High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Cell surface phenotyping, Fluorescent cell barcoding, High-throughput screening, Multicolor flow cytometry,
- MeSH
- Antigens, Surface * MeSH
- Biomarkers analysis MeSH
- Fluorescent Dyes MeSH
- Flow Cytometry MeSH
- Research * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Surface * MeSH
- Biomarkers MeSH
- Fluorescent Dyes MeSH
Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.
Department of Experimental Biology Faculty of Science Masaryk University Brno Czech Republic
Human Oncology and Pathogenesis Program Memorial Sloan Kettering Cancer Center New York NY USA
International Clinical Research Center St Anne's University Hospital in Brno Brno Czech Republic
See more in PubMed
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