Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34348153
DOI
10.1016/j.celrep.2021.109484
PII: S2211-1247(21)00911-6
Knihovny.cz E-resources
- Keywords
- DSS, Single cell RNA sequencing, colitis, colon development, colon inflammatory disease, hindgut development, intestinal damage, intestinal development, single-cell atlas,
- MeSH
- Single-Cell Analysis MeSH
- Cell Differentiation MeSH
- Embryo, Mammalian metabolism MeSH
- Inflammatory Bowel Diseases genetics pathology MeSH
- Colitis genetics MeSH
- Colon embryology pathology MeSH
- Humans MeSH
- Mesoderm embryology MeSH
- Disease Models, Animal MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Gene Expression Profiling * MeSH
- Intestinal Mucosa embryology metabolism pathology MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We lack a holistic understanding of the genetic programs orchestrating embryonic colon morphogenesis and governing damage response in the adult. A window into these programs is the transcriptomes of the epithelial and mesenchymal cell populations in the colon. Performing unbiased single-cell transcriptomic analyses of the developing mouse colon at different embryonic stages (embryonic day 14.5 [E14.5], E15.5, and E18.5), we capture cellular and molecular profiles of the stages before, during, and after the appearance of crypt structures, as well as in a model of adult colitis. The data suggest most adult lineages are established by E18.5. We find embryonic-specific gene expression profiles and cell populations that reappear in response to tissue damage. Comparison of the datasets from mice and human colitis suggests the processes are conserved. In this study, we provide a comprehensive single-cell atlas of the developing mouse colon and evidence for the reactivation of embryonic genes in disease.
References provided by Crossref.org
Terminal differentiation of villus tip enterocytes is governed by distinct Tgfβ superfamily members
