adamec L, eds. Carnivorous plants: physiology, ecology, and evolution. Oxford: Oxford University Press, 180–193.

Richards JH. 2001. Bladder function in PubMed DOI

Sano H, Seo S, Orudgev E, et al. 1994. Expression of the gene for a small GTP binding protein in transgenic tobacco elevates endogenous cytokinin levels, abnormally induces salicylic acid in response to wounding, and increases resistance to tobacco mosaic virus infection. Proceedings of the National Academy of Sciences of the USA 91: 10556–10560. PubMed PMC

Shimizu-Sato S, Tanaka M, Mori H.. 2009. Auxin-cytokinin interactions in the control of shoot branching. Plant Molecular Biology 69: 429–435. PubMed

Silva-Navas J, Conesa CM, Saez A, et al. 2019. Role of cis-zeatin in root responses to phosphate starvation. New Phytologist 224: 242–257. PubMed

Šimura J, Spíchal L, Adamec L, et al. 2016. Cytokinin, auxin and physiological polarity in the aquatic carnivorous plants PubMed DOI PMC

Sirová D, Adamec L, Vrba J.. 2003. Enzymatic activities in traps of four aquatic species of the carnivorous genus PubMed DOI

Sirová D, Borovec J, Černá B, Rejmánková E, Adamec L, Vrba J.. 2009. Microbial community development in the traps of aquatic

Smigocki AC. 1995. Expression of a wound-inducible cytokinin biosynthesis gene in transgenic tobacco: correlation of root expression with induction of cytokinin effects. Plant Science 109: 153–163. doi: 10.1016/0168-9452(94)04157-c. DOI

Sun D, Zhang L, Yu Q, et al. 2021. Integrated signals of jasmonates, sugars, cytokinins and auxin influence the initial growth of the second buds of chrysanthemum after decapitation. Biology 10: 440e440. doi: 10.3390/biology10050440. PubMed DOI PMC

Svačinová J, Novák O, Plačková L, et al. 2012. A new approach for cytokinin isolation from PubMed PMC

Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H.. 2006. Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant Journal 45: 1028–1036. PubMed

Tarkowská D, Novák O, Floková K, et al. 2014. Quo vadis plant hormone analysis? Planta 240: 55–76. doi: 10.1007/s00425-014-2063-9. PubMed DOI

Taylor P. 1989.

Werner T, Holst K, Pörs Y, et al. 2008. Cytokinin deficiency causes distinct changes of sink and source parameters in tobacco shoots and roots. PubMed DOI PMC

Hormonal profiles in dormant turions of 22 aquatic plant species: do they reflect functional or taxonomic traits?

Characteristics of turion development in two aquatic carnivorous plants: Hormonal profiles, gas exchange and mineral nutrient content