Hydroxyl radical footprinting analysis of a human haptoglobin-hemoglobin complex
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
34742912
DOI
10.1016/j.bbapap.2021.140735
PII: S1570-9639(21)00141-2
Knihovny.cz E-zdroje
- Klíčová slova
- FPOP, Footprinting, Haptoglobin, Hemoglobin, timsTOF pro,
- MeSH
- aminokyseliny chemie metabolismus MeSH
- footprinting proteinů metody MeSH
- haptoglobiny chemie metabolismus MeSH
- hemoglobiny chemie metabolismus MeSH
- hmotnostní spektrometrie metody MeSH
- hydroxylový radikál chemie MeSH
- lidé MeSH
- molekulární modely MeSH
- molekulární struktura MeSH
- oxidace-redukce MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aminokyseliny MeSH
- haptoglobin-hemoglobin complex MeSH Prohlížeč
- haptoglobiny MeSH
- hemoglobiny MeSH
- hydroxylový radikál MeSH
Methods of structural mass spectrometry have become more popular to study protein structure and dynamics. Among them, fast photochemical oxidation of proteins (FPOP) has several advantages such as irreversibility of modifications and more facile determination of the site of modification with single residue resolution. In the present study, FPOP analysis was applied to study the hemoglobin (Hb) - haptoglobin (Hp) complex allowing identification of respective regions altered upon the complex formation. FPOP footprinting using a timsTOF Pro mass spectrometer revealed structural information for 84 and 76 residues in Hp and Hb, respectively, including statistically significant differences in the modification extent below 0.3%. The most affected residues upon complex formation were Met76 and Tyr140 in Hbα, and Tyr280 and Trp284 in Hpβ. The data allowed determination of amino acids directly involved in Hb - Hp interactions and those located outside of the interaction interface yet affected by the complex formation. Also, previously modeled interaction between Hb βTrp37 and Hp βPhe292 was not confirmed by our data. Data are available via ProteomeXchange with identifier PXD021621.
BioCeV Institute of Microbiology of the CAS Prumyslova 595 CZ 252 50 Vestec Czech Republic
Bruker Daltonik GmbH Fahrenheitstraße 4 28359 Bremen Germany
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