We present the structural modification of a commercially available glass ionomer cement by inserting the imidazolium salt 1-n-hexadecyl-3-methylimidazolium chloride (C16MImCl), composing a new biomaterial with antifungal biofilm activity. Test specimens were prepared using a commercial glass ionomer cement to which 10 ppm of cetylpyridinium chloride (reference ionic antifungal agent) or C16MImCl were added. The feasibility and hypoallergenicity of the new biomaterial were assessed by microhardness plastic deformation and chorioallantoic membrane assays. Colony counting and scanning electron microscopy were used to evaluate the modified specimens' antibiofilm activity against three multidrug-resistant Candida species. The modified glass ionomer cement presented a strong antibiofilm activity against Candida spp., without losing its original micromechanical and hypoallergenic properties, rendering it a promising candidate for further application in dentistry.

Biotechnology Center UFRGS Porto Alegre RS 91501 970 Brazil

Institute of Macromolecular Chemistry Czech Academy of Sciences Prague Czech Republic

Laboratory of Applied Mycology UFRGS Porto Alegre RS 90610 000 Brazil

Laboratory of Biomedical Science Universidade Luterana Do Brasil Br 285 km 335 Carazinho RS 99500 000 Brazil

Postgraduate Program in Biological Sciences Pharmacology and Therapeutics Universidade Federal Do Rio Grande Do Sul Porto Alegre RS 90050 170 Brazil

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