Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34742912
DOI
10.1016/j.bbapap.2021.140735
PII: S1570-9639(21)00141-2
Knihovny.cz E-resources
- Keywords
- FPOP, Footprinting, Haptoglobin, Hemoglobin, timsTOF pro,
- MeSH
- Amino Acids chemistry metabolism MeSH
- Protein Footprinting methods MeSH
- Haptoglobins chemistry metabolism MeSH
- Hemoglobins chemistry metabolism MeSH
- Mass Spectrometry methods MeSH
- Hydroxyl Radical chemistry MeSH
- Humans MeSH
- Models, Molecular MeSH
- Molecular Structure MeSH
- Oxidation-Reduction MeSH
- Protein Binding MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- haptoglobin-hemoglobin complex MeSH Browser
- Haptoglobins MeSH
- Hemoglobins MeSH
- Hydroxyl Radical MeSH
Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single residue resolution. In the present study, FPOP analysis was applied to study the hemoglobin (Hb) - haptoglobin (Hp) complex allowing identification of respective regions altered upon the complex formation. FPOP footprinting using a timsTOF Pro mass spectrometer revealed structural information for 84 and 76 residues in Hp and Hb, respectively, including statistically significant differences in the modification extent below 0.3%. The most affected residues upon complex formation were Met76 and Tyr140 in Hbα, and Tyr280 and Trp284 in Hpβ. The data allowed determination of amino acids directly involved in Hb - Hp interactions and those located outside of the interaction interface yet affected by the complex formation. Also, previously modeled interaction between Hb βTrp37 and Hp βPhe292 was not confirmed by our data. Data are available via ProteomeXchange with identifier PXD021621.
BioCeV Institute of Microbiology of the CAS Prumyslova 595 CZ 252 50 Vestec Czech Republic
Bruker Daltonik GmbH Fahrenheitstraße 4 28359 Bremen Germany
References provided by Crossref.org
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