Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes
Language English Country Switzerland Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34439734
PubMed Central
PMC8393889
DOI
10.3390/biom11081068
PII: biom11081068
Knihovny.cz E-resources
- Keywords
- adipogenesis, human adipose-derived mesenchymal stem cells, mitochondrial respiration, p,p′-DDE,
- MeSH
- Adipogenesis drug effects MeSH
- Cell Differentiation drug effects MeSH
- Dichlorodiphenyl Dichloroethylene toxicity MeSH
- Cells, Cultured MeSH
- Environmental Pollutants toxicity MeSH
- Humans MeSH
- Membrane Potential, Mitochondrial MeSH
- Mesenchymal Stem Cells cytology drug effects MeSH
- Mitochondria drug effects MeSH
- Obesity metabolism MeSH
- Adipocytes cytology drug effects MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dichlorodiphenyl Dichloroethylene MeSH
- Environmental Pollutants MeSH
The contribution of environmental pollutants to the obesity pandemic is still not yet fully recognized. Elucidating possible cellular and molecular mechanisms of their effects is of high importance. Our study aimed to evaluate the effect of chronic, 21-day-long, 2,2-bis (4-chlorophenyl)-1,1-dichlorethylenedichlorodiphenyldichloroethylene (p,p'-DDE) exposure of human adipose-derived mesenchymal stem cells committed to adipogenesis on mitochondrial oxygen consumption on days 4, 10, and 21. In addition, the mitochondrial membrane potential (MMP), the quality of the mitochondrial network, and lipid accumulation in maturing cells were evaluated. Compared to control differentiating adipocytes, exposure to p,p'-DDE at 1 μM concentration significantly increased basal (routine) mitochondrial respiration, ATP-linked oxygen consumption and MMP of intact cells on day 21 of adipogenesis. In contrast, higher pollutant concentration seemed to slow down the gradual increase in ATP-linked oxygen consumption typical for normal adipogenesis. Organochlorine p,p'-DDE did not alter citrate synthase activity. In conclusion, in vitro 1 μM p,p'-DDE corresponding to human exposure is able to increase the mitochondrial respiration per individual mitochondrion at the end of adipocyte maturation. Our data reveal that long-lasting exposure to p,p'-DDE could interfere with the metabolic programming of mature adipocytes.
Biomedical Centre Faculty of Medicine in Pilsen Charles University 301 00 Pilsen Czech Republic
Department of Biology Faculty of Medicine in Pilsen Charles University 301 00 Pilsen Czech Republic
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