The evolutionarily conserved Swi5-Sfr1 complex plays an important role in homologous recombination, a process crucial for the maintenance of genomic integrity. Here, we purified Schizosaccharomyces pombe Swi5-Sfr1 complex from meiotic cells and analyzed it by mass spectrometry. Our analysis revealed new phosphorylation sites on Swi5 and Sfr1. We found that mutations that prevent phosphorylation of Swi5 and Sfr1 do not impair their function but swi5 and sfr1 mutants encoding phosphomimetic aspartate at the identified phosphorylation sites are only partially functional. We concluded that during meiosis, Swi5 associates with Sfr1 and both Swi5 and Sfr1 proteins are phosphorylated. However, the functional relevance of Swi5 and Sfr1 phosphorylation remains to be determined.

Advanced Microscopy Facility VBCF and Department of Chromosome Biology Max Perutz Labs University of Vienna Vienna Biocenter Dr Bohr Gasse 9 1030 Vienna Austria

Cancer Research Institute Biomedical Research Center of the Slovak Academy of Sciences Dubravska Cesta 9 845 05 Bratislava Slovakia

Department of Genetics Cancer Research Institute Biomedical Research Center Slovak Academy of Sciences Dubravska Cesta 9 845 05 Bratislava Slovakia

Department of Genetics Faculty of Natural Sciences Comenius University in Bratislava Ilkovicova 6 842 15 Bratislava Slovakia

Institute of Molecular Genetics of the Czech Academy of Sciences Videnska 1083 142 20 Prague 4 Czech Republic

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