Antibodies against Phlebotomus perniciosus sandfly salivary gland homogenate (SGH) and recombinant protein rSP03B, sandfly-borne Toscana virus (TOSV), Sandfly Fever Sicilian virus (SFSV) and Leishmania, as well as DNA of the latter parasite, were investigated in 670 blood samples from 575 human donors in Murcia Region, southeast Spain, in 2017 and 2018. The estimated SGH and rSP03B seroprevalences were 69% and 88%, respectively, although correlation between test results was relatively low (ρ = 0.39). Similarly, TOSV, SFSV and Leishmania seroprevalences were 26%, 0% and 1%, respectively, and Leishmania PCR prevalence was 2%. Prevalences were significantly greater in 2017, overdispersed and not spatially related to each other although both were positively associated with SGH but not to rSP03B antibody optical densities, questioning the value of the latter as a diagnostic marker for these infections in humans.

Departamento de Sanidad Animal Facultad de Veterinaria Campus de Excelencia Internacional Regional 'Campus Mare Nostrum' Universidad de Murcia Murcia Spain

Department of Parasitology Faculty of Science Charles University Prague Czech Republic

Grupo de Erosión y Conservación de Suelos Centro de Edafología y Biología Aplicada del Segura Consejo Superior de Investigaciones Científicas Murcia Spain

Servicio de Microbiología Hospital Universitario 'Virgen de la Arrixaca' Murcia Spain

Unite des Virus Emergents Aix Marseille Université IRD 190 Inserm 1207 IHU Mediterranée Infection Marseille France

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