Temporary pools are seasonal wetland habitats with specifically adapted biota, including annual Nothobranchius killifishes that survive habitat desiccation as diapausing eggs encased in dry sediment. To understand the patterns in the structure of Nothobranchius assemblages and their potential in wetland conservation, we compared biodiversity components (alpha, beta, and gamma) between regions and estimated the role and sources of nestedness and turnover on their diversity. We sampled Nothobranchius assemblages from 127 pools across seven local regions in lowland Eastern Tanzania over 2 years, using dip net and seine nets. We estimated species composition and richness for each pool, and beta and gamma diversity for each region. We decomposed beta diversity into nestedness and turnover components. We tested nestedness in three main regions (Ruvu, Rufiji, and Mbezi) using the number of decreasing fills metric and compared the roles of pool area, isolation, and altitude on nestedness. A total of 15 species formed assemblages containing 1-6 species. Most Nothobranchius species were endemic to one or two adjacent regions. Regional diversity was highest in the Ruvu, Rufiji, and Mbezi regions. Nestedness was significant in Ruvu and Rufiji, with shared core (N. melanospilus, N. eggersi, and N. janpapi) and common (N. ocellatus and N. annectens) species, and distinctive rare species. Nestedness apparently resulted from selective colonization rather than selective extinction, and local species richness was negatively associated with altitude. The Nothobranchius assemblages in the Mbezi region were not nested, and had many endemic species and the highest beta diversity driven by species turnover. Overall, we found unexpected local variation in the sources of beta diversity (nestedness and turnover) within the study area. The Mbezi region contained the highest diversity and many endemic species, apparently due to repeated colonizations of the region rather than local diversification. We suggest that annual killifish can serve as a flagship taxon for small wetland conservation.

Department of Botany and Zoology Faculty of Science Masaryk University Brno Czech Republic

Department of Ecology and Vertebrate Zoology University of Łódź Łódź Poland

Department of Fish Ecology and Evolution Centre of Ecology Evolution and Biogeochemistry EAWAG Swiss Federal Institute of Aquatic Science and Technology Kastanienbaum Switzerland

Department of Zoology Faculty of Science Charles University Prague Czech Republic

Institute of Ecology and Evolution University of Bern Bern Switzerland

Institute of Vertebrate Biology Academy of Sciences of the Czech Republic Brno Czech Republic

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Almeida‐Neto, M. , Guimaraes, P. , Guimaraes, P. R. Jr. , Loyola, R. D. , & Ulrich, W. (2008). A consistent metric for nestedness analysis in ecological systems: Reconciling concept and measurement. Oikos, 117, 1227–1239. 10.1111/j.0030-1299.2008.16644.x DOI

Atmar, W. , & Patterson, B. D. (1993). The measure of order and disorder in the distribution of species in fragmented habitat. Oecologia, 96, 373–382. 10.1007/BF00317508 PubMed DOI

Bartáková, V. , Nagy, B. , Polačik, M. , Blažek, R. , Lamtane, H. , & Reichard, M. (2020). Genetic diversity of a widespread annual killifish from coastal Tanzania. BMC Evolutionary Biology, 20(1), 1–13. 10.1186/s12862-019-1549-2 PubMed DOI PMC

Bartáková, V. , Reichard, M. , Blažek, R. , Polačik, M. , & Bryja, J. (2015). Terrestrial fishes: Rivers are barriers to gene flow in annual fishes from the African savanna. Journal of Biogeography, 42, 1832–1844. 10.1111/jbi.12567 DOI

Baselga, A. (2010). Partitioning the turnover and nestedness components of beta diversity. Global Ecology and Biogeography, 19(1), 134–143. 10.1111/j.1466-8238.2009.00490.x DOI

Bevilacqua, S. , & Terlizzi, A. (2020). Nestedness and turnover unveil inverse spatial patterns of compositional and functional β‐diversity at varying depth in marine benthos. Diversity and Distributions, 26, 743–757. 10.1111/ddi.13025 DOI

Brock, M. A. , Nielsen, D. L. , Shiel, R. J. , Green, J. D. , & Langley, J. D. (2003). Drought and aquatic community resilience: the role of eggs and seeds in sediments of temporary wetlands. Freshwater Biology, 48, 1207–1218. 10.1046/j.1365-2427.2003.01083.x DOI

Brualdi, R. A. , & Sanderson, J. G. (1999). Nested species subsets, gaps, and discrepancy. Oecologia, 119, 256–264. 10.1007/s004420050784 PubMed DOI

Cellerino, A. , Valenzano, D. R. , & Reichard, M. (2016). From the bush to the bench: The annual Nothobranchius fishes as a new model system in biology. Biological Reviews, 91, 511–533. PubMed

Costa, W. J. E. M. (2018). Comparative morphology, phylogeny and classification of African seasonal killifishes of the tribe Nothobranchiini (Cyprinodontiformes: Aplocheilidae). Zoological Journal of the Linnean Society, 184(1), 115–135. 10.1093/zoolinnean/zlx102 DOI

Costa, W. J. E. M. (2019). Two new species of seasonal killifishes of the Nothobranchius melanospilus species complex from the East Africa biodiversity hotspot (Cyprinodontiformes: Aplocheilidae). Vertebrate Zoology, 69(1), 73–82.

Dalu, T. , & Wasserman, R. J. (2022). Fundamentals of tropical freshwater wetlands: From ecology to conservation management. Elsevier.

Dalu, T. , Wasserman, R. J. , & Dalu, M. T. (2017). Agricultural intensification and drought frequency increases may have landscape‐level consequences for ephemeral ecosystems. Global Change Biology, 23, 983–985. 10.1111/gcb.13549 PubMed DOI

Dorado, J. , Vázquez, D. P. , Stevani, E. L. , & Chacoff, N. P. (2011). Rareness and specialization in plant‐pollinator networks. Ecology, 92(1), 19–25. 10.1890/10-0794.1 PubMed DOI

Freiry, R. F. , Gouvea, A. , Becker, J. , Lansac‐Tôha, F. A. , Lansac‐Tôha, F. M. , Pires, M. M. , Stenert, C. , & Maltchik, L. (2020). Community structure and concordance patterns among zooplankton life stages in subtropical temporary ponds. Aquatic Ecology, 54(1), 257–270. 10.1007/s10452-019-09740-1 DOI

García, D. , Loureiro, M. , Machín, E. , & Reichard, M. (2019). Species co‐occurrence and population dynamics in annual fish assemblages in the lower Río Uruguay basin. Environmental Biology of Fishes, 102, 569–580. 10.1007/s10641-019-00854-x DOI

García, G. , Loureiro, M. , Berois, N. , Arezo, M. J. , Casanova, G. , Clivio, G. , & Olivera, A. (2009). Pattern of differentiation in the annual killifish genus Austrolebias (Cyprinodontiformes: Rivulidae) from a biosphere reserve site in South America: A multidisciplinary approach. Biological Journal of the Linnean Society, 98, 620–635. 10.1111/j.1095-8312.2009.01303.x DOI

García‐Navas, V. , Martínez‐Núñez, C. , Tarifa, R. , Molina‐Pardo, J. L. , Valera, F. , Salido, T. , Camacho, F. M. , & Rey, P. J. (2022). Partitioning beta diversity to untangle mechanisms underlying the assembly of bird communities in Mediterranean olive groves. Diversity and Distributions, 28(1), 112–127. 10.1111/ddi.13445 DOI

Gardner, R. C. , & Finlayson, M. (2018). Global wetland outlook: State of the world's wetlands and their services to people 2018. Secretariat of the Ramsar Convention.

Gibson, L. A. , Cowan, M. A. , Lyons, M. N. , Palmer, R. , Pearson, D. J. , & Doughty, P. (2017). Island refuges: Conservation significance of the biodiversity patterns resulting from ‘natural’fragmentation. Biological Conservation, 212, 349–356. 10.1016/j.biocon.2017.06.010 DOI

Helmstetter, A. J. , Papadopulos, A. S. , Igea, J. , & Van Dooren, T. J. (2020). Trait evolution and historical biogeography shape assemblages of annual killifish. Journal of Biogeography, 47, 1955–1965. 10.1111/jbi.13912 DOI

Hill, M. J. , Heino, J. , Thornhill, I. , Ryves, D. B. , & Wood, P. J. (2017). Effects of dispersal mode on the environmental and spatial correlates of nestedness and species turnover in pond communities. Oikos, 126, 1575–1585. 10.1111/oik.04266 DOI

Lambert, J. W. , Reichard, M. , & Pincheira‐Donoso, D. (2019). Live fast, diversify non‐adaptively: Evolutionary diversification of exceptionally short‐lived annual killifishes. BMC Evolutionary Biology, 19(1), 10. 10.1186/s12862-019-1344-0 PubMed DOI PMC

Lévêque, C. (1997). Biodiversity dynamics and conservation: The freshwater fish of tropical Africa. Cambridge University Press.

Loiseau, N. , Mouquet, N. , Casajus, N. , Grenié, M. , Guéguen, M. , Maitner, B. , Mouillot, D. , Ostling, A. , Renaud, J. , Tucker, C. , Velez, L. , Thuiller, W. , & Violle, C. (2020). Global distribution and conservation status of ecologically rare mammal and bird species. Nature Communications, 11(1), 1–11. 10.1038/s41467-020-18779-w PubMed DOI PMC

Loureiro, M. , Borthagaray, A. , Hernández, D. , Duarte, A. , Pinelli, V. , & Arim, M. (2015). Austrolebias in space: Scaling from ponds to biogeographical regions. In Berois N., García G., & de Sá R. (Eds.), Annual fishes: Life history strategy, diversity, and evolution (pp. 111–132). CRC Press.

Magurran, A. E. (2003). Measuring biological diversity. John Wiley & Sons.

Markham, J. (2014). Rare species occupy uncommon niches. Scientific Reports, 4(1), 1–3. PubMed PMC

Nagy, B. , & Kis, A. (2010). Variation in habitat characteristics and the occurrence of Nothobranchius species in seasonal biotopes of Tanzania. JAKA, 43, 130–144.

Nagy, B. , & Watters, B. (2021). A review of the conservation status of seasonal Nothobranchius fishes (Teleostei: Cyprinodontiformes), a genus with a high level of threat, inhabiting ephemeral wetland habitats in Africa. Aquatic Conservation: Marine and Freshwater Ecosystems, 32(1), 199–216.

Oksanen, J. , Blanchet, F. G. , Kindt, R. , Legendre, P. , Minchin, P. R. , O’Hara, R. B. , Simpson, G. L. , Stevenes, M. H. H. , & Wagner, H. H. (2013). Package ‘vegan’. Community ecology package, 2, 1‐295.

Patterson, B. D. , & Atmar, W. (1986). Nested subsets and the structure of insular mammalian faunas and archipelagos. Biological Journal of the Linnean Society, 28(1–2), 65–82. 10.1111/j.1095-8312.1986.tb01749.x DOI

Pepke, M. L. , Irestedt, M. , Fjeldså, J. , Rahbek, C. , & Jønsson, K. A. (2019). Reconciling supertramps, great speciators and relict species with the taxon cycle stages of a large island radiation (Aves: Campephagidae). Journal of Biogeography, 46, 1214–1225. 10.1111/jbi.13577 DOI

Polačik, M. , Harrod, C. , Blažek, R. , & Reichard, M. (2014). Trophic niche partitioning in communities of African annual fish: Evidence from stable isotopes. Hydrobiologia, 721, 99–106. 10.1007/s10750-013-1652-0 DOI

Polačik, M. , & Reichard, M. (2010). Diet overlap among three sympatric African annual killifish species Nothobranchius spp. from Mozambique. Journal of Fish Biology, 77, 754–768. 10.1111/j.1095-8649.2010.02717.x PubMed DOI

Polačik, M. , Vrtílek, M. , Reichard, M. , Žák, J. , Blažek, R. , & Podrabsky, J. (2021). Embryo ecology: Developmental synchrony and asynchrony in the embryonic development of wild annual fish populations. Ecology and Evolution, 11, 4945–4956. 10.1002/ece3.7402 PubMed DOI PMC

R Core Team . (2018). R: A language and environment for statistical computing. R Foundation for Statistical Computing.

Reichard, M. (2015). Annual fishes: Life history strategy, diversity, and evolution. In Berois N., Garcia G., & De Sa R. O. (Eds.), The evolutionary ecology of African annual fishes (pp. 133–158). CRC Press, Taylor and Francis group.

Reichard, M. , Janáč, M. , Polačik, M. , Blažek, R. , & Vrtílek, M. (2017). Community assembly in Nothobranchius annual fishes: Nested patterns, environmental niche and biogeographic history. Ecology and Evolution, 7, 2294–2306. PubMed PMC

Reichard, M. , & Polačik, M. (2019). The Natural History of Model Organisms: Nothobranchius Furzeri, an ‘instant’ Fish from an Ephemeral Habitat. eLife, 8, e41548. 10.7554/eLife.41548 PubMed DOI PMC

Reichard, M. , Polačik, M. , Blažek, R. , & Vrtílek, M. (2014). Female bias in the adult sex ratio of African annual fishes: Interspecific differences, seasonal trends and environmental predictors. Evolutionary Ecology, 28, 1105–1120. 10.1007/s10682-014-9732-9 DOI

Roberts, T. R. (1975). Geographical distribution of African freshwater fishes. Zoological Journal of the Linnean Society, 57, 249–319. 10.1111/j.1096-3642.1975.tb01893.x DOI

Shidlovsky, K. (2010). Collecting Nothobranchius in south‐western Tanzania. Nothobranchius Archives, 1, 18–27.

Socolar, J. B. , Gilroy, J. J. , Kunin, W. E. , & Edwards, D. P. (2016). How should beta‐diversity inform biodiversity conservation? Trends in Ecology & Evolution, 31(1), 67–80. 10.1016/j.tree.2015.11.005 PubMed DOI

Soininen, J. , Heino, J. , & Wang, J. (2018). A meta‐analysis of nestedness and turnover components of beta diversity across organisms and ecosystems. Global Ecology and Biogeography, 27(1), 96–109. 10.1111/geb.12660 DOI

Ulrich, W. (2009). Nestedness analysis as a tool to identify ecological gradients. Ecological Questions, 11, 27–34. 10.12775/v10090-009-0015-y DOI

van der Merwe, P. D. W. , Cotterill, F. P. D. , Kandziora, M. , Watters, B. R. , Nagy, B. , Genade, T. , Flügel, T. J. , Svendsen, D. S. , & Bellstedt, D. U. (2021). Genomic fingerprints of Palaeogeographic history: The tempo and mode of Rift tectonics across tropical Africa has shaped the diversification of the killifish genus Nothobranchius (Teleostei: Cyprinodontiformes). Molecular Phylogenetics and Evolution, 158, 106988. 10.1016/j.ympev.2020.106988 PubMed DOI

van der Valk, A. (2012). The biology of freshwater wetlands. Oxford University Press.

Vanschoenwinkel, B. , Waterkeyn, A. , Nhiwatiwa, T. , Pinceel, T. , Spooren, E. , Geerts, A. , Clegg, B. , & Brendonck, L. (2011). Passive external transport of freshwater invertebrates by elephant and other mud‐wallowing mammals in an African savannah habitat. Freshwater Biology, 56, 1606–1619. 10.1111/j.1365-2427.2011.02600.x DOI

Vrtílek, M. , Žák, J. , Pšenička, M. , & Reichard, M. (2018). Extremely rapid maturation of a wild African annual fish. Current Biology, 28, R822–R824. 10.1016/j.cub.2018.06.031 PubMed DOI

Watters, B. R. , Nagy, B. , van der Merwe, P. D. , Cotterill, F. P. , & Bellstedt, D. U. (2020). Redescription of the seasonal killifish species Nothobranchius ocellatus and description of a related new species Nothobranchius matanduensis, from eastern Tanzania. Ichthyological Exploration of Freshwaters, 30, 151–178.

Wildekamp, R. (2004). A world of killies (vol. 4). Atlas of the Oviparous Cyprinodontiform fishes of the world. NHBS Academic & Professional Books.

Wildekamp, R. W. (2019). Back to the theme Nothobranchius guentheri and Nothobranchius melanospilus, with additional notes to the N. melanospilus complex sensu Costa 2019. JAKA, 52, 43–52.

Williams, D. D. (2006). The biology of temporary waters. Oxford University Press.

Wright, D. H. , Patterson, B. D. , Mikkelson, G. M. , Cutler, A. , & Atmar, W. (1997). A comparative analysis of nested subset patterns of species composition. Oecologia, 113(1), 1–20. 10.1007/s004420050348 PubMed DOI

Xu, A. , Han, X. , Zhang, X. , Millien, V. , & Wang, Y. (2017). Nestedness of butterfly assemblages in the Zhoushan Archipelago, China: Area effects, life‐history traits and conservation implications. Biodiversity and Conservation, 26, 1375–1392. 10.1007/s10531-017-1305-0 DOI

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