This paper provides a detailed overview of relevant developments in separation and analysis of proteins by capillary electromigration (CE) methods in the period 2017-mid 2021. The presented topics embrace sample preparation, suppression of protein sorption, control of electroosmotic flow, separations of proteins by the particular CE methods and protein identification and quantification by various detection techniques. To illustrate the wide usability of CE methods for protein analysis, the paper shows several novel applications of CE methods, such as quality control and characterization of protein biopharmaceuticals, determination of proteins in complex biomatrices, investigation of posttranslational modification, peptide mapping, interactions of proteins with other (bio)molecules and determination of their physicochemical and biochemical characteristics.

Institute of Organic Chemistry and Biochemistry The Czech Academy of Sciences Flemingovo 542 2 166 10 Prague 6 Czech Republic

References provided by Crossref.org

Does PAD and microcirculation status impact the tissue availability of intravenously administered antibiotics in patients with infected diabetic foot? Results of the DFIATIM substudy

Microdialysis as a tool for antibiotic assessment in patients with diabetic foot: a review

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis