OBJECTIVES: As a liquid organic fertilizer used in agriculture, digestate is rich in many nutrients (i.e. nitrogen, phosphorus, sulfur, calcium, potassium); their utilization may be however less efficient in soils poor in organic carbon (due to low carbon:nitrogen ratio). In order to solve the disadvantages, digestate enrichment with carbon-rich amendments biochar or humic acids (Humac) was tested. METHODS: Soil variants amended with enriched digestate: digestate + biochar, digestate + Humac, and digestate + combined biochar and humic acids-were compared to control with untreated digestate in their effect on total soil carbon and nitrogen, microbial biomass carbon, soil respiration and soil enzymatic activities in a pot experiment. Yield of the test crop lettuce was also determined for all variants. RESULTS: Soil respiration was the most significantly increased property, positively affected by digestate + Humac. Both digestate + biochar and digestate + Humac significantly increased microbial biomass carbon. Significant negative effect of digestate + biochar (compared to the control digestate) on particular enzyme activities was alleviated by the addition of humic acids. No significant differences among the tested variants were found in the above-ground and root plant biomass. CONCLUSIONS: The tested organic supplements improved the digestate effect on some determined soil properties. We deduced from the results (carbon:nitrogen ratio, microbial biomass and activity) that the assimilation of nutrients by plants increased; however, the most desired positive effect on the yield of crop biomass was not demonstrated. We assume that the digestate enrichment with organic amendments may be more beneficial in a long time-scaled trial.

Agricultural Research Ltd Troubsko Czech Republic

Department of Agrochemistry Soil Science Microbiology and Plant Nutrition Faculty of AgriSciences Mendel University in Brno Brno Czech Republic

Department of Agronomy Faculty of Agricultural Sciences and Technology Bahauddin Zakariya University Multan Punjab Pakistan

Department of Agronomy the University of Haripur Khyber Pakhtunkhwa Pakistan

Department of Geology and Soil Science Faculty of Forestry and Wood Technology Mendel University in Brno Brno Czech Republic

Department of Soil Science and Plant Nutrition Faculty of Agriculture Ankara University Ankara Turkey

Department of Soil Science Faculty of Agricultural Sciences and Technology Bahauddin Zakariya University Multan Punjab Pakistan

Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource College of Tropical Crops Hainan University Haikou China

Institute of Chemistry and Technology of Environmental Protection Faculty of Chemistry Brno University of Technology Brno Czech Republic

Odvolání publikace

PLoS One. 2022 Sep 14;17(9):e0274148. doi: 10.1371/journal.pone.0274148. PubMed

Zobrazit více v PubMed

Masse DI, Talbot G, Gilbert Y. On farm biogas production: A method to reduce GHG emissions and develop more sustainable livestock operations. Anim Feed Sci Technol. 2011; 166–67: 436–445.

Liedl BE, Bombardiere J, Williams ML, Stowers A, Postalwait C, Chatfield JM. Solid Effluent from Thermophilic Anaerobic Digestion of Poultry Litter as a Potential Fertilizer. HortScience. 2004; 39(4): 877B–877.

Garg RN, Pathak H, Das DK, Tomar RK. Use of flyash and biogas slurry for improving wheat yield and physical properties of soil. Environ Monit Assess. 2005; 107(1–3): 1–9. doi: 10.1007/s10661-005-2021-x PubMed DOI

Erhart E, Siegl T, Bonell M, Unterfrauner H, Peticzka R, Ableidinger C, et al.. Fertilization with liquid digestate in organic farming—effects on humus balance, soil potassium contents and soil physical properties. EGU General Assembly, Vienna, Austria. 2014; id.16.4419E

Galvez A, Sinicco T, Cayuela ML, Mingorance MD, Fornasier F, Mondini C. Short term effects of bioenergy by-products on soil C and N dynamics, nutrient availability and biochemical properties. Agric Ecosyst Environ. 2012; 160: 3–14.

Möller K. Systemwirkungen einer "Biogaswirtschaft" im ökologischen Landbau: Pflanzenbauliche Aspekte, Auswirkungen auf den N-Haushalt und auf die Spurengasemissionen. In: Fachverband Biogas eV, editor.: bigben: Verlag; 2003. pp. 20–29.

Khan Z, Zhang K, Khan MN, Fahad S, Xu Z, Hu L. Coupling of Biochar with Nitrogen Supplements Improve Soil Fertility, Nitrogen Utilization Efficiency and Rapeseed Growth. Agronomy-Basel. 2020; 10(11): 1661.

Breunig H, Amirebrahimi J, Smith S, Scown C. Role of Digestate and Biochar in Carbon-Negative Bioenergy. Environ. Sci. Technol. 2019; 53 (22): 12989–12998 doi: 10.1021/acs.est.9b03763 PubMed DOI

Chen J, Jiang X, Tang X, Sun Y, Zhou L. Use of biochar/persulfate for accelerating the stabilization process and improving nitrogen stability of animal waste digestate. Sci Total Environ. 2021; 757: 144158. doi: 10.1016/j.scitotenv.2020.144158 PubMed DOI

Suddapuli Hewage RP. Effect of charred digestate (biochar) and digestate on soil organic carbon and nutrients in temperate bioenergy crop production systems. Doctoral Thesis, Universität Hamburg. 2016. Available from: https://ediss.sub.uni-hamburg.de/handle/ediss/6679

Cardelli R, Giussani G, Marchini F, Saviozzi A. Short-term effects on soil of biogas digestate, biochar and their combinations. Soil Research. 2018; 56 (6): 623–631.

Kizito S, Luo H, Lu J, Bah H, Dong R, Wu S. Role of Nutrient-Enriched Biochar as a Soil Amendment during Maize Growth: Exploring Practical Alternatives to Recycle Agricultural Residuals and to Reduce Chemical Fertilizer Demand. 2019; 11(11): 3211.

Udall D, Rayns F. The potential of Biochar and Anaerobic Digestate use in a Temperate Conventional Wheat Production System. International Journal of Research in Agriculture and Forestry. 2017; 4(10): 44–49.

Li H, Li Y, Li C. Evolution of humic substances during anaerobic sludge digestion. Environmental engineering and management journal. 2017; 16 (7): 1577–1582.

Pospisilova L, Horakova E, Fisera M, Jerzykiewicz M, Mensik L. Effect of selected organic materials on soil humic acids chemical properties. Environmental research. 2020; 187: 109663. doi: 10.1016/j.envres.2020.109663 PubMed DOI

Leogrande R, Lopedota O, Vitti C, Ventrella D, Montemurro F. Effects of irrigation volumes and organic fertilizers on eggplant grown in Mediterranean environment. Acta Agriculturae Scandinavica Section B-Soil and Plant Science. 2014; 64(6): 518–528.

Manasa MRK, Katukuri NR, Xu XH, Guo RB. Rehabilitation of saline soil with biogas digestate, humic acid, calcium humate and their amalgamations. Commun Soil Sci Plant Anal. 2020; 51(13): 1707–1724.

Holatko J, Hammerschmiedt T, Datta R, Baltazar T, Kintl A, Latal O, et al.. Humic Acid Mitigates the Negative Effects of High Rates of Biochar Application on Microbial Activity. Sustainability. 2020; 12(22): 9524.

Przygocka-Cyna K, Grzebisz W, Biber M. Evaluation of the potential of bio-fertilizers as a source of nutrients and heavy metals by means of the exhaustion lettuce test. Journal of Elementology. 2018; 23(2): 429–445.

Bankole A, Umebese CE, Feyisola RT, Bamise O. Influence of salicylic acid on the growth of lettuce (Lactuca sativa var longifolia) during reduced leaf water potential. Journal of Applied Sciences and Environmental Management. 2018; 22(4): 543–540.

Zhang T, Shi Y, Piao F, Sun Z. Effects of different LED sources on the growth and nitrogen metabolism of lettuce. Plant Cell, Tissue and Organ Culture (PCTOC). 2018; 134(2): 231–240.

Chrysargyris A, Xylia P, Anastasiou M, Pantelides I, Tzortzakis N. Effects of Ascophyllum nodosum seaweed extracts on lettuce growth, physiology and fresh-cut salad storage under potassium deficiency. Journal of the Science of Food and Agriculture. 2018; 98(15): 5861–5872. doi: 10.1002/jsfa.9139 PubMed DOI

Cervera-Mata A, Pastoriza S, Rufián-Henares JÁ, Párraga J, Martín-García JM, Delgado G. Impact of spent coffee grounds as organic amendment on soil fertility and lettuce growth in two Mediterranean agricultural soils. Arch Agron Soil Sci. 2018; 64(6): 790–804.

Iocoli GA, Zabaloy MC, Pasdevicelli G, Gómez MA. Use of biogas digestates obtained by anaerobic digestion and co-digestion as fertilizers: Characterization, soil biological activity and growth dynamic of Lactuca sativa L. Sci Total Environ. 2019; 647: 11–19. doi: 10.1016/j.scitotenv.2018.07.444 PubMed DOI

Trinchera A, Baratella V, Rinaldi S, Renzaglia M, Marcucci A, Rea E. Greenhouse Lettuce: Assessing Nutrient Use Efficiency Of Digested Livestock Manure As Organic N-Fertilizer. Conference: II International Symposium on Organic Greenhouse Horticulture OGH (2013). 2018.

ISO 10694:1995. Soil quality—Determination of organic and total carbon after dry combustion (Elemental analysis). 2016.

ISO 13878:1998. Soil quality—Determination of total nitrogen content by dry combustion (Elemental analysis). 2020.

Vance ED, Brookes PC, Jenkinson DS. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry. 1987; 19(6): 703–707.

Campbell CD, Chapman SJ, Cameron CM, Davidson MS, Potts JM. A Rapid Microtiter Plate Method To Measure Carbon Dioxide Evolved from Carbon Substrate Amendments so as To Determine the Physiological Profiles of Soil Microbial Communities by Using Whole Soil. Applied and Environmental Microbiology. 2003; 69(6): 3593–3599. doi: 10.1128/AEM.69.6.3593-3599.2003 PubMed DOI PMC

ISO 20130:2018. Soil quality—Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates. 2018.

Tabatabai MA. Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. Page AL, editor. Madison, WI: American Society of Agronomy, Soil Science Society of America. 1982.

Voberkova S, Vaverkova MD, Buresova A, Adamcova D, Vrsanska M, Kynicky J, et al.. Effect of inoculation with white-rot fungi and fungal consortium on the composting efficiency of municipal solid waste. Waste management (New York, NY). 2017; 61: 157–64. PubMed

Hinkle DE, Wiersma W, Jurs SG. Applied statistics for the behavioral sciences. 5th ed. Boston: Houghton Mifflin; 2003.

Haefele SM, Konboon Y, Wongboon W, Amarante S, Maarifat AA, Pfeiffer EM, et al.. Effects and fate of biochar from rice residues in rice-based systems. Field Crops Research. 2011; 121(3): 430–440.

Shahbaz AK, Ramzani PMA, Saeed R, Turan V, Iqbal M, Lewińska K, et al.. Effects of biochar and zeolite soil amendments with foliar proline spray on nickel immobilization, nutritional quality and nickel concentrations in wheat. Ecotoxicol Environ Saf. 2019;173: 182–191. doi: 10.1016/j.ecoenv.2019.02.025 PubMed DOI

Bamminger C, Zaiser N, Zinsser P, Lamers M, Kammann C, Marhan S. Effects of biochar, earthworms, and litter addition on soil microbial activity and abundance in a temperate agricultural soil. Biol Fertil Soils. 2014; 50(8): 1189–1200.

Foster EJ, Fogle EJ, Cotrufo MF. Sorption to Biochar Impacts β-Glucosidase and Phosphatase Enzyme Activities. Agriculture. 2018; 8(10): 158.

Karaca A, Turgay OC, Tamer N. Effects of a humic deposit (gyttja) on soil chemical and microbiological properties and heavy metal availability. Biol Fertil Soils. 2006; 42(6): 585–592.

Dehsheikh AB, Sourestani MM, Zolfaghari M, Enayatizamir N. Changes in soil microbial activity, essential oil quantity, and quality of Thai basil as response to biofertilizers and humic acid. J Clean Prod. 2020; 256: 120439.

Plaimart J, Acharya K, Mrozik W, Davenport RJ, Vinitnantharat S, Werner D. Coconut husk biochar amendment enhances nutrient retention by suppressing nitrification in agricultural soil following anaerobic digestate application. Environ Pollut. 2021; 268(Pt A): 115684. doi: 10.1016/j.envpol.2020.115684 PubMed DOI PMC

Tambone F, Adani F. Nitrogen mineralization from digestate in comparison to sewage sludge, compost and urea in a laboratory incubated soil experiment. J Plant Nutr Soil Sci. 2017; 180(3): 355–365.

Yao Y, Gao B, Zhang M, Inyang M, Zimmerman AR. Effect of biochar amendment on sorption and leaching of nitrate, ammonium, and phosphate in a sandy soil. Chemosphere. 2012; 89(11): 1467–1471. doi: 10.1016/j.chemosphere.2012.06.002 PubMed DOI

Nasim W, Ahmad A, Amin A, Tariq M, Awais M, Saqib M, et al.. Radiation efficiency and nitrogen fertilizer impacts on sunflower crop in contrasting environments of Punjab, Pakistan. Environ Sci Pollut Res. 2018; 25: 1822–1836 doi: 10.1007/s11356-017-0592-z PubMed DOI

Dong L, Cordova-Kreylos AL, Yang J, Yuan H, Scow KM. Humic acids buffer the effects of urea on soil ammonia oxidizers and potential nitrification. Soil Biol Biochem. 2009; 41(8): 1612–1621. doi: 10.1016/j.soilbio.2009.04.023 PubMed DOI PMC

Klammsteiner T, Turan V, Fernández-Delgado Juárez M, Oberegger S, Insam H. Suitability of Black Soldier Fly Frass as Soil Amendment and Implication for Organic Waste Hygienization. Agronomy. 2020; 10(10): 1578.

Reibe K, Ross CL, Ellmer F. Hydro-/Biochar application to sandy soils: impact on yield components and nutrients of spring wheat in pots. Arch Agron Soil Sci. 2015; 61(8): 1055–1060.

Khan MA, Mahmood-ur-Rahman, Ramzani PMA, Zubair M, Rasool B, Khan MK, et al.. Associative effects of lignin-derived biochar and arbuscular mycorrhizal fungi applied to soil polluted from Pb-acid batteries effluents on barley grain safety. Sci Total Environ. 2020; 710: 136294. doi: 10.1016/j.scitotenv.2019.136294 PubMed DOI

Naeem I, Masood N, Turan V, Iqbal M. Prospective usage of magnesium potassium phosphate cement combined with Bougainvillea alba derived biochar to reduce Pb bioavailability in soil and its uptake by Spinacia oleracea L. Ecotoxicol Environ Saf. 2021; 208: 111723. doi: 10.1016/j.ecoenv.2020.111723 PubMed DOI

Turan V. Confident performance of chitosan and pistachio shell biochar on reducing Ni bioavailability in soil and plant plus improved the soil enzymatic activities, antioxidant defense system and nutritional quality of lettuce. Ecotoxicol Environ Saf. 2019; 183: 109594. doi: 10.1016/j.ecoenv.2019.109594 PubMed DOI

Zubair M, Ramzani PMA, Rasool B, Khan MA, Akhtar I, Turan V, et al.. Efficacy of chitosan-coated textile waste biochar applied to Cd-polluted soil for reducing Cd mobility in soil and its distribution in moringa (Moringa oleifera L.). J Environ Manage. 2021; 284: 112047. doi: 10.1016/j.jenvman.2021.112047 PubMed DOI

Effect of Mycotoxins in Silage on Biogas Production