Cercarial activity and survival are crucial traits for the transmission of trematodes. Temperature is particularly important, as faster depletion of limited cercarial energy reserves occurs at high temperatures. Seasonal climate conditions in high latitude regions may be challenging to complete trematode life cycle during the 6-month ice-free period, but temperature effects on the activity and survival of freshwater cercariae have not been previously identified. After experimentally simulating natural subarctic conditions during warmer and colder months (13 and 6°C), a statistical approach identifying changes in the tendency of cercarial activity loss and mortality data was used to detect differences in three trematode genera, represented by four taxa (Diplostomum spp., Apatemon spp., small- and large-sized Plagiorchis spp.). A strong temperature-dependent response was identified in both activity loss and mortality in all taxa, with Diplostomum spp. cercariae showing the most gradual changes compared to other taxa. Furthermore, whilst activity loss and mortality dynamics could not be divided into ‘fish- vs invertebrate-infecting cercariae’ groups, the detected taxa-specific responses in relation to life-history traits indicate the swimming behaviour of cercariae and energy allocation among larvae individuals as the main drivers. Cercariae exploit the short transmission window that allows a stable continuance of trematodes’ life cycles in high-latitude freshwater ecosystems.

Cavanilles Institute for Biodiversity and Evolutionary Biology Science Park University of Valencia P O Box 22085 46071Valencia Spain

Department of Arctic and Marine Biology Faculty of Biosciences Fisheries and Economics UiT The Arctic University of Norway N9037Tromsø Norway

Department of Coastal Systems NIOZ Royal Netherlands Institute for Sea Research P O Box 59 1790 AB Den Burg Texel The Netherlands

Faculty of Science University of South Bohemia in České Budějovice Branišovská 31 37005České Budějovice Czech Republic

Institute of Parasitology Biology Centre Czech Academy of Sciences 37005České Budějovice Czech Republic

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figshare
10.6084/m9.figshare.17091161.v1