Green methods have become vital for sustainable development of the scientific and commercial sphere; however, they can bring new challenges, including the need for detailed characterization and elucidation of efficacy of their products. In this study, green method of silver nanoparticles (AgNPs) production was employed using an extract from grapevine canes. The aim of the study was to contribute to the knowledge about biosynthesized AgNPs by focusing on elucidation of their antifungal efficiency based on their size and/or hypothesized synergy with bioactive substances from Vitis vinifera cane extract. The antifungal activity of AgNPs capped and stabilized with bioactive compounds was tested against the opportunistic pathogenic yeast Candida albicans. Two dispersions of nanoparticles with different morphology (characterized by SEM-in-STEM, DLS, UV-Vis, XRD, and AAS) were prepared by modification of reaction conditions suitable for economical production and their long-term stability monitored for six months was confirmed. The aims of the study included the comparison of the antifungal effect against suspension cells and biofilm of small monodisperse AgNPs with narrow size distribution and large polydisperse AgNPs. The hypothesis of synergistic interaction of biologically active molecules from V. vinifera extracts and AgNPs against both cell forms were tested. The interactions of all AgNPs dispersions with the cell surface and changes in cell morphology were imaged using SEM. All variants of AgNPs dispersions were found to be active against suspension and biofilm cells of C. albicans; nevertheless, surprisingly, larger polydisperse AgNPs were found to be more effective. Synergistic action of nanoparticles with biologically active extract compounds was proven for biofilm cells (MBIC80 20 mg/L of polydisperse AgNPs in extract), while isolated nanoparticles suspended in water were more active against suspension cells (MIC 20 mg/L of polydisperse AgNPs dispersed in water). Our results bring new insight into the economical production of AgNPs with defined characteristics, which were proven to target a specific mode of growth of significant pathogen C. albicans.

Department of Biotechnology University of Chemistry and Technology Prague Czech Republic

Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic

Research Centre Řež Husinec Czech Republic

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Dikshit PK, Kumar J, Das AK, Sadhu S, Sharma S, Singh S, et al.. Green synthesis of metallic nanoparticles: Applications and limitations. Catalysts. 2021;11(8):902.

Gour A, Jain NK. Advances in green synthesis of nanoparticles. Artificial cells, nanomedicine, and biotechnology. 2019;47(1):844–51. doi: 10.1080/21691401.2019.1577878 PubMed DOI

Kolouchova I, Melzoch K, Šmidrkal J, Filip V. The content of resveratrol in vegetables and fruit. Chemické listy. 2005;99(7).

Dauthal P, Mukhopadhyay M. Noble metal nanoparticles: plant-mediated synthesis, mechanistic aspects of synthesis, and applications. Industrial & Engineering Chemistry Research. 2016;55(36):9557–77.

Rajput H, Kedia A, Shah D, Gamit HA, Amaresan N. Sustainable synthesis of silver nanoparticles using fruit waste and its antibacterial activity. Materials Today: Proceedings. 2022, in press.

Rani P, Ahmed B, Singh J, Kaur J, Rawat M, Kaur N, et al.. Silver nanostructures prepared via novel green approach as an effective platform for biological and environmental applications. Saudi journal of biological sciences. 2022;29(6):103296. doi: 10.1016/j.sjbs.2022.103296 PubMed DOI PMC

Ali K, Ahmed B, Dwivedi S, Saquib Q, Al-Khedhairy AA, Musarrat J. Microwave accelerated green synthesis of stable silver nanoparticles with Eucalyptus globulus leaf extract and their antibacterial and antibiofilm activity on clinical isolates. PloS one. 2015;10(7):e0131178. doi: 10.1371/journal.pone.0131178 PubMed DOI PMC

Makarov V, Love A, Sinitsyna O, Makarova S, Yaminsky I, Taliansky M, et al.. “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae. 2014;6(1 (20)):35–44. PubMed PMC

Al-Otibi F, Alfuzan SA, Alharbi RI, Al-Askar AA, Al-Otaibi RM, Al Subaie HF, et al.. Comparative study of antifungal activity of two preparations of green silver nanoparticles from Portulaca oleracea extract. Saudi Journal of Biological Sciences. 2022;29(4):2772–81. doi: 10.1016/j.sjbs.2021.12.056 PubMed DOI PMC

Borase HP, Salunke BK, Salunkhe RB, Patil CD, Hallsworth JE, Kim BS, et al.. Plant extract: a promising biomatrix for ecofriendly, controlled synthesis of silver nanoparticles. Applied biochemistry and biotechnology. 2014;173(1):1–29. doi: 10.1007/s12010-014-0831-4 PubMed DOI

Vijayaraghavan K, Ashokkumar T. Plant-mediated biosynthesis of metallic nanoparticles: a review of literature, factors affecting synthesis, characterization techniques and applications. Journal of environmental chemical engineering. 2017;5(5):4866–83.

Maťátková O, Michailidu J, Miškovská A, Kolouchová I, Masák J, Čejková A. Antimicrobial properties and applications of metal nanoparticles biosynthesized by green methods. Biotechnology advances. 2022:107905. doi: 10.1016/j.biotechadv.2022.107905 PubMed DOI

Khan A, Ameen F, Khan F, Al-Arfaj A, Ahmed B. Fabrication and antibacterial activity of nanoenhanced conjugate of silver (I) oxide with graphene oxide. Materials Today Communications. 2020;25:101667.

Deshmukh SP, Patil S, Mullani S, Delekar S. Silver nanoparticles as an effective disinfectant: A review. Materials Science and Engineering: C. 2019;97:954–65. doi: 10.1016/j.msec.2018.12.102 PubMed DOI PMC

Haroon M, Zaidi A, Ahmed B, Rizvi A, Khan MS, Musarrat J. Effective inhibition of phytopathogenic microbes by eco-friendly leaf extract mediated silver nanoparticles (AgNPs). Indian journal of microbiology. 2019;59(3):273–87. doi: 10.1007/s12088-019-00801-5 PubMed DOI PMC

Ahmed B, Hashmi A, Khan MS, Musarrat J. ROS mediated destruction of cell membrane, growth and biofilms of human bacterial pathogens by stable metallic AgNPs functionalized from bell pepper extract and quercetin. Advanced Powder Technology. 2018;29(7):1601–16.

Owaid MN, Rabeea MA, Aziz AA, Jameel MS, Dheyab MA. Mycogenic fabrication of silver nanoparticles using Picoa, Pezizales, characterization and their antifungal activity. Environmental Nanotechnology, Monitoring & Management. 2022;17:100612.

Begum T, Follett PA, Mahmud J, Moskovchenko L, Salmieri S, Allahdad Z, et al.. Silver nanoparticles-essential oils combined treatments to enhance the antibacterial and antifungal properties against foodborne pathogens and spoilage microorganisms. Microbial Pathogenesis. 2022;164:105411. doi: 10.1016/j.micpath.2022.105411 PubMed DOI

Varadharajan V, Ramaswamy A, Shanmugham S. Silver nanoparticle synthesis using grape (Vitis vinifera L.) skin extract and its application as anticancerous agent. Advanced Science, Engineering and Medicine. 2016;8(8):626–34.

Hashim N, Paramasivam M, Tan JS, Kernain D, Hussin MH, Brosse N, et al.. Green mode synthesis of silver nanoparticles using Vitis vinifera’s tannin and screening its antimicrobial activity/apoptotic potential versus cancer cells. Materials Today Communications. 2020;25:101511.

Gnanajobitha G, Paulkumar K, Vanaja M, Rajeshkumar S, Malarkodi C, Annadurai G, et al.. Fruit-mediated synthesis of silver nanoparticles using Vitis vinifera and evaluation of their antimicrobial efficacy. Journal of Nanostructure in Chemistry. 2013;3(1):1–6.

Rollová M, Gharwalova L, Krmela A, Schulzová V, Hajšlová J, Jaroš P, et al.. Grapevine extracts and their effect on selected gut-associated microbiota: In vitro study. Czech Journal of Food Sciences. 2020;38(3):137–43.

ImageJ. 1.53g - FIJI, Java 1.8.0_66 (64-bit). Property rights: Wayne Rasband and contributors, National Institutes of Health, USA, http://imagej.nih.gov/ij.

Serra E, Hidalgo-Bastida LA, Verran J, Williams D, Malic S. Antifungal activity of commercial essential oils and biocides against Candida albicans. Pathogens. 2018;7(1):15. doi: 10.3390/pathogens7010015 PubMed DOI PMC

Vaňková E, Kašparová P, Dulíčková N, Čeřovský V. Combined effect of lasioglossin LL-III derivative with azoles against Candida albicans virulence factors: biofilm formation, phospholipases, proteases and hemolytic activity. FEMS yeast research. 2020;20(3):foaa020. doi: 10.1093/femsyr/foaa020 PubMed DOI

Khan I, Saeed K, Khan I. Nanoparticles: Properties, applications and toxicities. Arabian journal of chemistry. 2019;12(7):908–31.

www.nanocomposix.com. Silver Nanoparticles: Optical Properties 2014 [cited 2021. https://nanocomposix.com/pages/silver-nanoparticles-optical-properties#toc. (accessed Nov 01, 2021).

Alsammarraie FK, Wang W, Zhou P, Mustapha A, Lin M. Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids and Surfaces B: Biointerfaces. 2018;171:398–405. doi: 10.1016/j.colsurfb.2018.07.059 PubMed DOI

Mukherji S, Bharti S, Shukla G, Mukherji S. 1. Synthesis and characterization of size-and shape-controlled silver nanoparticles. Metallic Nanomaterials (Part B). 2018:1–116.

Shnoudeh AJ, Hamad I, Abdo RW, Qadumii L, Jaber AY, Surchi HS, et al. Synthesis, characterization, and applications of metal nanoparticles. Biomaterials and bionanotechnology: Elsevier; 2019. p. 527–612.

Forbes NA, Yuan TT, DeSilva MN. Silver Nanoparticle Storage Stability in Aqueous and Biological Media. Naval Medical Research Unit San Antonio, Joint Base San Antonio, Fort Sam, Houston United States 2015.

Abdelwahed W, Degobert G, Stainmesse S, Fessi H. Freeze-drying of nanoparticles: formulation, process and storage considerations. Advanced drug delivery reviews. 2006;58(15):1688–713. doi: 10.1016/j.addr.2006.09.017 PubMed DOI

Mukherji S, Bharti S, Shukla G, Mukherji S. Synthesis and characterization of size-and shape-controlled silver nanoparticles. Physical Sciences Reviews. 2019;4(1).

Gherasim O, Puiu RA, Bîrcă AC, Burdușel A-C, Grumezescu AM. An updated review on silver nanoparticles in biomedicine. Nanomaterials. 2020;10(11):2318. doi: 10.3390/nano10112318 PubMed DOI PMC

Choi JS, Lee JW, Shin UC, Lee MW, Kim DJ, Kim SW. Inhibitory activity of silver nanoparticles synthesized using Lycopersicon esculentum against biofilm formation in Candida species. Nanomaterials. 2019;9(11):1512. doi: 10.3390/nano9111512 PubMed DOI PMC

Wypij M, Czarnecka J, Świecimska M, Dahm H, Rai M, Golinska P. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World Journal of Microbiology and Biotechnology. 2018;34(2):1–13. doi: 10.1007/s11274-017-2406-3 PubMed DOI PMC

Jain J, Arora S, Rajwade JM, Omray P, Khandelwal S, Paknikar KM. Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. Molecular pharmaceutics. 2009;6(5):1388–401. doi: 10.1021/mp900056g PubMed DOI

Zhang L, Wu L, Si Y, Shu K. Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization. PLoS One. 2018;13(12):e0209020. doi: 10.1371/journal.pone.0209020 PubMed DOI PMC

Nisar P, Ali N, Rahman L, Ali M, Shinwari ZK. Antimicrobial activities of biologically synthesized metal nanoparticles: an insight into the mechanism of action. JBIC Journal of Biological Inorganic Chemistry. 2019;24(7):929–41. doi: 10.1007/s00775-019-01717-7 PubMed DOI

Wang X, Wu H-F, Kuang Q, Huang R-B, Xie Z-X, Zheng L-S. Shape-dependent antibacterial activities of Ag2O polyhedral particles. Langmuir. 2010;26(4):2774–8. doi: 10.1021/la9028172 PubMed DOI

Cheon JY, Kim SJ, Rhee YH, Kwon OH, Park WH. Shape-dependent antimicrobial activities of silver nanoparticles. International journal of nanomedicine. 2019;14:2773. doi: 10.2147/IJN.S196472 PubMed DOI PMC

Chaffin WL, López-Ribot JL, Casanova M, Gozalbo D, Martínez JP. Cell wall and secreted proteins of Candida albicans: identification, function, and expression. Microbiology and Molecular Biology Reviews. 1998;62(1):130–80. doi: 10.1128/MMBR.62.1.130-180.1998 PubMed DOI PMC

Slavin YN, Asnis J, Häfeli UO, Bach H. Metal nanoparticles: understanding the mechanisms behind antibacterial activity. Journal of nanobiotechnology. 2017;15(1):1–20. PubMed PMC

Radhakrishnan VS, Mudiam MKR, Kumar M, Dwivedi SP, Singh SP, Prasad T. Silver nanoparticles induced alterations in multiple cellular targets, which are critical for drug susceptibilities and pathogenicity in fungal pathogen (Candida albicans). International journal of nanomedicine. 2018;13:2647. doi: 10.2147/IJN.S150648 PubMed DOI PMC

Santos ALSd, Galdino ACM, Mello TPd, Ramos LdS, Branquinha MH, Bolognese AM, et al.. What are the advantages of living in a community? A microbial biofilm perspective! Memórias do Instituto Oswaldo Cruz. 2018;113(9). PubMed PMC

Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. The lancet. 2001;358(9276):135–8. doi: 10.1016/s0140-6736(01)05321-1 PubMed DOI

Van Hengel I, Tierolf M, Valerio V, Minneboo M, Fluit A, Fratila-Apachitei L, et al.. Self-defending additively manufactured bone implants bearing silver and copper nanoparticles. Journal of Materials Chemistry B. 2020;8(8):1589–602. doi: 10.1039/c9tb02434d PubMed DOI

Ansari MA, Kalam A, Al-Sehemi AG, Alomary MN, AlYahya S, Aziz MK, et al.. Counteraction of Biofilm Formation and Antimicrobial Potential of Terminalia catappa Functionalized Silver Nanoparticles against Candida albicans and Multidrug-Resistant Gram-Negative and Gram-Positive Bacteria. Antibiotics. 2021;10(6):725. doi: 10.3390/antibiotics10060725 PubMed DOI PMC

Lara HH, Romero-Urbina DG, Pierce C, Lopez-Ribot JL, Arellano-Jiménez MJ, Jose-Yacaman M. Effect of silver nanoparticles on Candida albicans biofilms: an ultrastructural study. Journal of nanobiotechnology. 2015;13(1):1–12. PubMed PMC

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