Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
34826706
DOI
10.1016/j.plaphy.2021.11.019
PII: S0981-9428(21)00579-9
Knihovny.cz E-zdroje
- Klíčová slova
- Anaesthesia, Arabidopsis thaliana, Calcium, Electrical signal, Heat wounding, Jasmonic acid,
- MeSH
- anestetika * MeSH
- Arabidopsis * genetika metabolismus MeSH
- cyklopentany farmakologie MeSH
- ether MeSH
- listy rostlin metabolismus MeSH
- oxylipiny farmakologie MeSH
- proteiny huseníčku * genetika metabolismus MeSH
- regulace genové exprese u rostlin MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anestetika * MeSH
- cyklopentany MeSH
- ether MeSH
- jasmonic acid MeSH Prohlížeč
- oxylipiny MeSH
- proteiny huseníčku * MeSH
General volatile anaesthetics (GVA) inhibit electrical signal propagation in animal neurons. Although plants do not have neurons, they generate and propagate electrical signals systemically from a local damaged leaf to neighbouring leaves. This systemic electrical signal propagation is mediated by ligand-gated glutamate receptor-like (GLR) channels. Here, we investigated the effect of GVA diethyl ether on the systemic electrical and further downstream responses in Arabidopsis thaliana. We monitored electrical signals, cytoplasmic Ca2+ level ([Ca2+]cyt), ultra-weak photon emission, amino acid contents, phytohormone response as well as gene expression in response to heat wounding during diethyl ether anaesthesia. We found complete suppression of electrical and [Ca2+]cyt signal propagation from damaged leaf to neighbouring systemic leaves upon diethyl ether treatment. Concomitantly, jasmonates (JAs) did not accumulate and expression of JA-responsive genes (AOS, OPR3, JAZ10) was not detected in systemic leaves. However local damaged leaves still showed increased [Ca2+]cyt and accumulated high level of JAs and JA-inducible transcripts. An exogenously added GLR ligand, L-glutamate, was not able to trigger Ca2+ wave in etherized plants indicating that GLRs are targeted by diethyl ether, but not specifically. The fact that GVA inhibit electrical signal propagation not only in animals but also in plants is intriguing. However, the cellular response is completely blocked only in systemic leaves; the local damaged leaf still senses damaging stimuli.
Citace poskytuje Crossref.org
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