Individual variability of salivary gland proteins in three Phlebotomus species
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22197318
DOI
10.1016/j.actatropica.2011.12.004
PII: S0001-706X(11)00361-5
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Phenotype MeSH
- Crosses, Genetic MeSH
- Molecular Weight MeSH
- Phlebotomus chemistry MeSH
- Proteome analysis MeSH
- Cluster Analysis MeSH
- Salivary Proteins and Peptides analysis chemistry MeSH
- Salivary Glands chemistry MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Proteome MeSH
- Salivary Proteins and Peptides MeSH
Pooled salivary gland samples are frequently used to ensure the sufficient amount of material for the experiments; however, this could mask an individual variability. Thus, we compared salivary protein profiles in seven colonies of three Phlebotomus species: Phlebotomus sergenti, Phlebotomus perniciosus, and Phlebotomus papatasi. Surprisingly, the individual profiles differed significantly between the colonies as well as between individuals. The highest variability was observed in proteins with molecular masses of 42-46 kDa corresponding to the yellow-related proteins. The phenogram constructed from salivary gland profiles revealed the existence of two main groups in P. sergenti, corresponding well with the geographical origin. The F1 progeny obtained from cross-mating studies between P. sergenti colonies of different geographical origin formed a distinct subgroup within the parental groups. In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed.
References provided by Crossref.org
The Diversity of Yellow-Related Proteins in Sand Flies (Diptera: Psychodidae)
