Chromosomal study of Khawia abbottinae (Cestoda: Caryophyllidea): karyotype and localization of telomeric and ribosomal sequences after fluorescence in situ hybridization (FISH)
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31485863
DOI
10.1007/s00436-019-06450-3
PII: 10.1007/s00436-019-06450-3
Knihovny.cz E-zdroje
- Klíčová slova
- 18S rDNA mapping, Cestoda, FISH, Karyotype, Telomeres,
- MeSH
- Cestoda klasifikace genetika izolace a purifikace MeSH
- chromozomy genetika MeSH
- Cyprinidae genetika MeSH
- DNA helmintů genetika MeSH
- heterochromatin genetika MeSH
- hybridizace in situ fluorescenční MeSH
- karyotyp MeSH
- karyotypizace MeSH
- proteiny červů genetika MeSH
- ribozomální DNA genetika MeSH
- ribozomy genetika MeSH
- telomery genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Čína MeSH
- Názvy látek
- DNA helmintů MeSH
- heterochromatin MeSH
- proteiny červů MeSH
- ribozomální DNA MeSH
An original cytogenetic study combining classical karyotype analysis and modern fluorescence in situ hybridization using telomeric (TTAGGG)n and ribosomal sequences (18S rDNA) was performed in Khawia abbottinae (Cestoda, Caryophyllidea), a parasite of Chinese false gudgeon (Abbottina rivularis) from China. Analyses based on conventional Giemsa staining, DAPI, YOYO-1 dye, and silver (Ag) staining were also carried out. The karyotype is composed of eight pairs of metacentric and telocentric chromosomes (2n = 16, n=5m + 3t). Constitutive heterochromatin was mainly positioned at pericentromeric regions, and telomeric sequences (TTAGGG)n were restricted to the end of all chromosomes. In mitotic preparations stained with Giemsa, both homologues of chromosome pair 4 showed a distinct secondary constriction. FISH with rDNA probe confirmed that this secondary constriction contains a nucleolar organizer region (NOR). The process of spermatocyte meiosis and the dynamics of nucleolus degradation in dividing cell were scrutinized. The present study and its results enhance the limited knowledge on basic karyotype characteristics and 18S rDNA clusters location in caryophyllidean tapeworms.
Institute of Entomology Biology Centre CAS Branišovská 31 370 05 České Budějovice Czech Republic
Institute of Parasitology Slovak Academy of Sciences Hlinkova 3 040 01 Košice Slovakia
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