Caryophyllideans are intestinal parasites of freshwater fishes, occupying a basal position among the ‘true’ tapeworms. We performed detailed cytogenetic analyses of the well-known caryophyllidean species Caryophyllaeus laticeps. For comparison, we also examined for the first time the chromosomes of Paracaryophyllaeus gotoi, a specific parasite of loaches in China. Both species showed a diploid chromosome number of 2n = 20, n = 10m. Chromomycin A3 (CMA3)/diamidino-2-phenylindole (DAPI) staining performed for the first time in the class Cestoda revealed CMA3+/DAPI− bands in the pericentromeric regions of the short arms of chromosome pair no. 7 in the karyotype of C. laticeps. Fluorescence in situ hybridization with the 18S rDNA probe confirmed the presence of a single cluster of major rDNA near the centromere on a pair of small chromosomes in both species. These findings support the hypothesis that the ancestral state in the family Caryophyllaeidae is a single interstitial cluster of major rDNA genes and thus one nucleolar organizer region per haploid genome. Our results, which we presented together with literature data plotted on a phylogenetic tree, show stability of caryophyllidean karyotypes at the genus level, but showed differences between genera without a clear phylogenetic signal. The data allowed us to at least formulate a hypothesis about the ancestral haploid chromosome number of n = 10 for the family Caryophyllaeidae and possibly for the sister family Capingentidae. In addition, we compared two populations of C. laticeps from water bodies with different levels of polychlorinated biphenyl contamination, showing a slightly increased incidence of chromosomal abnormalities at the contaminated site.

Biology Centre of the Czech Academy of Sciences Institute of Entomology Branišovská 31 370 05 České Budějovice Czech Republic

Institute of Parasitology Slovak Academy of Sciences Hlinkova 3 040 01 Košice Slovakia

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Bajer A (1959) Change of length and volume of mitotic chromosomes in living cells. Hereditas 45, 579–596.

Barčák D, Oros M, Hanzelová V and Scholz T (2017) A synoptic review of PubMed

Bazsalovicsová E, Kráľová-Hromadová I, Brabec J, Hanzelová V, Oros M and Scholz T (2014) Conflict between morphology and molecular data: a case of the genus PubMed

Bombarová M and Špakulová M (2015) New chromosome characteristics of the monozoic tapeworm

Brázová T, Miklisová D, Barčák D, Uhrovič D, Šalamún P, Orosová M and Oros M (2021) Hazardous pollutants in the environment: fish host–parasite interactions and bioaccumulation of polychlorinated biphenyls. Environmental Pollution 291, 118175. PubMed

Chuiko GM, Tillitt DE, Zajicek JL, Flerov BA, Stepanova VM, Zhelnin YY and Podgornaya VA (2007) Chemical contamination of the Rybinsk reservoir, northwest Russia: relationship between live polychlorinated biphenyls (PCB) content and health indicators in bream ( PubMed

Doanh PN, Shinohara A, Horii Y, Yahiro S, Habe S, Vannavong N, Strobel M, Nakamura S and Nawa S (2009) Morphological differences and molecular similarities between PubMed

Dobigny G, Ozouf-Costaz C, Bonillo C and Volobuev V (2002) ‘Ag-NORs’ are not always true NORs: new evidence in mammals. Cytogenetic and Genome Research 98, 75–77. PubMed

Grey AJ (1979) A Comparative Study of the Chromosomes of Twenty Species of Caryophyllidean Tapeworms (Dissertation). College of Arts and Sciences, Department of Biology, State University of New York, Albany, USA, p. 214.

Grey AJ and Mackiewicz JS (1974) Chromosomes of the caryophyllidean tapeworm PubMed

Grey AJ and Mackiewicz JS (1980) Chromosomes of the caryophyllidean cestodes: diploidy, triploidy and parthenogenesis in

Hanzelová V, Oros M, Barčák D, Miklisová D, Kirin D and Scholz T (2015) Morphological polymorphism in tapeworms: redescription of PubMed

Hooper DM and Price PD (2017) Chromosomal inversion differences correlate with range overlap in passerine birds. Nature Ecology & Evolution 1, 1526–1534. PubMed

Kato A, Albert PS, Vega JM and Birchler JA (2006) Sensitive fluorescence PubMed

Kozlovskaya VI and German AV (1997) Polychlorinated biphenyls and polycyclic aromatic hydrocarbons in ecosystem of Rybinsk reservoir. Water Resources 24, 563–569 (in Russian).

Kráľová-Hromadová I, Štefka J, Špakulová M, Orosová M, Bombarová M, Hanzelová V, Bazsalovicsová E and Scholz T (2010) Intraindividual ITS1 and ITS2 ribosomal sequence variation linked with multiple rDNA loci: a case of triploid PubMed

Levan A, Fredga K and Sandberg A (1964) Nomenclature for centromere position on chromosomes. Hereditas 52, 201–220.

Littlewood DTJ and Olson PD (2001) Small subunit rDNA and the Platyhelminthes: signal, noise, conflict and compromise. In Littlewood DTJ and Bray RA (eds), Interrelationships of the Platyhelminthes. London: Taylor and Francis, pp. 262–278.

Motomura I (1929) On the early development of monozoic cestode,

Naseeb S, Carter Z, Minnis D, Donaldson I, Zeef L and Delneri D (2016) Widespread impact of chromosomal inversions on gene expression uncovers robustness via phenotypic buffering. Molecular Biology and Evolution 33, 1679–1696. PubMed PMC

Ogura I (2004) Half-life of each dioxin and PCB congener in the human body. Organohalogen Compounds 66, 3329–3337.

Oros M, Uhrovič D and Scholz T (2018) A new classification of PubMed

Orosová M and Oros M (2012) Classical and molecular cytogenetics of PubMed

Orosová M and Špakulová M (2018) Tapeworm chromosomes: their value in systematics with instructions for cytogenetic study. Folia Parasitologica 65, 001. PubMed

Orosová M, Kráľová-Hromadová I, Bazsalovicsová E and Špakulová M (2010 PubMed

Orosová M, Marec F, Oros M, Xi BW and Scholz T (2010 PubMed

Orosová M, Provazníková I, Xi BW and Oros M (2019) Chromosomal study of PubMed

Orosová M, Marková A, Provazníková I, Oros M, Radačovská A, Čadková Z and Marec F (2021) Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, PubMed PMC

Petkevičiūtė R (1996) A chromosome study of

Petkevičiūtė R (2003) Comparative karyological analysis of three species of PubMed

Petkevičiūtė R and Bondarenko SK (2001) Comparative karyological studies on the species of PubMed

Petkevičiūtė R and Kuperman BI (1992) Karyological investigation of PubMed

Petkevičiūtė R, Stunžėnas V and Stanevičiūtė G (2018) Comments on species divergence in the genus PubMed PMC

Proffitt MR and Jones AW (1969) Chromosome analysis of PubMed

Rábová M, Volker M, Pelikánová Š and Ráb P (2015) Sequential chromosome bandings in fishes. In Ozouf-Costaz C, Pisano E, Foresti F and Toledo LFA (eds), Fish Cytogenetic Techniques, Ray-Fin Fishes and Chondrichthyans. Boca Raton, USA: CRC Press, pp. 66–73.

Sahara K, Marec F and Traut W (1999) TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome Research 76, 449–460. PubMed

Šalgovičová D and Zmetáková Z (2006) Polychlorinated biphenyls in muscle tissue of freshwater fish in East Slovakia. Journal of Food and Nutrition Research 45, 171–178.

Sandal S, Yilmaz B and Carpenter DO (2008) Genotoxic effects of PCB 52 and PCB 77 on cultured human peripheral lymphocytes. Mutation Research 654, 88–92. PubMed

Scholz T, Brabec J, Kráľová-Hromadová I, Oros M, Bazsalovicsová E, Ermolenko A and Hanzelová V (2011) Revision of PubMed

Scholz T, Oros M, Bazsalovicsová E, Brabec J, Waeschenbach A, Xi BW, Aydoğdu A, Besprozvannykh V, Shimazu T, Králová-Hromadová I and Littlewood DTJ (2014) Molecular evidence of cryptic diversity in PubMed

Scholz T, Oros M, Choudhury A, Brabec J and Waeschenbach A (2015) New circumscription of freshwater fish parasites PubMed

Scholz T, Waeschenbach A, Oros M, Brabec J and Littlewood DTJ (2021) Phylogenetic reconstruction of early diverging tapeworms (Cestoda: Caryophyllidea) reveals ancient radiations in vertebrate hosts and biogeographic regions. International Journal for Parasitology 51, 263–277. PubMed

Špakulová M, Orosová M and Mackiewicz JS (2011) Cytogenetics and chromosomes of the tapeworms (Platyhelmithes, Cestoda). Advances in Parasitology 74, 177–230. PubMed

Špakulová M, Bombarová M, Miklisová D, Nechybová S and Langrová I (2019) How to become a successful invasive tapeworm: a case study of abandoned sexuality and exceptional chromosome diversification in the triploid carp parasite PubMed PMC

Sures B, Nachev M, Selbach C and Marcogliese DJ (2017) Parasite responses to pollution: what we know and where we go in ‘Environmental Parasitology’. Parasites & Vectors 10, 65. PubMed PMC

Vijayaraghavan S and Subramanyam S (1977) Chromosome number of the cestode

Wellenreuther M and Bernatchez L (2018) Eco-evolutionary genomics of chromosomal inversions. Trends in Ecology & Evolution 33, 427–440. PubMed

Xi BW, Barčák D, Oros M, Chen K and Xie J (2016) The occurrence of the common European fish cestode PubMed

Zrzavá M, Hladová I, Dalíková M, Šíchová J, Ounap E, Kubíčková S and Marec F (2018) Sex chromosomes of the iconic moth PubMed PMC

Chromosome analysis and the occurrence of B chromosomes in fish parasite Acanthocephalus anguillae (Palaeacanthocephala: Echinorhynchida)

Hooking the scientific community on thorny-headed worms: interesting and exciting facts, knowledge gaps and perspectives for research directions on Acanthocephala