BACKGROUND: Recurrent aphthous stomatitis (RAS) is multifactorial disease with unclear etiopathogenesis. The aim of this study was to determine distribution of the angiotensin I converting enzyme (ACE) gene polymorphisms and their influence on RAS susceptibility in Czech population. METHODS: The study included 230 subjects (143 healthy controls and 87 patients with RAS) with anamnestic, clinical and laboratory data. Five ACE gene polymorphisms (rs4291/rs4305/rs4311/rs4331/rs1799752 = ACE I/D) were determined by TaqMan technique. RESULTS: The allele and genotype distributions of the studied ACE I/D polymorphisms were not significantly different between subjects with/without RAS (Pcorr > 0.05). However, carriers of II genotype were less frequent in the RAS group (OR = 0.48, 95% CI = 0.21-1.12, P = 0.059). Stratified analysis by sex demonstrated lower frequency of II genotype in women (OR = 0.33, 95% CI = 0.09-1.17, P < 0.035, Pcorr > 0.05, respectively) than in men with RAS (P > 0.05). Moreover, the frequency of AGTGD haplotype was significantly increased in RAS patients (OR = 13.74, 95% CI = 1.70-110.79, P = 0.0012, Pcorr < 0.05). In subanalysis, TGD haplotype was significantly more frequent in RAS patients (P < 0.00001) and CGI haplotype was less frequent in RAS patients (P < 0.01), especially in women (P = 0.016, Pcorr > 0.05). CONCLUSIONS: Our study indicates that while the AGTGD and TGD haplotypes are associated with increased risk of RAS development, CGI haplotype might be one of protective factors against RAS susceptibility in Czech population.

Clinic of Stomatology Institution Shared with St Anne's University Hospital Faculty of Medicine Masaryk University Pekarska 664 53 60200 Brno Czech Republic

Czech National Centre for Evidence Based Healthcare and Knowledge Translation Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Kamenice 753 5 62500 Brno Czech Republic

Department of Biochemistry Faculty of Science Masaryk University Kamenice 753 5 62500 Brno Czech Republic

Department of Clinical Immunology and Allergology Institution Shared with St Anne's Faculty Hospital and Faculty of Medicine Masaryk University Pekarska 664 53 60200 Brno Czech Republic

Department of Pathophysiology Faculty of Medicine Masaryk University Kamenice 753 5 62500 Brno Czech Republic

Department of Stomatology General University Hospital Prague and 1st Faculty of Medicine Charles University Karlovo nam 554 32 12808 Prague Czech Republic

Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Kamenice 753 5 62500 Brno Czech Republic

Institute of Immunology and Microbiology General University Hospital Prague and 1st Faculty of Medicine Charles University Karlovo nam 554 32 Prague Czech Republic

RECETOX Faculty of Science Masaryk University Kotlarska 2 61137 Brno Czech Republic

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