Phytophthora infections are followed by histological alterations, physiological and metabolomic adjustments in the host but very few studies contemplate these changes simultaneously. Fagus sylvatica seedlings were inoculated with A1 and A2 mating types of the heterothallic P. ×cambivora and with the homothallic P. plurivora to identify plant physiological and metabolomic changes accompanying microscope observations of the colonization process one, two and three weeks after inoculation. Phytophthora plurivora-infected plants died at a faster pace than those inoculated with P. ×cambivora and showed higher mortality than P. ×cambivora A1-infected plants. Phytophthora ×cambivora A1 and A2 caused similar progression and total rate of mortality. Most differences in the physiological parameters between inoculated and non-inoculated plants were detected two weeks after inoculation. Alterations in primary and secondary metabolites in roots and leaves were demonstrated for all the inoculated plants two and three weeks after inoculation. The results indicate that P. plurivora is more aggressive to Fagus sylvatica seedlings than both mating types of P. ×cambivora while P. ×cambivora A1 showed a slower infection mode than P. ×cambivora A2 and led to minor plant metabolomic adjustments.

Department of Agricultural Sciences University of Sassari Viale Italia 39 07100 Sassari Italy

Department of Forest Botany Dendrology and Geobiocoenology Faculty of Forestry and Wood Technology Mendel University in Brno Zemědělská 3 61300 Brno Czech Republic

Department of Forest Protection and Wildlife Management Faculty of Forestry and Wood Technology Mendel University in Brno Zemědělská 3 61300 Brno Czech Republic

Department of Phytology Technical University in Zvolen 96001 Zvolen Slovakia

Department of Wood Science Technical University in Zvolen 96001 Zvolen Slovakia

Faculty of Forestry University of Belgrade Kneza Višeslava 1 11030 Belgrade Serbia

Institute of Botany Technische Universität Dresden 01062 Dresden Germany

Phytophthora Research and Consultancy Am Rain 9 83131 Nußdorf Germany

Phytophthora Research Centre Faculty of Forestry and Wood Technology Mendel University in Brno 61300 Brno Czech Republic

Phytophthora Research Centre Laboratory of Molecular Biology and Radiobiology Faculty of AgriSciences Mendel University in Brno Zemědělská 1 61300 Brno Czech Republic

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